PARASYMPATHETIC VASODILATOR RESPONSE IN THE OROFACIAL REGION MODULATED BY STIMULATION OF THE HIGHER CENTER.

• Project/Area Number: 08672117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: TOHOKU UNIVERSITY
• Principal Investigator: KARITA Keishiro TOHOKU UNIV., SCHOOL OF DENTISTRY,ASSOC.PROF., 歯学部, 助教授 (40004600)
• Project Period (FY): 1996 – 1997
• Project Status: Completed (Fiscal Year 1997)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: parasympathetic nerve / vasodilatation / hypothalamus / periaquiductal gray / cat / ネコ / 中脳灰白質 / 副交感神経性血管拡張反射 / 抑制効果

Research Abstract

The present study investigated the modulation by the anterior hypothalamus (AH) and the periaqueductal gray matter (PAG) of the parasympathetically mediated reflex vasodilatation in lower lip and palate evoked by electrical stimulation of the lingual nerve (LN) in urethane-chloralose-anesthetized and vagosympathectomized cats. Electrical stimulation of the AH and PAG at intensities that did not elicit a pressor effect consistently elicited an intensity (50-500 mA)-dependent attenuation of the lip blood flow increase evoked reflexly by LN stimulation. The optimal stimulus frequency for the inhibitory effect was 50 Hz. The greatest inhibitory effects were evoked from the periventricular region of the anterior hypothalamic area and the PAG at sites in areas A0.5-A2.5. Prior administration of picrotoxin, a relatively specific antagonist of gamma-aminobutyric acid (GABA) receptors, at a dose of 1 mg/kg (i.v.), abolished the inhibitory effect of anterior hypothalamic stimulation completely at any of the stimulus intensities and frequencies used, suggesting that the inhibitory effect of hypothalamic stimulation might be exerted via a GABA-like process effect. Microinjection into the AH of D,L-homocysteic acid (1 M,0.2 ml), an excitatory amino acid, inhibited the lip blood flow increase elicited reflexly by LN stimulation singnificantly. These results suggest that the AH and the PAG are involved in the inhibitory action on the somato-parasympathtic vasodilator reflex in the oro-facial area.

Research Products

• [Publications] 刈田 啓史郎: "口腔顔面領域における副交感神経性血管拡張反射" 東北大学歯学雑誌. 16(2). 87-96 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sasano, T. et al.: "Direct evidence of parasympathetic vasadilatation in cat periodontal ligament." J.Periodontal Res.31. 556-562 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yosui, T. et al.: "Correlation between vasodilatation and secretion in the lacrimal gland elicited by stimulation of the corbea and faeial herve root of the cat" Invest.Ophthalmol.,and Visual Sci.38(2). 2476-2482 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sato, M. et al.: "Comparative effects of lingual and facial nerve stimulation on intraoranial and extracranisl vaso motor pesponses in anesthetized cat" Tohoku J.Exp.Med.182. 103-113 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Izumi, H. et al.: "Effect of inhalation anesttetics on the parasympathetic reflex vasedilatation in the lower lip and palate of the cat." Amer.J.Physiol.273. R168-R174 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Izumi, H. et al.: "Blood flow inveases in common carotid artery,lower lip and palate of the cat." J.Auton.Nerv.Sys.62. 167-173 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Karita, K.: "Parasympathetic vasodilator reflex in the orofacial region." Tohoku Univ.Dent.J. 16 (2). 87-96 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sato, M., Izumi, H., Karita, K.and Iwatsuki, N.: "Comparative effects of lingual and facial nerve stimulation on intracranial and extracranial vasomotor tesponses in anesthetized cats." Tohoku J.exp.Med.182. 103-113 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yasui, T., Karita, K., Izumi, H.and Tamai, M.: "Correlation between vasodilatation and secretion in the lacrimal gland elicited by stimulation of the cornea and facial nerve root of the cat." Invest.Ophthalmol.Visual Science.38. 2476-2482 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Izumi, H,Ito, Y,Sato, M., Karita, K.and Iwatsuki, N.: "Effect of inhalation anesthetics on the parasympathetic reflex vasodilatation in the lower lip and palate of the cat." Amer.J.Physiol.273. R168-R174 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Izumi, H., Sato, M., Karita, K.and Iwatsuki, H.: "Blood flow increases in common carotid artery, lower lip and palate elicited by lingual nerve stimulation in anesthetized cat." J.Autonomic Nervous System. 62. 167-173 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sasano, T., Shoji, N., Kuriwada, S., Sanjo, D., Izumi, H.and Karita, K.: "Direct evidence of parasympathetic vasodilatation in cat periodontal ligament." J.Periodontal Res.31. 556-562 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 刈田啓史郎: "口腔・顔面領域における副交感神経性血管拡張反射" 東北大学歯学雑誌. 16・2. 87-96 (1997)
• [Publications] Yasui,T.et al.: "Correlation between vasodilatation and seretion in the locrimal gland elicited by stimutation of the cornea and tocial nerve root of the cat" Invest.Ophthalmol. and Visual Sci.38・2. 2476-2482 (1997)
• [Publications] Izumi,H.et al.: "Blood flow inveases in common carotid artery,lower lip and palate elicited by lingual nerve stimulation in anesthetized cats" J.Automomic Nervous System. 62. 167-173 (1997)
• [Publications] Sato,M.et al.: "Comparative effects of lingual and facial herve stimulation on intracranial and extracranial vasomotor responses in anesthetized cats" Tohoku J.Exp.Med.182. 103-113 (1997)
• [Publications] Izumi,H.et al: "Effects of inhalation anesthetics on parasympathetic reflex vasodilatation in the lower lip and palate of the cat." Amer.J.Physiol.273. R168-R174 (1997)
• [Publications] Sasano, T.: "Direct evidence of parasy mpathetic vasodilatation in cat pnodontal ligament." J. Periodontal Rec.31. 556-562 (1996)
• [Publications] Izumi, H.: "Effect of inhalation anesthetics on the parasy mpathetic reflex vasodilatation in the lower lip and palate of the cat." Amer J. Physiol.(in press). (1997)
• [Publications] Izumi, H.: "Blood flow increases in common carotid artery, lower lip and palate elicited by lingual nerve stimulation in anesthetized cat." J. Auton. Nervous Syst.62. 167-173 (1997)
• [Publications] Yasui T.: "Correlation between vasodilatation and secretion in the lacrimal gland elicited by stimulation of the comea and facial nerve root of the cat." Inrestigative Opthalmology, Visual Suience. (in press). (1997)