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Studies on Behavior and Reduction of Unregulated Air Pollutant : Nitroarenes

Research Project

Project/Area Number 08672472
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionKanazawa University

Principal Investigator

HAYAKAWA Kazuichi  Kanazawa University, Faculty of Pharmaceutical Sciences, professor, 薬学部, 教授 (40115267)

Co-Investigator(Kenkyū-buntansha) KAKIMOTO Hitoshi  Kanazawa University Ishikawa Prefectural Institute of Public Health and Environm, 主任技師
ANDO Kyoko  Kanazawa University, Faculty of Pharmaceutical Sciences, Research assistant, 薬学部, 助手 (20228357)
MIKAGE Masayuki  Kanazawa University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (50115193)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsNitroarene / Chemiluminescence / Airborne particulate / Diesel-engine vehicle / Direct-anting mutagenicity / Photo degradation / Formation / ジニトロピレン / ニトロピレン / 発がん性 / 大気汚染物質 / 動態解析
Research Abstract

(1) We introduced another separation column (ODS), a zinc reducer column and a switching valve into the nitroarene analyzer, a HPLC equipped with a chemiluminescence detector, to determine trace levels of nitroarenes.
(2) Benzene-ethanol extracts from diesel exhaust particulates were separated into 5 fractions on a silica-gel column with mixtures of n-hexane, dichloromethane and methanol. The strong direct-acting mutagenicities were observed in the dichloromethane-methanol and methanol fractions in the Ames test using the S.typhimurium YG1024 strain and nitroarenes were mainly in the former fraction of the two. The mutagenic contribution of nitroarenes were 1/3 of the all fractions. The large contribution of nitroarenes to the mutagenicity of urban air suggests that the monitoring of nitroarenes is very important for assessing the risk of air pollutants.
(3) Concentrations of 1,3-, 1,6- and 1,8-dinitropyrenes (DNPs) and 1-nitropyrene (NP) in urban air were in the following decreasing order, Sapporo>Tokyo>Kanazawa. However, the concentrations in downtown areas of the three cities were at similar levels. The relative concentration of DNPs to that of 1-NP was the highest in Sapporo. As a reason, the large ration of diesel-engine vehicles was considered. Their concentrations showed diurnal and seasonal variations.
(4) The relative concentrations of DNPs and 1-NP to polycyclic aromatic hydrocarbons were lower in the suburban areas than in the downtown areas, suggesting that DNPs and 1-NP degradated in the presence of sun light during the transportation. On the other hand, the fact that both 2-nitrofluoranthene (NF) and 2-NP were not detected in diesel exhaust particulates but detected in airborne particulates suggested that these two compounds were formed in air.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] Kazuichi Hayakawa: "An On-line Reduction HPLC/Chemiluminescence Detection System for Nitropolycyclic Aromatic Hydrocarbons and Metabolites" Biomed.Chromatogr.10. 346-350 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Yamamoto: "Novel Approach to the Measurement of Enantiomeric Purity by HPLC Using a Polarized Photometric Detector Equipped with a Split Cell" J.Chromatogr.A. 727. 55-59 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 早川 和一: "受賞研究総説:発癌性ニトロアレーンの超高感度分析法の開発環境研究" 環境研究. 103. 14-20 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 早川 和一: "金沢における未規制大気汚染物質の現状" 金沢大学自然科学研究科地球環境科学専攻紀要. 1. 41-44 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuyoshi Murahashi: "A Sensitive Method for the Determination of 6-Nitrochrysene,2-Nitrofluoranthene and 1-,2- and 4-Nitropyrenes in Airborne Particulates Using HPLCwith CL Detection" Anal.Chim.Acta. 343. 251-257 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Nitroarene Concentrations and Direct-acting Mutagenicities in DieselExhaust Particulates Fractionated by Silica Gel Column Chromatography" Chem.Pharm.Bull.45. 1820-1822 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Yamamoto: "Optimization of a Polarized Photometric Detector Equipped with a Split-type Flow Cell and Its Analytical Application to Oligo-saccharides" J.Biomed.Anal.15. 1383-1387 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Yamamoto: "HPLC/Circular Dichroism Detection Using a Split-Type Flow Cell for Polarized Photometric Detector" Biomed.Chromatogr.11. 362-365 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nariaki Takayama: "Determination of Stimulants ina Single Hair Sample by HPLC Method with Chemiluminescence Detection" Biomed.Chromatogr.11. 25-28 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kyoko Ando: "Theoretical Study of the Elution Profile of a High-Concentration Sample Anion in Non-Suppressed Ion Chromatography" Chromatogr.A. 798. 43-49 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 早川 和一: "創案と開発:ニトロアレーン分析計" ぶんせき. 1997. 323-327 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 東 成見: "漂着重油の多環芳香族炭化水素の組成とその経時変化" 月刊海洋. 29. 593-596 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木津 良一: "重油の毒性評価-変異原性ならびにDNA損傷作用-" 月刊海洋. 29. 597-601 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 早川 和一: "ナホトカ号流出重油及び汚染環境試料中の多環芳香族炭化水素と毒性" 環境化学. 7. 545-552 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 後藤 純雄: "ナホトカ号重油流出事故により海岸に漂着した重油の変異原性及びPAH含量について" 環境化学. 7. 553-559 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 早川 和一: "第2回公開シンポジウム未規制大気汚染物質の挙動と毒性-発がん性関連物質-" 文部省科学研究費補助金基盤研究(C)研究班, 1-34 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 早川 和一: "第3回公開シンポジウム未規制大気汚染物質の挙動と毒性-ダイオキシン,自動車粉じん,流出油-" 文部省科学研究費補助金基盤研究(C)研究班, 1-22 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "An On-line Reduction HPLC/Chemiluminescence Detection System for Nitro-polycyclic Aromatic Hydrocarbons and Metabolites." Biomed.Chromatogr.10. 346-350 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Yamamoto: "Novel Approach to the Measurement of Enantiomeric Purity by HPLC Using a Polarized Photometric Detector Equipped with a Split Cell." J.Chromatogr.A. 727. 55-59 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "State of the Arts : Unregulated Air Pollutants in Kanazawa." Memories of Division of Global Environmental Science and Engineering, Graduate School of Natural Science and Engineering (Doctor's Course), Kanazawa University. 1. 41-44 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Decelopment of Ultra-sensitive Determination Method for Carcinogenic/mutagenic Nitroarenes." Kankyo-Kenkyu. 103. 14-20 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuyoshi Murahashi: "A Sensitive Method for the Determination of 6-Nitrochrysene, 2-Nitrofluoranthene and 1-, 2- and 4-Nitropyrenes in Airborne Particulates Using HPLC with CL Detection." Anal.Chim.Acta.343. 251-257 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Nitroarene Concentrations and Direct-acting Mutagenicities in Diesel Exhaust Particulates Fractionated by Silica Gel Column Chromatography." Chem.Pharm.Bull.45. 1820-1822 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Yamamoto: "Optimization of a Polarized Photometric Detector Equipped with a Split-type Flow Cell and Its Analytical Application to Oligo-saccharides." J.Biomed.Anal.15. 1383-1387 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Yamamoto: "HPLC/Circular Dichroism Detection Using a Split-type Flow Cell for Polarized Photometric Detector." Biomed.Chromatogr.11. 362-365 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nariaki Takayama: "Determination of Stimulants in a Single Hair Sample by HPLC Method with Chemiluminescence Detection." Biomed.Chromatogr.11. 25-28 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kyoko Ando: "Theoretical Study of the Elution Profile of a High-Concentration Sample Anion in Non-Suppressed Ion Chromatography." Chromatogr.A. 798. 43-49 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Nitroarene Analyzer." Bunseki. 1997. 323-327 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masachika Azuma: "Polycyclic Aromatic Hydro-Carbons and their Time Course in Spilled Oil." Kaiyo Manthly. 29. 593-596 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ryoichi Kizu: "Toxicities of Spilled Oil-Mutagenicity and DNA Damage." Kaiyo Manthly. 29. 597-601 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Aromatic Hydro Carbon Concentrations and Toxicities of Oil Spilled from the Nakhodka and Contaminated Environmental Samples." J.Environ.Chem.7. 545-552 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sumio Goto: "Mutagenicity and PAH Contents of the Heavy Oil that Drifted Ashore on the Coast from the Tanker Nakhodka." J.Environ.Chem.7. 553-559 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "An On-line Reduction HPLC/Chemiluminescence Detection System for Nitropolycyclic Aromatic Hydrocarbons and Metabolites" Biomed.Chromatogr.10. 346-350 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Atsushi Yamamoto: "Novel Approach to the Measurement of Enantiomeric Purity by HPLC Using a Polarized Photometric Detector Equipped with a Split Cell" J.Chromatogr.A. 727. 55-59 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 早川和一: "受賞研究総説:発癌性ニトロアレーンの超高感度分析法の開発環境研究" 環境研究. 103. 14-20 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 早川和一: "金沢における未規制大気汚染物質の現状" 金沢大学自然科学研究科地球環境科学専攻紀要. 1. 41-44 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsuyoshi Murahashi: "A Sensitive Method for the Determination of 6-Nitrochrysene,2-Nitrofluoranthene and 1-,2-and 4-Nitropyrenes in Airborne Particulates Using HPLC with CL Detection" Anal.Chim.Acta. 343. 251-257 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kazuichi Hayakawa: "Nitroarene Concentrations and Direct-acting Mutagenicities in Diesel Exhaust Particulates Fractionated by Silica Gel Column Chromatography" Chem.Pharm.Bull.45. 1820-1822 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Atsushi Yamamoto: "Optimization of a Polarized Photometric Detector Equipped with a Split-type Flow Cell and Its Analytical Application to Oligo saccharides" J.Biomed.Anal.15. 1383-1387 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Atsushi Yamamoto: "HPLC/Circular Dichroism Detection Using a Split-Type Flow Cell for Polarized Photometric Detector" Biomed.Chromatogr.11. 362-365 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nariaki Takayama: "Determination of Stimulants in a Single Hair Sample by HPLC Method with Chemiluminescence Detection" Biomed.Chromatogr.11. 25-28 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kyoko Ando: "Theoretical Study of the Elution Profile of a High-Concentration Sample Anion in Non-Suppressed Ion Chromatography" Chromatogr.A. 798. 43-49 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 早川和一: "創案と開発:ニトロアレーン分析計" ぶんせき. 1997. 323-327 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 東 成見: "漂着重油の多環芳香族炭化水素の組成とその経時変化" 月刊海洋. 29. 593-596 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木津良一: "重油の毒性評価-変異原性並びにDNA損傷作用-" 月刊海洋. 29. 597-601 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 早川和一: "ナホトカ号流出重油及び汚染環境試料中の多環芳香族炭化水素と毒性" 環境化学. 7. 545-552 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 後藤純雄: "ナホトカ号重油流出事故により海岸に漂着した重油の変異原性及びPAH含量について" 環境化学. 7. 553-559 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nariaki Takayama: "Determination of simulants in a single Human Hair Sample by High-Performance Liquid Chromatographic Method with Chemiluminescence Detection" Biomed.Chromatogr.11. 25-28 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Noriko Terai: "Estimation of Mutagenicity Caused by Environmental Pollutants Using Chemical Analysis and Ames Test" Jpn.J.Toxicol.Environ.Health. 43. P-10 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuichi Hayakawa: "An Online Reduction HPLC/Chemiluminescence Detection System for Nitropolycyclic Aromatic Hydrocarbons and Metanbolites" Biomed.Chromatogr.10. 346-350 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Atsushi Yamamoto: "Novel Approach to the Measurement of Enantiomeric Purity by High-Performance Liquid Chromatography Using a Polarized Photometric Detector Equipped with a Split Cell" J.Chromatogr.A. 727. 55-59 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 早川和一: "発癌性ニトロアレーンの超高感度分析法の開発" 環境研究. 103. 14-20 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 早川和一: "金沢における未規制大気汚染物質の現状" 金沢大学大学院自然科学研究科 地球環境科学専攻紀要. 1. 41-44 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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