| Project/Area Number |
08672013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Fukui Medical University |
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Principal Investigator |
AKAGI Yoshio Fukui Medical University, Ophthalmology, Professor, 医学部, 教授 (70133189)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Yukio Fukui Medical University, Ophthalmology, Assistant Professor, 医学部, 助教授 (80163294)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Rat galactosemic cataract / Lens epithelium / Histology of lens / PCNA / DAPI (Fluorescence Cytophotometry) / ラットガラクトース白内障 / 抗PCNA抗体酵素抗体法 / DNA染色螢光顕微測光法 / 水晶体線維膨化 / 2倍体細胞 |
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| Research Abstract |
Cell growth of rat lens epithelium during galactose-induced cataract formation was examined in the present study using fluorescence cytophotometry or proliferating cell nuclear antigen (PCNA) immunohistochemistry. Six-week old male Sprague-Dawley rats were divided into 4 dietary groups according to galactose supplementation of the diet : 5%, 10%, 15% and 25% galactose diet groups. Whole-mount preparations of rat lens epithelium were prepared. The nuclear content of epithelial cells was measured using fluorescence cytophotometry, and cells undergoing DNA synthesis (PCNA labeled cells) were detected using the PCNA immunohistochemical staining method. The results of fluorescence cytophotometry showed that cells containing tetraploid nuclei (4C cells) gradually increased in number. In the 5% galactose diet fed rats, the number of 4C cells peaked on the 9th day after the start of dietary galactose supplementation, the 7th day in the 10% galactose diet fed rats and on the 5th day in the 15% and 25% galactose diet fed rats. On each day that 4C cells peaked, a large number of PCNA labeled cells were observed in the central zone of the epithelial cell layr. These results suggest that growth stimulation of lens epithelial cells depends upon the concentration of galactose in the diet and precedes changes in lens fiber.
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