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Immunocytochemical study on the mechanisms of phagocytotic elimination of apoptotic ameloblasts in rat incisors

Research Project

Project/Area Number 08672107
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionTsurumi University

Principal Investigator

NISHIKAWA Sumio  Tsurumi University School of Dental Medicine, Derpartment of Biology, Lecturer, 歯学部, 講師 (60097277)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsAmeloblasts / Apoptosis / Phagocytosis / Immunohistochemistry / Cell nucleus / 貧食機構
Research Abstract

The head investigator of this research project has previously reported on the apoptosis of ameloblasts in rat incisors. Based on results that showed that ameloblasts in the transitional zone between secretion and maturation die in part via apoptosis, it is shown in this study that fragmented apoptotic ameloblasts are eliminated by the phagocytotic activity of neighboring ameloblasts, epithelial cells in the papillary layr, and macrophage-like cells. The macrophage-like cells invade the papillary layr in the transitional zone, and express MHC class II antigen at their plasma membrane. Thus, it is possible that the macrophages ingesting apoptotic cell fragments present self-antigens derived from phagocytosed ameloblasts. Throughout apoptosis, the relocation or disappearance of U class snRNA serving pre-mRNA processing and several RNA-related proteins occurs in the nucleus, in addition to well-known DNA relocation. Ameloblasts are culled by apoptosis in the transition stage, probably because a large number of ameloblasts are no longer necessary to mature enamel matrix. It will be necessary to investigate whether or not apoptosis occurs in diverse tooth-related tissues and what triggers tooth apoptosis

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Nishikawa, Sumio: "Phagocytotic processing of apoptotic bodies of transitional ameloblasts by MHC class II-expressing macrophages in rat incisor" Journal of Histochemistry and Cytochemistry. Vol.44 No.12. 1459-1467

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishikawa, Sumio: "Apoptosis(programmed cell death)of transitional ameloblasts and MHC class II expression in rat incisors" Acta Anatomica Nipponica. Vol.71 No.6. 597-603

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishikawa, Sumio: "Localization of 2,2,7-trimethylguanosine,SC-35 and TIAR in ameloblast nuclei of the rat incisor,and their disappearance during apoptosis" Acta Histochem Cytochem. Vol.31 No.2 (in press.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishikawa, Sumio: "Phagocytotic processing of apoptotic bodies of transitional ameloblasts by MHC class II-expressing macrophages in rat incisor" J.Histochem.Cytochem.Vol.44. 1459-1467 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishikawa, Sumio: "Apoptosis (programd cell death) of the transitional ameloblasts and MHC class II expression in rat incisors" Acta Anat.Nippon.Vol.71. 597-603 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishikawa, Sumio: "Localization of 2,2,7-trimethylguanosine, SC-35 and TIAR in ameloblast nuclei of the rat incisor, and their disappearance during apoptosis" Acta Histochem.Cytochem.Vol.31, (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishikawa,Sumio: "Phagocytotic processing of apoptotic bodies of transitional ameloblasts by MHC class II-expressing macrophages in rat incisor" Journal of Histochemistry and Cytochemistry. Vol.44 No.12. 1459-1467 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nishikawa,Sumio: "Apoptosis(programmed cell death)of transitional ameloblasts and MHC class II expression in rat incisors" Acta Anatomica Nipponica. Vol.71 No.6. 597-603 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nishikawa,Sumio: "Localization of 2,2,7-trimethylguanosine,SC-35 and TIAR in ameloblast nuclei of the rat incisor,and their disappearance during apoptosis" Acta Histochem Cytochem. Vol.31 No.2(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nishikawa,Sumio: "Phagocytotic processing of apoptotic bodies of transitional ameloblasts by MHC class II-expressing macrophages in rat incisor" Journal of Histochemistry and Cytochemistry. Vol.44 No.12. 1459-1467 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishikawa,Sumio: "Apoptosis (programmed cell death) of transitional ameloblasts and MHC class II expression in rat incisors" Acta Anatomica Nipponica. Vol.71 No.6. 597-603 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishikawa,Sumio: "Internalization of styryl dye FM 1-43 in the hair cells of lateral line organs in Xenopus larvae" Journal of Histochemistry and Cytochemistry. Vol.44 No.7. 733-741 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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