| Project/Area Number |
08672285
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
MORI Shiro Tohoku University Dental Hospital, Lecturer, 歯学部・附属病院, 講師 (80230069)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | oral squamous cell carcinoma / regional lymph node / micrometastasis / diagnosis / RT-PCR / cytokeratin / tumor marker / radical neck dissection / RT・PCR法 |
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| Research Abstract |
In order to detect micrometastasis of oral squamous cell carcinoma (SCC) in regional lymph nodes, we examined a sensitive method utilizing reverse transcriptase-polymerase chain reaction (RT-PCR) for amplification of cytokeratin 19 (CK-19) mRNA.Expression of CK-19 mRNA was observed in normal oral mucosa and SCCs in primary lesions, but not in any of normal control lymph nodes by use of the RT-PCR.These results indicates that examination of the CK-19 mRNA might be useful in the detection of oral SCC micrometastases in regional lymph nodes. Moreover, sensitivity of the detection of the micrometastases was compared between the CK-19 RT-PCR and conventional histopathological examination using 40 lymph nodes obtained from 6 oral SCC patients which were macroscopically metastasis-free. As a result, CK-19 mRNA was detected in a lymph nodes which were histopathologically metastasis-positive. On the other hand, of the 39 lymph nodes which were histopathologically metastasis-negative, 35 were found not to express CK-19 mRNA but 4 were found to express CK-19 mRNA,indicating that these lymph nodes contained micrometastases which could not be detected by histopathological examination. These results demonstrate that CK-19 RT-PCR is a more sensitive method than histopathological examination for the detection of micrometastases of oral SCC in lymph nodes.
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