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Study of signal transduction of invasion / metastasis via PI3-kinase in the oral squamous cell carcinoma.

Research Project

Project/Area Number 08672325
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

OKUMURA Kazuhiko  Health Sciences University of Hokkaido, 1st Dept.OMS,Assistant prof., 歯学部, 講師 (60194510)

Co-Investigator(Kenkyū-buntansha) KONISHI Atsushi  Health Sciences University of Hokkaido, 1st Dept.OMS,Staff, 歯学部, 助手 (80225466)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Keywordshuman OSCC cells / invasion / metastasis / PI3-kinase / FAK / tyrosine phosphorylation / Cell adhesiveness / Cell mitility / Rho family / 浸潤・転移 / PI3-Kinase / Focal adhesion kinase
Research Abstract

We established different invasiveness clones from human oral squamous cell carcinoma (OSCC) cell line SAS.The factor that defines the invasiveness of the two clones was also examined and two clones showed remarkably different cell motility. Here we report that analyzed signal transduction of cell motility in the OSCC.
The role of phosphatidyinositol 3-kinase (PI3-kinase) activity in serum-stimulated tyrosine phosphorylation of focal adhesion kinase (p125^<FAK>) has been examined. The tyrosine phosdphorylation of p125^<FAK> of high-invasion clone SAS-H1, but not low-invasion clone SAS-L1 in response to serum was markedly inhibited by both wortmannin and LY294002, inhibitor of PI3-kinase in dose dependent manner. Furthermore, wortmannin inhibited adhesiveness to extracellular matrixs and cell motility.
Serum-stimulated PI3-kinase activity, increased invasion of SAS-H1, but not SAS-L1 by endothelial cell monolayr assay.
Thus, we have identified a PI3-kinase dependent signal transduction pathway in the cell motility of high-invasion OSCC cells, as SAS-H1, which leads to the phosphorylation of p125^<FAK>.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Abiko, Y.: "Basaloid-squamous cell carcinoma of the floor of the mouth:characterization of a cell line." J.Oral Pathol.Med.26. 367-370 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 奥村 一彦: "培養ヒト口腔扁平上皮癌細胞の浸潤におけるTransforming growth factor-β1(TGF-β1)のオートクリン作用" 日本口腔外科学会雑誌. 42. 962-968 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okumura, K.: "Establishment of high-and low-invasion clones derived for a human tongue squamous-cell carcinoma cell line SAS" JCancer Res Clin Oncol. 122. 243-748 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 奥村 一彦: "舌扁平上皮癌細胞の浸潤シグナルにおよぼすWortmanninの影響" 口腔組織培養研究会誌. 4. 121-122 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 北所 弘之: "Hepatocyte growth factorによる口腔扁平上皮癌細胞の浸潤シグナルの検討" 口腔組織培養研究会誌. 4. 123-124 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shibata, T.: "Enhancing effects of epidermal growth factor on human squamous cell carcinoma motility and matrix degradation,but not growth." Tumor Biol. 17. 168-175 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Abico, Y., et al.: "Basaloid-squamous cell carcinoma of the floor of the mouth : characterization of a cell line." J Oral Pathol Med. 26. 367-370 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okumura, K., et al.: "Autocrine action of transforming growth factor-beta1 (TGF-beta1) in the invasiveness of human oral squamous cell carcinoma cell lines." Jpn J Oral Maxillofac Surg. 42. 962-968 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okumura, K., et al.: "Establishment of high- and low-invasion clones derived for a human tongue squamous-cell carcinoma cell line SAS." J Cancer Res Clin Oncol. 122. 243-248 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okumura, K., et al.: "Effect of wortmannin of signal transduction pathway of invasion in the tongue squamous cell carcinoma cells." Jpn J Tissue Cult Dent Res. 4. 121-122 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajo, H., et al.: "Study of signal transductin pathway of hepatocye growth factor stimulated invasion in oral squamous cell carcinoma cells." Jpn J Tissue Cult Dent Res. 4. 123-124 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shibata, T., et al.: "Enhancing effects of epidermal growth factor on human squamous cell carcinoma motility and matrix degradation, but not growth." Tumor Biol. 17. 168-175 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Abiko,Y.: "Basaloid-squamous cell carcinoma of the floor of the mouth : characterization of a cell line" J.Oral Pathol.Med.26. 367-370 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 奥村一彦: "培養ヒト口腔扁平上皮癌細胞の浸潤におけるTransforming growth factor-β1(TGF-β1)のオートクリン作用" 日本口腔外科学会雑誌. 42. 962-968 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okumura, K.: "Establishment of high-and low-invasion clones derived for a human tongue squamous-cell carcinoma cell line SAS" J Cancer Res Clin Oncol. 122. 243-748 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 奥村一彦: "舌扁平上皮癌細胞の浸潤シグナルにおよぼすWortmanninの影響" 口腔組織培養研究会誌. 4. 121-122 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 北所弘之: "Hepatocyte growth factorによる口腔扁平上皮癌細胞の浸潤シグナルの検討" 口腔組織培養研究会誌. 4. 123-124 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shibata, T.: "Enhancing effects of epidermal growth factor on human squamous cell carcinome motility and matrix egradation, but not growth" Tumor Biol. 17. 168-175 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 奥村一彦: "舌扁平上皮癌細胞の浸潤シグナルにおよぼすWortmanninの影響" 日本口腔組織培養研究会雑誌. 4・1. 121-122 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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