| Project/Area Number |
08672145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | TSURUMI UNIVERSITY |
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Principal Investigator |
SHIMIZU Masaharu SCHOOL OF DENTAL MEDICINE,DEPARTMENT OF BIOCHEMISTRY,PROFESSOR, 歯学部, 教授 (40064357)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMOTO Tadafumi SCHOOL 0F DENTAL MEDICINE,DEPARTMENT OF BIOCHEMISTRY,ASSISTANT PROFESSOR, 歯学部, 助手 (60148046)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | AMELOGENESIS / ENAMEL / CALCIUM TRANSPORT / RAT / AUTORADIOGRAPHY |
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| Research Abstract |
The pathway of calcium movement to mineralizing enamel was studied by ^<45>Ca autoradiography. Freeze-dried lower incisor section of rats were frozen 4.3,6.1,7.8,10.6,13.7 sec after an i.v.injection were prepared, and the light microscopical autoradiogrpahy was carried out by the previously described method (J.Histochem.Cytochem.38 : 1805-1814,1990)
The autoradiograms showed that the intercellular junctions of the proximal side of the smooth-ended ameloblast (SA) and the distal side of the ruffle-ended ameloblast (RA) were closed to calcium. The junctions of the distal side of SA,the proximal side of RA,and both sides of the secretory stage ameloblasts were not. The time required for calcium to pass through the ameloblast layr was less than 1.8 sec in the secretory stage and SA region. The time in the RA region was 3.5-6.3 sec. The radioactivity in the secretory stage enamel increased almost linearly with time. The radioactivity in the secretory ameloblast layr was gradually decreased from proximal to distal side.
These results indicate that in the secretory and SA regions calcium moves to the enamel surface through the intercellular spaces of ameloblasts and in the RA region via RA cells.
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