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Neurobiological Study of Taste Buds

Research Project

Project/Area Number 08672079
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

TOYOSHIMA Kuniaki  Department of Oral Anatomoy II,Kyushu Dental College, Professor, 歯学部, 教授 (10112559)

Co-Investigator(Kenkyū-buntansha) TANDLER Bernard  Department of Oral Anatomoy II,Kyushu Dental College, Assistant Professor, 歯学部, 助手 (90271212)
HARADA Hidemitsu  Department of Oral Anatomoy II,Kyushu Dental College, Assistant Professor, 歯学部, 講師 (70271210)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsTaste bud / Morphogenesis / Serotonin / Ultrastructure / Immunohistochemistry / Merkel cell / 葉状乳頭 / メルケル細胞 / 神経細胞 / 舌 / 有郭乳頭
Research Abstract

Taste organs in the frog have a distinctive cell type located exclusively in the basal portion. In the same fashion as type III cells in mammalian taste buds, these basal cells also show immunoreactivity for serotonin antibody. Further, these cells are morphologically similar to epidermal Merkel cells. To determine the significance of these serotonergic basal cells, we examined the early development of taste organs during metamorphosis of the frog, focusing on the origin and possible roles of serotonergic basal cells. For convenience of description, five stages of development (metamorphic stage to climax stage A to D) are defined. In the metamorphic stage, a few, non-innervated Merkel cells appear at the upper layer of the lingual epithelium. No neuronal elements are seen in the epithelium at this stage. From climax stage A to B, immature fungiform papillae become discernible in the dorsal surface of the tongue where the Merkel cells are located. Merkel cells then move downward and extend their cytoplasmic processes toward the basal lamina. These cells are identified by their intense immunoreactivity for serotonin, During the latter stages, many nerve fibers in the subepithelial connective tissue approach the epithelium containing Merkel cells. From climax stage C to D, Merkel cells extend cytoplasmic processes along the basal lamina toward the center of the newly-forming fungiform papillae. The morphology of these Merkel cells exactly coincides with that of serotonergic basal cells in adult taste organs. Profuse exocytotic release of dense-cored granules of Merkel cells toward the nerve fibers through the basal lamina is frequently seen in these stages. The present study indicates that serotonergic basal cells are derived from intraepithelial Merkel cells, which act as target sites for growing nerves and might be responsible for the initiation of taste organ morphogenesis.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Toyoshima,K.,Takeda,S.et al: "Early innervation of taste bud-bearing epithelium in the developing circumvallate papilla of the rat." (準備中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyoshima,K.,Seta,Y.et al: "Embryonic development of taste buds in the foliate popillae of the rabbit." (準備中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyoshima,K.,Taked,S.et al: "Regeneration of taste buds of the rabbit foliate papillae after bilateral denervation of glossopharyngeal nerres." (準備中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyoshima,K.,Seta,Y.et al: "Merkel cells are responsible for the initiation of taste organ morphogenesis in the frog." J.Comp.Neurol. 406. 129-140 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seta,Y.,Toyoshima,K.et al: "Expression of Mash 1 in basal cells of rat circumvallate taste budsis dependent upon gustatory innervation." FEBS Lett.444. 43-46 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hamasuki,K.,Toyoshima,K et al: "Possible role of serotonin in Merkel-like basal cells of the taste buds of the frog,Rana nigromaculata." J.Anat. 193. 599-610 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 豊島邦昭、他(共著): "Ten Cate 口腔組織学(訳)" 医歯薬出版社, 632 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 豊島邦昭、他(共著): "味覚の科学" 朝倉書店(印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyoshima, K., Takeda, S, Seta, Y.and Toyosno, T.: "Early innervation of taste bud-bearing epithelium in the developing circumvallate papilla of the rat" (in preparing).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyoshima, K., Seta, Y., Takeda, S.and Toyono, T.: "Embryonic development of taste buds in the foliate papillae of the rabbit" (in priparing).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyoshima, K., Taketa, S., Seta, Y.and Toyono, T.: "Regeneration of taste buds of the rabbit foliate papillae after bilateral denervation of glossopharyngeal nerves" (in preparing).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyoshima, K., Seta, Y., Toyono, T.and Takeda, S.: "Merkel cells are responsible for the initiation of taste organ morphogenesis in the frog" J.Comp.Neurol.406. 129-140 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seta, Y., Toyono, T., Takada, S.and Toyoshima, K.: "Expression of Mash 1 in basal cells of rat circumvallate taste buds is dependent upon gustatory innervation" FEBS Lett.444. 43-46 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hamasaki, K., Seta, Y., Yamada, K.and Toyoshima, K.: "Possible role of serotonin in Merkel-like basal cells of the taste buds of the frog, Rana nigromaculata." J.Anat.193. 599-610 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyoshima, K., Seta, Y., Takeda, S.and Harada, H.: "Identification of Merkel cells by an antibody to villin." J.Histochem.Cytochem.46. 1329-1334 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada, H., Mitsuyasu, T., Seta, Y., Maruoka, Y., Toyoshima, K.and Yasumoto, S.: "Overexpression of bcl-2 protein inhibits terminal differentiation of oral keratinocytes in vitro." J.Oral Pathol.Med.27. 11-17 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada, H., Mitsuyasu, T., Nakamura, N., Higuchi, Y., Toyoshima, K., Taniguchi, A.and Yasumoto, S.: "Establishment of ameloblastoma cell line, AM-1." J.Oral Pathol.Med.27. 207-212 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tandler, B., Nagato, T., Toyoshima, K.and Phillips, C.J.: "Comparative ultrastructure of intercalated ducts in major salivary glands : a review." Anat.Rec.252. 64-91 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tandler, B., Nagato, T., Toyoshima, K.and Phillips, C.J.: "Ultrastructure of the parotid gland in the common vampire bat, Desmodus rotundus, with spacial emphasis on oncocytes." J.Submicrosc.Cytol.Pathol.29. 37-49 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mitsuyasu, T., Harada, H., Higuchi, Y., Kimura, K., Nakamura, N., Katsuki, T., Kubota, E., Toyoshima, K.and Ohishi, M.: "Immunohistochemical demonstration of bcl-protein in ameloblastoma." J.Oral Pathol.Med.26. 345-348 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tandler, B., Toyoshima, K., Seta, Y.and Phillips, C.J.: "Ultrastructure of the salivary glands in the midtongue of the common vampire bat, Desmodus rotundus." Anat.Rec.249. 196-205 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maruoka, Y., Harada, H., Mitsuyasu, T., Seta, Y., Kurokawa, H., Kajiyama, M.and Toyoshima, K.: "Keratinocytes become terminally differentiated in a process involving programmed cell death." Biochem.Biophys.Res.Comm.238. 886-890 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seta, Y., Harada, H.and Toyoshima, K.: "Isolation, partial purification, and ultrastructure of taste bud cells from rabbit foliate papillae." Neurosci.Lett.227. 61-64 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seta,Y: "Isolation,partial purification,and ultrastructure of taste bud cells from rabbit foliate papilke" Neurosci,Lett.227. 61-64 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Harada,H.: "Over expression of bcl-2 protein inhibits terminal differentiation of oral keratinocytes in vito." J.Oral Pathol.Med.27. 11-17 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Maruoka,Y: "Kerationocytes become terminally differentiated in a process involving programmed cell death." Biochem.Biophys.Res.Comm.238. 886-890 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tandler,B.: "Ultrastructure of the salivary glands in the midtongue of the common vampire bat,Desmodus rotundus." Anat.Rec.246. 196-205 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tandler,B.: "Ultrastructure of the parotidl gland in the common vampire bat,with special emphasis on oncocytes," J.Submicrosc.Cytol.Pathol.29. 37-49 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 豊島邦昭: "最新 味覚の科学" 佐藤昌康・小川尚(編集) 朝倉書店, 251 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tandler,B.,Toyoshima,K,et. al.: "Ultrastructure of the salivary glamds in the mid-tongue-" Anat.Rec.(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tandler,B.,Nagato,T.Toyoshima,K.,et al.: "Ultrastructure of the parotid gland in the common vampire bat." J.Submicrosc.Cytol.Pathol.29(1). 37-49 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tandler,B.,Toyoshima,K.et al.: "Symbiotic bacteria in the accessory submandibular gland of the club-footed bat." Ann.Anat.177. 111-117 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 豊島邦昭,他(共著): "Ten Cate口腔組織学(訳)" 医歯薬出版社, 632 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 豊島邦昭,他(共著): "味覚の科学" 朝倉書店(印刷中), (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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