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Molecular-Pharmacological Analysis of Periodontal Disease : Interaction between LPS and Kinins on Microcirculation of Oral Region

Research Project

Project/Area Number 08672142
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

TODOKI Kazuo  Assistant Professor, Kanagawa Dental College, Pharmacology, 歯学部, 講師 (90139577)

Co-Investigator(Kenkyū-buntansha) SHOJI Hirofumi  Instructor, Kanagawa Dental College, 歯学部, 助手 (90277913)
LEE Chang-il  Instructor, Kanagawa Dental College, 歯学部, 助手 (60220795)
TAKAHASHI Syun-suke  Instructor, Kanagawa Dental College, 歯学部, 助手 (60206810)
OKABE Eiichiro  Professor, Kanagawa Dental College, Pharmacology, 歯学部, 教授 (50097276)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsOptical biosensor / Hypoxanthine / Ischemia / Chemiluminescence / Hydrogen peroxide / Luminol / Oxygen free radicals / Plasuma / フリーラジカル
Research Abstract

The purpose of this study is twofold : 1) to design and develop an optical biosensor that can be used without renewing reagents in the probe for detection of biologically important analytes, and 2) to apply the hypoxanthine affinity optical biosensor to measuring the purine metabolism in an ischemia-reperfusion models. We developed the chemiluminometric bienzyme sensors on the basis of the horseradish peroxidase catalyzed H_2O_2/luminol reaction ; horseradish peroxidase was coimmobilized with xanthine oxidase. Optical biosensor was developed for the batch analysis system of hypoxanthine in the concentration range between 10^<-8> and 10^<-4> M.Optical biosensor probe with photomultiplier tube (PMP) with an integrated preamplifier was investigated to detect the chemiluminescence (which is enhanced by horseradish peroxidase). Horseradish peroxidase and xanthine oxidase were immobilized entrapping sodium alginate gel. The probe based superoxide anion radicals or H_2O_2 sensor achieve detection limits 10^<-8> M.The concentration of hypoxanthine in rabbit serum was also determined in peripheral blood samples (obtained from auricular vein). Increase in hypoxanthine levels was observed in serum obtained immediately after 90 min of auricular artery occlusion. Although there is conflicting evidence as to whether oxygen-derived free radicals play a major role in reperfusion injury, the relatively sustained nature of enhanced oxygen-derived free radical generation during reperfusion has potential clinical implications. With this optical biosensor, it should be possible to measure oxygen-derived free radical generation in a variety of conditions and with multiple interventions. Such studies may further characterize the role of oxygen free radicals in ischemiaミreperfusion injury.

Report

(2 results)
  • 1997 Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 塗々木 和男: "生体フリーラジカル検出とその展望" 日本薬理学雑誌. 108・6. 295-306 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 塗々木 和男: "神経ペプチドCGRP誘発性血管拡張に対するfree radicalsの作用様式" 磁気共鳴と医学. 7・1. 85-88 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 和田 聡子: "血管平滑筋脱膜標本収縮反応性に対するフリーラジカルの効果" 磁気共鳴と医学. 7・1. 93-96 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Katsufumi Nanba: "Vascular action of bradykinin is mediated by B_2-subtype receptors in the canine lingual artery" Japanese Journal of Oral Biology. 38・2. 173-181 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masahiro Norisue: "Inhibiton by hydeoxyl radicals of calcitonin gene- related peptide-mediated neurogenic vasorelaxation in isolated canine lingual artery" Journal of Pharmacology and Experimental Therapeutics. 280・1. 492-500 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岡部 栄逸朗: "NOラジカルの医学" 羊土社, 12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuo Todoki, Chang-il Lee, Eiichiro Okabe: "Measurement of reactive oxygen species in a biological system and its perspectives." Folia Pharmacol.Jpn.108. 295-306 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Eiichiro Okabe, Kazuo Todoki, Chang-il Lee, Tetsuya Hagiwara and Hirofumi Shoji: "Reactive oxygen species in apoptosis : redox regulation of programd cell death." Bulletin of Kanagawa Dental College. 24. 97-106 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satoko Wada, Masanori Kiyose, Satoru Kumasaka, Midori Kawakami, Kazuo Todoki, Eiichiro Okabe: "Effevt of reactive oxygen species on reactivity of a-toxin-permeabilized rabbit mesenteric artery." Magnetic Resonance in Medicine. 7. 93-96 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuo Todoki, Dai Kobayashi, Shinobu Kuwaki, Takashi Kiyose, Midori Kawakami, Eiichiro Okabe: "Mode of action of Hydroxyl radical underlying depressed CGRP-mediated relaxation of canine lingual artery." Magnetic Resonance in Medicine. 7. 85-88 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masahiro Norisue, Kazuo Todoki and Eiichiro Okabe: "Inhibition by hydroxyl radicals of calcitonin gene-related peptide-mediated neurogenic vasorelaxation in isolated canine lingual artery." J.Pharmacol.Exp.Ther.280. 492-500 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Katsufumi Nanba, Kazuo Todoki and Eiichiro Okabe: "Vascular action of bradykinin is mediated by B2-subtype receptors in the canine lingual artery." Jpn.J.Oral.Biol.38. 178-181 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Eiichiro Okabe, Kazuo Todoki: Cardiovascular system and NO radical. In NO Radicals in Medicine, ed. Oyanagui, Y.Yoda-sha, Tokyo, 71-81 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 塗々木和男: "生体フリーラジカル検出とその展望" 日本薬理学雑誌. 108・6. 295-306 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 塗々木和男: "神経ペプチドCGRP誘発性血管拡張に対するfree radicalsの作用様式" 磁気共鳴と医学. 7・1. 85-88 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Katsufumi Nanba: "Vascular action of bradykinin is mediated by B_2-subtype receptors in the canine lingual artery" Japanese Journal of Oral Biology. 38・2. 173-181 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuo Todoki: "Inhibitory effect of oxygen free radicals on CGRP-mediated vasorelaxation" Microcirculation annual. 11・1. 37-38 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 塗々木和男: "オプティカルバイオセンサー:虚血再灌流への応用" 磁気共鳴と医学. 8・1. 193-196 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masahiro Norisue: "Inhibition by hydroxyl radicals of calcitonin gene-related peptide-mediated neurogenic vasorelaxation in isolated canine lingual artery" Journal of Pharmacology and Experimental Therapeutics. 280・1. 492-500 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岡部栄逸朗: "NOラジカルの医学" 羊土社, 12 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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