Expression of TGF-beta in foreign body reaction and fibrosis agaist titanium implant.
| Project/Area Number |
08672286
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
KATOU Fuminori TOHOKU UNIVERSITY,Dental Hospital Lecturer, 歯学部・附属病院, 講師 (60204492)
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| Co-Investigator(Kenkyū-buntansha) |
OTANI Haru TOHOKU UNIVERSITY,School of Medicine Associate Professor, 歯学部, 助教授 (30133987)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Foreign body reaction / Titunium / Mandible / TGF-beta / 細胞増殖因子 / マクロファージ / 線維芽細胞 |
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| Research Abstract |
The present paper addresses in situ expression of transforming growth factor-beta (TGF-beta), platelet-derived growth factor (PDGF), and their receptors in the encapsulation process against foreign body using 20 cases. We dealt with titanium miniplate as a model of foreign body, which was used clinically to fix fractured mandible. Fibrous capsule formed as the encapsulation process was composed of scar-like-and fibrogenic layrs, with the former directly facing the foreign body. By immunohistochemistry using pre-fixed frozen sections, only the fibrogenic layr was characterized by an expression of type I procollagen (PC-I). Spindle-shaped mesenchymal cells in the fibrogenic layr expressed TGF-beta isoforms and receptors, and PDGF isoforms and PDGF beta-receptor. In contrast, the scar-like layr had few such cells. In both layrs, spindle-shaped cells did not expressed Ki-67 in their nuclei.
Double immunofluorescence microscopy for the cell identification revealed that most PC-I-positive fibroblasts expressed both isoforms and receptors of TGF-beta and PDGF in the fibrogenic layr. In contrast, a small number of CD68-positive macrophages expressed these.
Our results suggest that in the maintenance stage of the human encapsulation process against foreign body, both TGF-beta and PDGF function cooperatively via their receptors in the fibrogenic layr, with such stimuli markedly diminished in the scar-like layr.
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Report
(3 results)
Research Products
(2 results)
