Development of drug therapy on choroidal neovascularization
| Project/Area Number |
08672016
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
KITA Mihori Kyoto University, Faculty of Medicine, Assistant Professor, 医学研究科, 助手 (00252453)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OGURA Yuichiro Nagoya City University, Medical School, Professor, 医学部, 教授 (70191963)
HONDA Yoshihito Kyoto University, Faculty of Medicine, Professor, 医学研究科, 教授 (90026930)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | Pigmented rat / Choroidal neovascularization / Thalidomide / Diode laser / Retinal pigment epithelium / fluorescein fundus angiography / Electroretinogram |
|---|
| Research Abstract |
Thalidomide has been shown to suppress angiogenesis in a rabbit cornea. Our study was undertaken to evaluate the effect of thalidomide on angiogenesis in a rat choroidal neovascularization model. Choroidal neovascularization in pigmented rat eyes was induced by diode laser photocoagulation. Thalidomide (200mg/kg/day) dissolved in dimetyl sulphoxide (DMSO) was given intraperitoneally for 5 days, since 2 days after photocoagulation. Lesions were studied by ophthalmoscopy, fundus photography, and fluorescein fundus angiography at 1 and 2 weeks after photocoagulation. Morphologic correlation was provided by sectioning of lesions for light and transmission electron microscopy. At 2 weeks after photocoagulation, choroidal neovascularization indicated by fluorescein leakage occurred in 37.3% of DMSO treated group, 41.8% of saline treated group, and 51.2% of thalidomide treated group. Histologic examination revealed that choroidal neovascularization was covered with various cells including of retinal pigment epithelial cells, glial cells, fibroblast, and macrophages. Histologic examination and electroretinogram showed no toxicity of thalidomide in this dose. We could find no statistic difference in the incidence of choroidal neovascularization in rat model between treated group and control groups. Further investigation including as to the suppression effect on the extension of the lesion is necessary to estimate the efficacy of thalidomide.
|
Report
(3 results)
Research Products
(4 results)
