| Project/Area Number |
08672198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
ANAN Hisashi KYUSHU UNIVERSITY DEPT.OF DENTISTRY,ASSISTANT PROFESSOR, 歯学部, 講師 (80158732)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Ryusei KYUSHU UNIVERSITY DEPT.OF DENTISTRY,RESEARCH INSTRUCTOR, 歯学部, 助手 (20205008)
YOSHIMINE Yoshito KYUSHU UNIVERSITY DEPT.OF DENTISTRY,RESEARCH INSTRUCTOR, 歯学部, 助手 (80183705)
橋口 勇 九州大学, 歯学部, 助手 (10150476)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Adhesion molecule / ICAM-1 / LFA-1 / Osteoclast / Macrophage / IL-1 beta / IL-1B / 骨芽細胞 / マイクロファージ / インテグリンβ_3 |
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| Research Abstract |
In order to investigate the pathobiological mechanisms of bone destruction during apical periodontitis, we examined the relationship between the expression of interleukin-1 (IL-1) and that of adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1) and leukocyte function- associated antigen-1 (LFA-1), in rat periapical lesions using immunohistochemical methods. During the acute phase, IL-1-expressing cells were observed adjacent to many osteoclasts which contained large cytoplasms, and consequently the periapical lesions tended to expand. The number of IL-1 beta -expressing cells was about twice that of IL-1 alpha -expressing cells, and the expression of adhesion molecules ICAM-1 and LFA-1 were increased in the vicinity of the bone resorption surfaces during the same period. On the other hand, there were significantly less IL-1 beta -expressing cells during the chronic phase and consequently a significant expansion of periapical lesions was not seen. Furthermore, IL-1 beta -expressing cells were located far from the bone surfaces, and the expression of ICAM-1 and LFA-1 was decreased near the bone surfaces. Both IL-1 alpha and IL-1 beta were mainly expressed by macrophages. These results suggest that macrophages expressing IL-1 beta may play an important role in the activation of osteoclastic bone resorption and this may be due to the increase expression of ICAM-1 and LFA-1 in the vicinity of the bone resorption surfaces within experimental periapical lesions.
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