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Gene Expression of Regulatory Protein Regucalcin of Ca^<2+>-Signaling and Its Regulatory Mechanism.

Research Project

Project/Area Number 08672522
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionUniversity of Shizuoka

Principal Investigator

YAMAGUCHI Masayoshi  University of Shizuoka, Graduate School of Nutritional Sciences, Professor., 大学院・生活健康科学研究科, 教授 (70046308)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsRegucalcin / Regulation of gene expression / Liver nuclear / Calcium signaling / Transcription regulation / レギュカルチン遺伝子 / カルシウム / 遺伝子発現
Research Abstract

This investigation was undertaken to clarify the gene expression and its regulatory mechanism of regucalcin which can regulate Ca^<2+> signalin in liver cells due to hormonal stimulation. The following results were obtained.
1.Regucalcin could inhibit RNA synthesis in the nuclei of normal rat liver and regenerating rat liver.
2.Regucalcin mRNA was expressed in the transformed HepG2 cells and its expression was stimulated by insulin.
3.The cDNA clone encoding a regucalcin was isolated from a mouse liver cDNA library and sequenced. Regucalcin mRNA in mice was uniquely expressed in the liver, and that its expression was stimulated by calcium administration.
4.The existence of nuclear factors which bind to the 5'-flanking region of regucalcin gene in rats was found. These identified components was involved in the tissue-specific regulation of regucalcin gene expression.
5.When nuclear proteins obtained from various rat tissues were used in gel mobility-shift assays, tissue-specific formation of a protein-DNA complex was found in the liver and kidney. Ca^<2+>-induced binding of the AP-1 factor to the regucalcin gene was completely inhibited by simultaneous administration of an antagonist of calmodulin. The 5'-flanking region of the rat regucalcin gene ligated to a luciferase reporter gene possessed the promotor activity in H4-II-E hepatoma cells.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Yamaguchi, Masayoshi: "In hibitory effect of calcium-binding protein regacalcin on riborucleic acid synthesis in isolated rat liver nuclei" Malecular and Cellular Biochemistry. 173. 169-175 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murata, Tomiyasu: "Molecular cloning of the cDNA coding for regucalcin and its mRNA expression in mouse liver : The expression is stimulated by calcium" Molecular and Cellular Biochemistry. 173. 127-133 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murata, Tomiyasu: "Expression of calcium-binding protein regucalcin mRNA in the clened human hepatoma cells (HepG2) : Stimulation by insulin." Molecular and Cellular Biochemistry. 175. 163-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murata, Tomiyasu: "Tissue-specific binding of nuclear facfors to the S-Flanking regior of rat gene for calcium-binding protein regucalcin." Molecular and Cellular Biochemistry. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murata, Tomiyasu: "Ca^<2+> administration stimulates the binding of AP-1 factor to the S'-flanking region of the rat gene for the Ca^<2+>-binding protein regular." Biochemical Journal. 329. 157-163 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masayoshi Yamaguchi, Sayuri Ueoka: "Inhibitory effect of calcium-binding protein regucalcin on ribonucleic acid synthesis in isolated rat liver nuclei." Molecular and Cellular Biochemistry. 173. 169-175 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomiyasu Murata, Masayoshi Yamaguchi: "Molecular cloning of the cDNA coding for regucalcin and its mRNA expression in mouse liver : The expression is stimulated by calcium." Molecular and Cellular Biochemistry. 173. 127-133 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomiyasu Murata, Nobuko Synya, Masayoshi Yamaguchi: "Expression of calcium-binding protein regucalcin mRNA in the cloned human hepatoma cells (HepG2) : Stimulation by insulin." Molecular and Cellular Biochemistry. 175. 163-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomiyasu Murata, Masayoshi Yamaguchi: "Tissue-specific binding of nuclear factors to the 5'-flanking region of rat gene for calcium-binding protein regucalcin." Molecular and Cellular Biochemistry. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomiyasu Murata, Masayoshi Yamaguchi: "Ca^<2+> administration stimulates the binding of AP-1 factor to the 5'-flanking region of the rat gene for the Ca^<2+>-binding protein regucalcin." Biochemical Journal. 329. 157-163 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murata,Tomiyasu: "Tissue-specific binding of nuclen factors to the S^1-flanking region of rat gene for calcium-binding protein regucalcin." Molecular and Cellular Biochemistry. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Murata,Tomiyasu: "Ca^<2+> administration stimulates the binding of AP-1 factor to the S^1-flanking region of the rat gene for the Ca^<2+>-binding protein regucalor" Biochemical Journal. 329(1). 157-163 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ueoka, Sayuri: "Inhibitory effect of calcinm-binding protein regucalcin on ribonucleic acid synthesis in isolated rat liver nuclei" Molecular and Cellular Biochemistry. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Murata, Tomiyasu: "Molecular cloning of the cDNA coding for reigucalcir and its mRNA expression in mouse liver : the expression is stimulated by colium" Molecular and Cellular Biochemistry. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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