• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A study on the morphological changes of osteocytes in rat alveolar bone during orthodontic tooth movement

Research Project

Project/Area Number 08672356
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

IGARASHI Kaoru (1997-1998)  Tohoku University, School of Dentistry, Instructor, 歯学部, 助手 (70202851)

賓 広道 (1996)  東北大学, 歯学部, 助手 (50260443)

Co-Investigator(Kenkyū-buntansha) SAEKI Shuichi  Tohoku University, Dental Hospital, Instructor, 歯学部・附属病院, 助手 (60271954)
SASANO Yasuyuki  Tohoku University, School of Dentistry, Instructor, 歯学部, 助手 (30196191)
MIZOGUCHI Itaru  Health Sciences University of Hokkaido, Faculty of Dentistry, Professor, 歯学部, 教授 (20200032)
MITANI Hideo  Tohoku University, School of Dentistry, Professor, 歯学部, 教授 (50014220)
五十嵐 薫  東北大学, 歯学部, 助手 (70202851)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsOsteocyte / Alveolar Bone / Tooth Movement / Intercellular Network / 骨芽細胞 / 歯槽骨細胞 / 免疫組織学 / 遺伝子組織学 / 微細形態学
Research Abstract

The purposes of the present study were 1) to examine the morphological changes of osteocytes in rat alveolar bone during orthodontic tooth movement using immunohistochemistry for actin filaments and 2) to determine a molecular marker for osteocyte metabolism. Eight-week-old male Wistar rats were used in this study. The maxillary first molars were moved buccally by using a expansive spring of Ni-Ti wire with a nominal size of 0.012 inch as described by Igarashi et al. (1998). The animals were killed after 3, 6, 12, 24 hours, 2, 4, 7, 10 and 14 days of tooth movement. After experiments, the animals were fixed with 4 % paraformaldehyde plus 0.5 % glutaraldehyde in 0.1 M PB, pH 7.4, decalcified with 10 % EDTA solution and processed into paraffin embedding. Six-mu in-thick sections were stained with hematoxilin-eosin or antibodies for osteocalcin, typesI,II and X collagen and actin filaments. The results were as follows ;
1) Precursor and mature osteocalcin was a proper molecular marker for examining the metabolic changes of alveolar bone and bone-forming cells.
2) After 3 hours of orthodontic tooth movement, hyaline tissue appeared in the pressure side of the periodontal tissue of all five roots of the first molar. The hyaline tissue spread and almost dessapered at day 10.
3) Immunohistochemistry for actin filament showed intense staining in cytoplasm and cellular processes of osteocytes in control group. Especially young osteocytes showed intense staining. In contrast, the staining of osteocyte cytoplasm adjacent to the hyaline tissue became weak at day 1 and the staining of its cellular processes disappeared at day 4.
These results demonstrated that osteocytes adjacent to the hyaline degeneration tissue became cell death during early stage of orthodontic tooth movement due to the disappearance of intercellular communication between the periodontal tissue cells.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Mizoguchi I.: "Localization of biglycan, decorin and large chondroitin sulfate proteoglycan in adult temporomandibular joint disc." Archives of Oral Biology. 43. 889-898 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sasano Y.: "Type X collagen is not localized in hypertrophic or developing rat trached." Anatomy and Embryology. 197. 399-403 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Igarashi K.: "Diurnal variation in tooth movement in response to orthodontic force in rats." Am.J.Orthod. Dentofacial Orthop.114. 8-14 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi M.: "Localization of anti-monocyte/macrophage antibody-positive cells in periodontal tissue of rat maxillary molars after orthodontic tooth movement." 東日本歯科学会誌. 16. 233-238 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kagayama M.: "Expression of osteocalcin in cementoblasts forming acellular cementum." Journal of Periodontal Research. 32. 273-278 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kagayama M.: "Localization of glycosaminoglycans in periodontal ligament claring physiological and experimental tooth movement." Journal of Periodontal Research. 31. 229-234 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itaru Mizoguchi, et al.: "Localization of biglycan, decorin and large chondroitin sulfate proteoglycan in adult temporomandibular joint disc." Archives of Oral Biology. vol.43. 889-898 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuyuki Sasano, et al.: "Type X collagen is not localized in hypertrophic or developing rat trachea." Anatomy and Embryology. vol.197. 399-403 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kaoru Igarashi, et al.: "Diurnal variation in tooth movement in response to orthodontic force in rats." Am J Orthod Dentofac Orthop. vol.114. 8-14 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Makoto Takeuchi, et al.: "Localization of anti-monocyte/macrophage antibody-positive cells in periodontal tissue of rat maxillary molars after orthodontic tooth movement." Higashi Nippon Dental Journal. vol.16. 233-238 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Manabu Kagayama, et al.: "Expression of osteocalcin in cementoblasts forming acellular cementum." Journal of Periodontal Research. vol.32. 273-278 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Manabu Kagayama, et al.: "Localization of glycosaminoglycans in periodontal ligament during physiological and experimental tooth movement." Journal of Periodontal Research. vol.31. 229-234 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizoguchi I.: "Localization of biglycan, decorin and large chondroitin sulfate proteoglycan in adult temporomandibular joint disc." Archives of Oral Biology. 43. 889-898 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sasano Y.: "Type X collagen is not localized in hypertrophic or developing rat trachea." Anatomy and Embryology. 197. 399-403 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Igarashi K.: "Diurnal variation in tooth movement in response to orthodontic force in rats." Am.J.Orthod, Dentofacial Orthop.114. 8-14 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi M.: "Localization of anti-monocyte/macrophage antibody-positive cells in periodontal tissue of rat maxillary molars after orthodontic tooth movement." 東日本歯科学会誌. 16. 233-238 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kagayama M.: "Expression of osteocalcin in cementoblasts forming acellular cementum." Journal of Periodontal Research. 32. 273-278 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kagayama M.: "Localization of glycosaminoglycans in periodontal ligament during physiological and experimental tooth movement." Journal of periodontal Research. 31. 229-234 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] I.Mizoguchi: "Localization of type I,II and X collagen and osteocalcin in the three different bone formations." Anatomy and Embryology. 196. 291-297 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] I.Mizoguchi: "Localization of types I,II and III collagen and glycosaminoglycans in the mandibular coxlyle of growing monkeysian immunohistochemical study." Anatomy and Embryology. 195. 127-135 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Saitoh: "Effects of compressive force on the growth and differentiation of midpalatal suture cartilage in rats" 日本矯正歯科学会雑誌. 56. 372-382 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi