• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on roles of cofilin (actin/PIP2-binding protein) in activation of phagocytes

Research Project

Project/Area Number 08672551
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionNational Institute of Health Sciences (NIHS)

Principal Investigator

SUZUKI Kazuhiro  NIHS,Div.of Xenobiotic Metabolism and Disposition, Section Chief, 代謝生化学部, 室長 (10154527)

Co-Investigator(Kenkyū-buntansha) ADACHI Reiko  NIHS,Div.of Xenobiotic Metabolism and Disposition, Researcher, 代謝生化学部, 研究員 (10291113)
KASAHARA Tadashi  Kyoritsu College of Pharmacy, Dept.of Biochemistry, Professor (60049096)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsCofilin / Phagocytes / Opsonized zymosan / Okadaic acid / Superoxide / Cytoskeleton / Intracellular pH / Translocation / 細胞内pH変化 / F-アクチン / オカダ酸
Research Abstract

Cofilin, an actin/PLP2-binding phosphoprotein, plays important roles in various cells and tisssues through cytoskeletal reorganization. We studied the roles of cofiln in phagocyte functions using neutrophil-like HL-60 cells and macrophage-like U937 cells. Our major results are as follows :
(1)Opsonized zymosan (OZ) induced rapid dephosphorylation of cofilin and its translocation to plasma membranes on which superoxide was generated and/or OZ particles were engulfed.
(2)Okadaic acid (OA) which is a potent inhibitor for phosphoprotein phosphatase type 2A and type 1, did not inhibit the stimulus-dependent dephosphorylation and translocation of cofilin at 1muM, an enhancing concentration for OZ-induced superoxide production,
(3)To the contrary, OA 5muM, an inhibitory concentration for the OZ-induced respiratory burst, inhibited completely the stimulus-dependent dephosphorylation and translocation of cofilin.
(4)OZ induced decrease in intracellular pH in differentiated U937 cells from 7.3 to 6.8. In the phagocytes, the changes in pH may be an important factor that regulate the F-actin-depolymerizing activity of cofilin.
These results suggest that the stimuli-dependent dephosphorylation and translocation of cofillin are crucial for the activation of phagocytes.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Nagaishi, K., Adachi, R., Kawanishi, T., Yamaguchi, T., Kasahara, T.,Hayakawa, T., Suzuki, K.: "Participation of Cofilin in Opsonized Zymosan-triggered Activation of Neutrophil-like HL-60 Cells through Rapid Dephosphorylation and Translocation to Plasma Membranes." J.Biochem.125(印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagaishi, K., Adachi, R., Matsui, S., Yamaguchi, T., Kasahara, T.,Hayakawa, T., and Suzuki, K.: "Herbimycin A Inhibits Both Dephosphorylation and Translocation of Cofilin Induced by Opsonized Zymosan in Macrophage-like U937 Cells." J.Cell.Physiol.印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi, T., Oshizawa, T., Yamaguchi, T., Suzuki, K., Yamamoto, Y., Hayakawa,T.: "Granulocyte colony-stimulating factor-induced dephosphorylation of a 45 kD cytosolic protein in HL-60 cells differentiating into neutrophils." Br.J.Heamatol.102. 798-803 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 鈴木和博: "白血球の活性化におけるコフィリン(アクチン・PIP2結合タンパク質)の脱リン酸化と細胞膜への移行" 生化学. 69. 254-259 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki, K., Yamaguchi, T., Tanaka, T., Kawanishi, T., Nishimaki-Mogami, T., Yamamoto, K., Tsuji, T.,他: "Activation Induces Dephosphorylation of Cofilin and Its Translocation to Plasma Membranes in Neutrophil-like Differentiated HL-60 Cells." J.Biol.Chem.270. 19551-19556 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki, K., Yamaguchi, T., Oshizawa, T., Yamamoto, Y., Nishimaki-Mogami, T., Hayakawa, T., and Takahashi, A.: "Okadaic acid induces both augmentation and inhibition of opsonized zymosan-stimulated superoxide production by differentiated HL-60 cells." Biochim.Biophys.Acta. 1266. 261-267 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagaishi, K., Adachi, R., Kawanishi, T., Yamaguchi, T., Kasahara, T., Hayakawa, T., and Suzuki, K.: "Participation of Cofilin in Opsonized Zymosan-triggered Activation of Neutrophil-like HL-60 Cells through Rapid Dephosphorylation and Translocation to Plasma Membranes." J.Biochem.in press. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagaishi, K., Adachi, R., Matsui, S., Yamaguchi, T., Kasahara, T., Hayakawa, T., and Suzuki K.: "Herbimycin A Inhibits Both Dephosphorylation and Translocation of Cofilin Induced by Opsonized Zymosan in Macrophage-like U937 Cells" J.Cell. Physiol. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaguchi, T., Oshizawa, T., Yamaguchi, T., Suzuki, K., Yamamoto, Y., and Hayakawa, T.: "Granulocyte colony-stimulating factor-induced dephosphorylation of a 45 kD cytosolic protein in HL-60 cells differentiating into neutrophils." Br.J.Heamatol. 102. 798-803 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki, K.: "Dephosophorylation of cofilin (actin/PIP2-binding protein) and its translocation to plasma membranes in activated leukocytes." (Review in Japanese) Seikagaku. 69. 254-259 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki, K., Yamaguchi, T., Tanaka, T., Kawanishi, T., Nishimaki-Mogami, T., Yamamoto, K., Tsuji, T., Irimura, T., Hayakawa, T., and Takahashi, A.: "Activation Induces Dephosphorylation of Cofilin and Its Translocation to Plasma Membranes in Neutrophil-like Differentiated HL-60 Cells." J.Biol.Chem.270. 19551-19556

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki, K., Yamaguchi, T., Oshizawa, T., Yamamoto, Y., Nishimaki-Mogami, T., Hayakawa, T., and Takahashi, A.: "Okadaic acid induces both augmentation and inhibition of opsonized zymosan-stimulated superoxide production by differentiated HL-60 cells. Possible involvement of dephosphorylation of a cytosolic 21K protein in respiratory burst. Biochim" Biophys. Acta. 1266. 261-267 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 鈴木和博: "白血球の活性化におけるコフィリン(アクチン・PIP2結合蛋白)の脱リン酸化と細胞膜への移行" 生化学. 69・4. 254-259 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鈴木和博: "白血球の活性化におけるコフィリン(アクチン・PIP2結合蛋白)の脱リン酸化と細胞膜への移行" 生化学. 69・4(印刷中). (1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi