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INTERACTIONS BETWEEN NUCLEIC ACIDS AND METALLOPORPHYRINS

Research Project

Project/Area Number 08672478
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionKUMAMOTO UNIVERSITY (1997-1998)
The University of Tokushima (1996)

Principal Investigator

UNO Tadayuki  Kumamoto University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (00183020)

Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
KeywordsPorphyrin / DNA / RNA / Intercalation / Induced CD Spectroscopy / グルーブ結合 / DNA-RNAハイブリッド / スタッキング / ポリフィリン / RNA2重らせん / グループ結合
Research Abstract

The purpose of this research is to synthesize metalloporphyrins which can recognize a specific nucleotide sequence of nucleic acids and cleave them at a specific position, and to reveal the cleaving activity and interaction mechanism between them. First, I studied the interactions between nucleic acids and a water-soluble cationic porphyrin, tetramethylpyridylporphyrin (TMPyP). I employed synthetic DNA, RNA, and DNA RNA hybrids, and found that (1) TMPyP self-stacks in a minor groove of RNA duplexes, (2) TMPyP intercalates between the base-pairs of hybrid duplexes, and (3) TMPyP either binds to a minor groove electrostatically, or intercalates, depending on the sequence of DNA duplexes. I further studied the way to regulate the binding mode to DNA with the use of triplex formation. It was found that self-stacking and intercalation of the TMPyP were inhibited by the triplex formation, but the groove binding was promoted in that the number of binding sites of the TMPyP increased two-fold. The DNA cleaving activity of iron porphyrin and metal-free porphyrins was studied. The oxidative cleavage by peroxo-compounds was investigated in detail, and addition of imidazoles, which could be axial ligands of the iron, was found to promote the cleaving activity. A porphyrin periphery was introduced to give capability to recognize DNA sequences. In the porphyrin derivative having DNA-intercalating acridirie moiety, CD spectroscopy suggested that the porphyrin located in the minor groove of the DNA.The acridine-free TMPyP self-stacked on the DNA, and the binding mode was distinct that the binding was found to be achieved by the acridine intercalator.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Tadauki Uno: "Binding of meso-Tetrakis(N-methylpyridinium-4-yl)porphyrin to Double Helical RNA and DNA・RNA Hybrids" Inorg.Chem.36. 1676-1683 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayyuki Uno: "Depurination of Adenosine Monophosphates in the Iron Porphyrin-Cumene Hydroperoxide System" J.Inorg.Biochem.67. 99-99 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayuki Uno: "Interactions of Water-soluble Porphyrin with DNA and RNA Triplexes" J.Inorg.Biochem.67. 116-116 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayuki Uno: "Depurination of Adenosine and Deoxyadenosine Monophosphates in the Hemin-Hydroperoxide System" Chem.Pharm.Bull.45. 2110-2112 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tatsushi Mogi: "Substitutions of Conserved Aromatic Amino Acid Residues in Subunit I Perturb the Metal Centers of the Escherichia coli bo-type Ubiquinol Oxidase" Biochemistry. 37. 1632-1639 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayuki Uno: "Biopolymers(Overview)" Polymeric Materials Encyclopedia. 1. 644-652 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宇野,公之: "21世紀の創薬科学" 共立出版, 164 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayuki Uno, Kanya Hamasaki, Masahiko Tanigawa, and Saburo Shimabayashi: "Binding of meso-Tetrakis (N-methylpyridinium-4-yl) porphyrin to Double Helical RNA and DNA・RNA Hybrids" Inorg.Chem.36. 1676-1683 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayuki Uno, Michiyo Shikishima, Mika Shoji, Mayumi Tsuji, and Saburo Shimabayashi: "Depurination of Adenosine Monophosphates in the Iron Porphyrin-Cumene Hydroperoxide System" J.Inorg.Biochem.67. 99 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayuki Uno, Masashi Tanigawa, and Saburo Shimabayashi: "Interactions of Water-soluble Porphyrin with DNA and RNA Triplexes" J.Inorg.Biochem.67. 116 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayuki Uno, Mayumi Tsuji.Mika Shoji, Michiyo Shikishima, Saburo Shimabayashi, Toshiharu Tanaka, and Mitsuru Irikura: "Depurination of Adenosine and Deoxyadenosine Monophosphates in the Hemin-Hydroperoxide System" Chem.Pharm.Bull.45. 2110-2112 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tatsushi Mogi, Jun Minagawa, Tomoyasu Hirano, Mariko Sato-Watanabe, Motonari Tsubaki, Tadayuki Uno, Hiroshi Hori, Haruki Nakamura, Yoshifumi Nishimura, and Yasuhiro Anraku: "Substitutions of Conserved Aromatic Amino Acid Residues in Subunit I Perturb the Metal Centers of the Escherichia coli bo-type Ubiquinol Oxidase" Biochemistry. 37. 1632-1639 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadayuki Uno, and Saburo Shimabayashi: Polymeric Materials Encyclopedia (Salamone, J.C., ed.) "Biopolymers (Overview)". CRC Press, New York, 644-652 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tatsushi Mogi: "Substitutions of Conserved Aromatic Amino Acid Residues in Suburit I Pa-turb the Metal Centers of the Escherichia coli bu-type Ubiquiuol Oxidase" Biochemistry. 37. 15632-1639 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 宇野 公之: "21世紀の創薬科学" 構造生物学と創薬科学, 162 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tadayuki Uno: "Binding of meso-Tetrakis(N-methylpyridinium-4-yl)porphyrin to Double Helical RNA and DNA・RNA Hybrids" Inorganic Chemistry. 36. 1676-1683 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tadayuki Uno: "Depurination of Adenosine Monophosphates in the Iron Porphyrin-Cumene Hydroperoxide System" Journal of Inorganic Biochemistry. 67. 99-99 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tadayuki Uno: "Interactions of Water-soluble Porphyrin with DNA and RNA Triplexes" Journal of Inorganic Biochemistry. 67. 116-116 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tadayuki Uno: "Depurination of Adenosine and Deoxyadenosine Monophosphates in the Hemin-Hydroperoxide System" Chemical and Pharmaceutical Bulletin. 45. 2110-2112 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tadayuki Uno: "The Binding of meso-Tetrakis(N-methylpyridinium-4-yl)porphyrin to Double Helical RNA and DNA・RNA Hybrids" Inorganic Chemistry. 36(印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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