• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis on expression of tumor related antigen and its clinical application in oral cancers.

Research Project

Project/Area Number 08672312
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionYamaguchi University

Principal Investigator

TSUJI Tatsuo  Yamaguchi University hospital, assistant, 医学部附属病院, 助手 (70144954)

Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
KeywordsFISH / p53 / Bcl-2 / chromosome17 / CGH / squamous cell carcinoma / chromosome 3 / chromosome 17
Research Abstract

Numerical aberrations of chromosome 17 was studied by fluorescence in situ hybridization (FISH) using pericentromere specific DNA probe in 27 oral squamous cell carcinoma (SCC), and its relationship with p53 and Bcl-2 protein expression was investigated. Since cells with polysomy 17 significantly increased in SCC (p=0.0005), chromosome 17 abnormality seems to be correlated with carcinogenesis of oral SCC.Chromosome 17 abnormality varied from case to case, and the degree of the abnormality did not correlate with the stage of the disease, the histological differentiation of SCC, relapse of the disease and lymph node matastasis . However, there was the correlation between polysomy 17 and p53 immunoreactivity (p=0.0228). p53 immunoreactivity showed correlation with relapse of the disease (p=0.044 1). An inverse relationship was found between p53 and Bcl-2 immunoreactivity (p=0.042 1). In conclusion, we suggest that polysomy 17 is in close relation to carcinogenesis of oral SCC, and that polysomy 17 significantly correlated with p53 overexpression. The possible mechanism of this phenomenon is assumed that polysomy 17 correlates with mutation of p53 which results in an accumulation of aberrant p53 protein, and that its activation causes an increase of p53 protein.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 辻 龍雄: "口腔癌における染色体異常およびp53遺伝子異常." Cytometry Research. 8・1. 1-5 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akihiro Kanekawa: "Chromosome 17 abnormality in squamous cell carcinoma of the oral cavity, and its relatiohship with p53 and Bcl-12 expression." Anticancer Research. 19・1 掲載予定. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tatsuo Tsuji: "Abnormality of p53 gene and chromosomal aberration in oral cancers." Cytometry Research. 8. 1-5 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akihiro Kanekawa, Sasaki, Fumihiko Shinozaki.: "Chromosome 17 abnormality in squamous cell carcinoma of the oral cavity, and its relatiohship with p53 and Bcl-2 expression." Anticancer Research. 19 (1) (accepted).(1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 辻 龍雄: "口腔癌における染色体異常およびp53遺伝子異常." Cytometry Research. 8・1. 1-5 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akihiro Kanekawa: "Chromosome 17 abnormality in squamous cell carcinoma of the oral cavity,and its relatiohship with p53 and Bcl-2 expression." Anticancer Research. 19・1(掲載予定). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tatsuo Tsuji: "Increased polysomy 3 and 17 detected by fluorescence in situ hybridization (FISH) in oral squamous cell carcinoma." Oncology Reports. 4. 277-280 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shumin Wen: "Detection and analysis of human papillomavirus 16 and 18 homologous DNA sequences in oral lesions." Anticancer Research. 17. 307-312 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomoko Kamada: "Sample preparation from paraffin-embedded tissue specimens for laser scanning cytometric DNA analysis." Cytometry. 27. 290-294 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Xinwei Li: "Detection of numeric abnormalities of chromosome 17 and p53 deletions by fluorescence in situ hybidization in pleomorphic adenomas and carcinomas in pleomorphic adenoma." Cancer. 79. 2314-2319 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kazuyu Ebe: "Novel methods for a simultaneous trimodal therapy for oral cancer consisting of radiotherapy and hyperthermia and intraarterial drug infusion." 日本放射線腫瘍学会誌. 9. 339-344 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 辻 龍雄: "口腔癌における染色体異常・p53遺伝子異常" Cytometry Research. 8. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tatsuo Tsuji: "Oral Oncology Chromosomal aberrations in oral tumours detected by comparative genomic hybridisation." Macmillan India Limited, 600 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tatsuo Tsuji: "Increased polysomy 3 and 17 detected by fluorescence in situ hybridization(FISH)in oral squmous cell carcinoma." Oncology Reports. 4. 277-280 (1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi