| Project/Area Number |
08672102
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
IWASAKI Shin-ichi The Nippon Dental University Schhol of Dentistry at Niigata, Department of Histology, Lecturer, 新潟歯学部, 講師 (70147833)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Lingual mucosa / Morphogenesis / Growth fuctors / Keratin subunit / Immunohistochemistry / Ployclonal antibody / Monoclonal antibody / Fetus |
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| Research Abstract |
The present study has been designed to analyze of the roles of several kinds of growth factors and their interaction in the morphogenesis of the oral mucosa. For this purpose..two different methods have been employed ; comparative ultrastructural observations of the lingual epithelium in amphibians, reptiles avians and mammals by electron microscopy, and immunohistochemistry of antibody of growth factors and keratin subunits in the lingual mucosa in the rodent.
The results obtained as follows ;
1. Scanning electron microscopic observations of the tongue of the rat and the mouse showed that the period of formation of the lingual papillae was different based on the type of poillae. The rudiments of the fungiform and circuavallate papillae were recognized prior to formation of those of filiform papillae. These facts indicated that the mechanism of morphogenesis was different between papillae with taste buds ant those without them. Keratinization of the lingual epithelium progressed with the
… More
advance of morphogenesis of filiform papillae.
2. Light and transmission electron microscopic observations revealed that keratinization of filiform papillae started just before birth. This phenomena was supposed as a adaptive change of the dorsal epithelium of the tongue of the juvenile to the dry circumstance.
3. Light and transmission electron microscopic observations revealed that the rudiments of the fungiform papillae were formed by the depression of the epithelium into the deep area in the connective tissue prior to the approachin of the sensory nerve to the epithelum. These rudiments moved to the dorsal epithelium from both sides of the median sulcus to the lateralsides. The differenciation of taste buds would begin at the approaching of sensory nerve to the rudimets of fungiform papillae.
4. Immunohistochemical observations of keratin subunits showed the negative reaction of the dorsal epithelium of the tongue of the fetuses of rats and mice agaist both polyclonal and monoclonal antibody, and the relatively similar results between rats and mice in the postnatal development. Nmanely, immunohistochemical reaction of polyclonal antibody of keratin antibody were widely recognizable in the lingual epithelium of both species.
5. Immunohistochemical reaction of epidarmal growth factors (EGF, FGF, TGF, NGF) in the lingual epithelium was different between rats and mice. Less
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