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Experimental Study on Aqueous Outflow of the Animal Eyes relating Insults and Intraocular Surgery

Research Project

Project/Area Number 08672018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionYamaguchi university

Principal Investigator

SUZUKI Ryo  Yamaguchi University School of Medicine, Associate Professor, 医学部, 助教授 (50163027)

Co-Investigator(Kenkyū-buntansha) FUJIKURA Yoshihisa  Ohita Medical School, Professor, 医学部, 教授 (10165368)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsglaucoma / trabecular / outflow facility / ciliary muscle / intraocular pressure / adrenergic / intraocular muscle / 隅角線維柱帯 / フォスフォディエステラーゼ阻害薬 / 房水流出抵抗 / 隅角 / 繊維柱帯
Research Abstract

Changes in the outflow facility of perfused bovine eyes, in the shape of cultured trabecular meshwork (TM) and in the responses of the bovine ciliary muscles have been studied after exposure to prostaglandins (PGs), adrenergic drugs, hydrogen peroxides and other substances relating insults and intraocular surgery. Bovine TM tissues were isolated and cultured by the method of Crean et al. Cells were monitored by phase-contrast microscopy.
Dobutamine caused confluent TM cells to change from their usual polygonal shape to a characteristic stellate shape. Salbutamol was without effect. The phosphodiesterase (PDE) in hibitors were very effective in producing this shape change. Epinephrine, isoproterenol or salbutamol did not increase the outflow facility. The facility was 97.3(]SY.+-。[)6.8 (1 muM), 80,8(]SY.+-。[)5.9 (10 muM), 80,8(]SY.+-。[)5.9 (10 muM), 83.5(]SY.+-。[)4.7% (100 muM), respectively, in the case of dobutamine, a beta1 agonist. By contrast, H202 increased facility by 46.5(]SY.+-。[)6.5% (with H202) and 22.1 (]SY.+-。[)7.2% (without H202), especially in the presence of 3 amnotriazole (3AT) and BCNU.PGs also increased the facility, especially in the presence of indomethacin.
The high concentrations of beta-adrenergic agents required to produce even a small change in outflow facility, the cell shape and the accompanying response of ciliary muscles argue against the involvement of adrenergic receptor mediation. The mechanism may be alternatively explained different from beta action. PDE inhibition, PGs and/or oxidative stress playd great influence on and around the outflow channels.
So far, there has been no success in demonstrating such an effect on facility of animal eyes in vitro. However, this study suggests a possibility that such biological substances maay reduce outflow facility of the eye in relation to insults or intraocular surgery, possibly causing untreatable glaucoma.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 鈴木 亮: "Prosteglandinsはin vitroで内眼筋の反応と房水流出率を促進する。" 日本平滑筋学会雑誌. 32. 213-218 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R, Cream E, Anderson P: "Effects of adrenergic agents and phosphodiesterase inhibitors on outflow facility and cell shape of bovine trabecular mesh work." Jap J Oph thalmol. 41. 31-37 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R: "Modified trabeculectomy with a fornix-based flow" Annals of Ophthalmol. 29. 303-306 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R: "Subconjuctival and intrachamber injection of viacoelastic substance to prevent maeked postfiltration hypotony and shallow anterior chamber." Annals of Ophthalmol. 29. 240-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R: "Ocular outflow facility with emphasis on neuronal regulation of intraocular smooth muscles." J Smooth Muscle Res. 32. 229-247 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 鈴木 亮, 藤倉 義久, Anderson PJ: "房水流出率と隅角組織における交感神経作動薬とフォフォディエステラーゼ阻害薬の効果" 日本眼科学会雑誌. 101. 551-557 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R: "Prostaglandins increase the contractile responses of the ciliary muscle and the outflow facility of the bovine eyes." J Smooth Muscle Res. 32. 213-218 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R: "Ocular outflow facility with emphasis on neuronal regulation of intraocular smooth muscles." J Smooth Muscle Res. 32 (3). 229-247 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R,Crea EV,Anderson PJ: "Effects of adrenergic agents and phosphodiesterase inhibitors on outflow facility and cell shape of bovine trabecular meshwork." Jap J Ophthalmol. 41. 31-37 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R,Trabeculectomy: "its care and modification." Ophthalmology. 38. 865-872 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R,Fujikura Y,Anderson PJ: "Different effects of adrenergic agents and phosphodiesterase inhibitors on outflow facility and on bovine trabecular meshwork." Acta Ophthalmol Jap. 101. 551-557 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R: "Trabeculectomy with a Kelly Descemet membrane punch." Ophthalmologica. 211. 93-95 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuroki S,Suzuki R,Fujiwara N: "A comparison of blood pressure changes in phacoemulsification and aspiration between topical and retobulbar block local anesthesia." Graefe Arch Clin Exp Ophthalmol. 236. 277-282 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R: "Subconjunctival and intrachamber injection of viacoelastic substance to prevent marked posifiltration hypotony and shallow anterior chamber." Ann Ophthalmol (Glauc). 29. 240-242 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R: "Phosphodiesterase inhibitors increases outflow facility of the bovine eyes." Atarashii Gannka. 14. 595-599 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki R,Nakayama M and Fravio T: "Two types of massive and prolonged retinal bleeding s a complication of hypotony after trabeculectomy." Ophthalmologica. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 鈴木 亮: "Prostaglandinsはin vitroで内眼筋の反応と房水流出率を促進する。" 日本平滑筋学会雑誌. 32. 213-218 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suzuki R, Cream E, Anderson P: "Effects of adrenergic agents and phosphodiesterase inhibitors on outflow facility and cell shape of bovine trabecular meshwork." Jap J Ophthalmol. 41. 31-37 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suzuki R: "Modified trabeculectomy with a formix-based flap." Annals of Ophthalmol. 29. 303-306 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suzuki R,: "Subconjunctival and intrachamber injection of viacoelastic substance to prevent marked postfiltration hypotony and shallow anterior chamber." Annals of Ophthalmol. 29. 240-242 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suzuki R,: "Ocular outflow facility with emphasis on neuronal regulation of intraocular smooth muscles." J Smooth Muscle Res. 32. 229-247 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鈴木亮, 藤倉義久, Amderson PJ: "房水流出率と隅角組織における交感神経作動薬とフォスフォディ エステラーゼ阻害薬の効果" 日本眼科学会雑誌. 101. 551-557 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鈴木亮: "Prostaglandinsはin vitroで内眼筋の反応と房水流出率を促進する。" 日本平滑筋学会雑誌. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木亮: "房水流出率と毛様体筋に及ぼす交感神経作動薬の効果" あたらしい眼科. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木亮: "房水流出率と隅角組織におけるphoshodiesterase阻害薬の効果(その1)" 日本眼科学会雑誌. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki R: "Adrenergic influence on outflow facilify and shape of cells of bovine trabecular meshwork tissue." Jap J Ophibalmol. (in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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