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An analysis of the roles of several kinds of growth factors and their interaction in the morphogenesis of the oral mucosa

Research Project

Project/Area Number 08672102
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

IWASAKI Shin-ichi  The Nippon Dental University Schhol of Dentistry at Niigata, Department of Histology, Lecturer, 新潟歯学部, 講師 (70147833)

Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
KeywordsLingual mucosa / Morphogenesis / Growth fuctors / Keratin subunit / Immunohistochemistry / Ployclonal antibody / Monoclonal antibody / Fetus
Research Abstract

The present study has been designed to analyze of the roles of several kinds of growth factors and their interaction in the morphogenesis of the oral mucosa. For this purpose..two different methods have been employed ; comparative ultrastructural observations of the lingual epithelium in amphibians, reptiles avians and mammals by electron microscopy, and immunohistochemistry of antibody of growth factors and keratin subunits in the lingual mucosa in the rodent.
The results obtained as follows ;
1. Scanning electron microscopic observations of the tongue of the rat and the mouse showed that the period of formation of the lingual papillae was different based on the type of poillae. The rudiments of the fungiform and circuavallate papillae were recognized prior to formation of those of filiform papillae. These facts indicated that the mechanism of morphogenesis was different between papillae with taste buds ant those without them. Keratinization of the lingual epithelium progressed with the … More advance of morphogenesis of filiform papillae.
2. Light and transmission electron microscopic observations revealed that keratinization of filiform papillae started just before birth. This phenomena was supposed as a adaptive change of the dorsal epithelium of the tongue of the juvenile to the dry circumstance.
3. Light and transmission electron microscopic observations revealed that the rudiments of the fungiform papillae were formed by the depression of the epithelium into the deep area in the connective tissue prior to the approachin of the sensory nerve to the epithelum. These rudiments moved to the dorsal epithelium from both sides of the median sulcus to the lateralsides. The differenciation of taste buds would begin at the approaching of sensory nerve to the rudimets of fungiform papillae.
4. Immunohistochemical observations of keratin subunits showed the negative reaction of the dorsal epithelium of the tongue of the fetuses of rats and mice agaist both polyclonal and monoclonal antibody, and the relatively similar results between rats and mice in the postnatal development. Nmanely, immunohistochemical reaction of polyclonal antibody of keratin antibody were widely recognizable in the lingual epithelium of both species.
5. Immunohistochemical reaction of epidarmal growth factors (EGF, FGF, TGF, NGF) in the lingual epithelium was different between rats and mice. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Shin-ichi Iwasaki et al.: "Fine structure of the dorsal lingual epithelium of the juvenile Hawksbill turtle, Eretmochelys imbricata bissa." The Anatomical Record. 244(4). 437-443 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the Asian snail-eating turtle, Malayemys subtrijuga." Annals of Anatomy. 178(2). 145-152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Three-dimensional ultrastructure of the surface of the tongue of the rat snake, Elaphe climacophora." The Anatomical Record. 245(1). 9-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the lingual epitheluim of the juvenile Pacific ridley turtle, Lepidochelys olivacea (Chelonia. Cheloniidae)." Annals of Anatomy. 178(3). 243-250 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the soft-shell turtle, Trionyx cartilagineus" The Anatomical Record. 246(3). 305-316 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the mouse" Acta Anatomica. 157(1). 41-52 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the keratinization of the dorsal epithelium of the tongue of Middendorff′s bean-goose, Anser fabalis middendorffil(Anseres, Anatidae)" The Anatomical Record. 247(2). 149-163 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the rat" The Anatomical Record. 247(4). 528-541 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the effects of cholinergic and adrenergic agonists on salivary secretion from the lingual epithelium and the lingual gland of the Tokyo Daruma pond frog" Tissue & Cell. 29(3). 323-338 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the effects of tachykinins on protein secretion from thelingual epithelium and the lingual gland of the Tokyo daruma pond frog (Rana prosa porosa)" Archive of Oral Biology. 43(6). 463-471 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the relationship between the morphogenesis of filiform papillae and the keratinization of the lingual epithelium in the rat" Journal of Anatomy. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the relationship between the morphogenesis of filiform papillae and the keratinization of the lingual epithelium in the mouse" Cells Tissues Organs. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Fine structure of the dorsal ling epithelium of the juvenile Hawksbill turtle, Eretmochelys imbricata bissa." The Anatomical Record. 44(4). 437-443 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the Asian snail-eating turtle, Malayemys subtrijuga." Annals of Anatomy. 178(2). 145-152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Three-dimensional ultrastructure of the surface of the tongue of the rat snake, Elaphe climacophora." The Anatomical Record. 245(1). 9-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Histoligical and ultrastructural study of the lingual epitheluim of the juvenile Pacific ridley turtle, Lepidochelys olivacea (chelonia, Cheloniidae)." Annals of Anatomy. 178(3). 243-250 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the soft-shell turtle, Trionyx cartilagineus." The Anatomical Record. 246(3). 305-316 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the mouse." Acta Anatomica. 157(1). 41-52 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Jltrastructural study of the kerationozation of the dorsal epithelium of tongue of Middendorff's bean-goose, Anser fabalis middendorffii (Anseres, Anatide)" The Anatomical Record. 247(2). 149-163 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types types of lingual in the rat." The Anatomical Record. 247(4). 528-541 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the effects of cholinergic and adrenergic agonists on salivary secretion from the lingual epithelium and the lingual gland of the Tokyo Daruma pond frog." Tissue & Cell. 29(3). 323-338 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the effects of tachykinis on protein secretion from the lingual epithelium and the lingual gland of the Tokyo daruma pond frog (Rana porosa porosa)." Archives of Oral Biology. 43(6). 463-471 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the relashionship between the morphogenesis of filiform papillae and keratinozation of the lingual epithelium in the rat." Journal of Anatomy. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the relashionship between the morphogenesis of filiform paillae and keratinozation of the lingual epithelium in the mouse." Cells Tissues Organs. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shin-ichi Iwasaki et al.: "Fine structure of the dorsal lingual epithelium of the juvenile Hawksbill turtle, Eretmochelys imbricata bissa." The Anatomical Record. 244(4). 437-443 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the lingual epithelium of the juvenile Pacific ridley turtle, Lepidochelys olivacea(Chelonia, Cheloniidae)" Annals of Anatomy. 178(3). 243-250 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the soft-shell turtle, Trionyx cartilagineus" The Anatomical Record. 246(3). 305-316 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the mouse" Acta Anatomica. 157(1). 41-52 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the keratinization of the dorsal epithelium of the tongue of Middendorff's bean-goose, Anser fabalis middendorffii(Anseres, Anatidae)" The Anatomical Record. 247(2). 149-163 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the rat" The Anatomical Record. 247(4). 528-541 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the effects of tachykinins on protein secretion from thelingual epithelium and the lingual gland of the Tokyo daruma pond frog(Rana prosa porosa)" Archive of Oral Biology. 43(6). 463-471 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the relationship between the morphogenesis of filiform papillae and the keratinization of the lingual epithelium in the rat" Journal of Anatomy. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the relationship between the morphogenesis of filiform papillae and the keratinization of the lingual epithelium in the mouse" Cells Tissues Organs. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Fine structure of the dorsal lingual epithelium of the juvenile Hawksbill turtle,Eretmochelys imbricata bissa." The anatomical record. 244(4). 437-443 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the Asian anail-eating turtle,Malayemys subtrijuga." Annals of Anatomy. 178(2). 145-152 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Three-dimensional ultrastructure of the surface of the tongue of the rat snake,Elaphe climacophora." The Anatomical Record. 245(1). 9-12 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the lingual epitheluim of the juvenile Pacific ridley turtle,Lepidochelys olivacea(Chelonia,Cheloniidae)." Annals of Anatomy. 178(3). 243-250 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the soft-shell turtle,Trionyx cartilagineus" The Anatomical Record. 246(3). 305-316 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the mouse" Acta Anatomica. 157(1). 41-52 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the keratinization of the dorsal epithelium of the tongue of Middendorff´s bean-goose,Anser fabalis middendorffii(Anseres,Antidae)" The Anatomical Record. 247(2). 149-163 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the rat" The Anatomical Record. 247(4). 528-541 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the effects of cholinergic and adrenergic agonists onsalivary secretion from the lingual epithelium and the lingual gland of the Tokyo Daruma pond frog" Tissue & Cell. 29(3). 323-338 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Fine structure of the dorsal lingual epithelium of the juvenile Hawksbill turtle,Eretmochelys imbricata bissa." The Anatomical Record. 244(4). 437-443 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the Asian snail-eating turtle,Malayemys subtrijuga." Annals of Anatomy. 178(2). 145-152 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Three-dimensional ultrastructure of the surface of the tongue of the rat snake,Elaphe climacophora." The Anatomical Record. 245(1). 9-12 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Histological and ultrastructural study of the lingual epitheluim of the juvenile Pacific ridley turtle,Lepidochelys olivacea (Chelonia,Cheloniidae)." Annals of Anatomy. 178(3). 243-250 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the dorsal lingual epithelium of the soft-shell turtle,Trionyx cartilagineus" The Anatomical Record. 246(3). 305-316 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Ultrastructural study of the keratinization of the dorsal epithelium of the tongue of Middendorff's bean-goose,Anser fabalis middendorffii (Anseres,Antidae)" The Anatomical Record. 247(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the mouse" Acta Anatomica. 156(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shin-ichi Iwasaki et al.: "Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the rat" The Anatomical Record. 248(in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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