| Project/Area Number |
08672376
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Kyushu University |
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Principal Investigator |
INOKUCHI Thoru Research associate, Faculty of Density, Kyushu University, 歯学部, 助手 (00243949)
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| Project Period (FY) |
1996 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Primary Teeth Pulp / Pulptomy / Pulp Cell / PCNA / HgNOR / 生治断髄 / AgNOR / 乳歯 / 歯髄組織 / 被蓋硬組織 |
|---|
| Research Abstract |
We investigated the proliferative and metabolic activities of amputated dental pulp cells of young dogs by means of immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and silver-binding nucleolar organizer region (AgNOR) staining. We examined early stage after pulptomy (from 1 hour to 1 day) so that we investigate early change of the proliferative and metabolic activity of amputated dental pulps. Continuously we examined the 3 days after (the stage that the infiltration of inflammatory cells disappeared) and the 7 days after (the stage of fibroblastic proliferation PCNA-positive pulp cells were detected only in the 3 days and the 7 days, and a significant difference in the positive rate of immunostained cell in the pulpal tissue was detected between each stage. Whereas, the mean number of AgNORs per nucleus increased even in the early stage after pulptomy (the 6 hours post-operatively). Our data suggested that the metabolic activity of dental pulp cells was enhanced early stage after amputation of vital pulp, meanwhile proliferative and differentiative activity of pulp celle occurred only the 3 days and 7 days post-operatively.
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