| Project/Area Number |
08672487
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
YOSHIMURA Yoshihiro Hoshi University Faculty of Pharmaceutical Sciences, Associate professor, 薬学部, 講師 (00147894)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Takaho Hoshi University Faculty of Pharmaceutical Sciences, Asistant, 薬学部, 助手 (80210912)
NAKAZAWA Hiroyuki Hoshi University Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (50150173)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | free radical / separation analysys / carboxylic acid / bile acid / micro analysis / electron spin resonance / electrochemical detector / 活性酸素 / 疾患 |
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| Research Abstract |
The roles of free radicals in the body have been investigated in the relationship with various diseases and the detection methods are attracting much attention from various medical fields. Many kind of free radicals in various chemical and biological systems have been successfully detected and identified by an electron spin resonance (ESR) spectroscopy. Carboxylic acids are important as nutritional substrates and metabolites in living organisms. It was found that phenacyl esters, which are derived from phenacyl bromide and carboxyl acids, give rise to the free radicals under the alkaline condition, The p-nitrophenacyl esters derived from p-nitrophenacyl bromide and carboxylic acids were determined by a high-performance liquid chromatography with ESR and ECD as detection. The p-nitrophenacyl bromide was used as a pre-column reagent to derivative the carboxylic acids. The p-nitrophenacyl esters of carboxylic acid gave free radicals by hydrolysis with an alkaline and then the free radicals were detected with ESR and ECD.The ECD peak height was related to the concentration with high linearity in the range of 10-500 pmol for acetic acid, 50-500 pmol for propionic acid, 50-1000 pmol for butyric acid, 0.1-1nmol for valeric and 0.1- 1nmol for hexanoic acid.
The radical chromatography using ESR for HPLC detector was carried out for simultaneous detection of free radicals derived from similar spin adducts of g value.
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