• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A research to the participation of endotheline-nitric oxide system in pathophysiology of ischemic disorder of a small intestine and a development for its therapeutic usage.

Research Project

Project/Area Number 08672616
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用薬理学・医療系薬学
Research InstitutionNAGASAKI UNIVERSITY

Principal Investigator

YAMASHITA Kimihiro  NAGASAKI UNIVERSITY FACULTY OF ENVIRONMENTAL STUDIES ASSOCIATE PROFESSOR, 環境科学部, 助教授 (50192399)

Co-Investigator(Kenkyū-buntansha) TANIYAMA Kohtaro  NAGASAKI UNIVERSITY SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (70030898)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsnitric oxide / NOS inhibitors / rat small intestine / ischemia-reperfusion / lactate dehydrogenase / endothelin receptors / receptor autoradiography / ischemia-reperfusion / ileal pouch / NG-nitro-L-arginine methyl ester / nitric oxide / lactate dehydrogenase / rat
Research Abstract

We investigated the role of nitric oxide in the mucosal injury induced by ischemia-reperfusion in the rat small intestine and evaluated the effect of N^G-nitro-L-arginine methyl pester, a potent inhibitor of nitric oxide synthase to the ischemia-related injury of the small intestine. A transient intestinal ischemia was produced in the catheterized ileal segments of rats by occluding the anterior mesenteric artery for 60 min. Nitric oxide metabolites (NO_2^- and NO_3^-) and lactate dehydrogenase activity in perfusates of the intestinal lumen were measured over 5 h periods after reperfusion. The time-course of histological changes in small intestine was also observed. After ischemia-reperfusion, nitric oxide release in the intestinal lumen increased significantly and the dynamics of nitric oxide release correlated with that of lactate dehydrogenase leakage. The administration of N^G-nitro-L-arginine methyl ester (1.0 - 2.5 mg/kg) inhibited this increase of the nitric oxide release and the lactate dehydrogenase leakage, and afforded protection against the mucosal injury induced by ischemia-reperfusion. These results suggest that the nitric oxide production which was accelerated by ischemia-reperfusion of small intestine may possibly participate in thc breakdown of intestinal mucosa after ischemia-reperfusion insult. Next, we investigated endothelin receptors in the rat small intestine following ischemia-reperfusion, using a quantitative receptor autoradiographic method. In correspondence to the part of mucosal injury in the small intestine following ischemia-reperfusion ^<125>I-cndothelin-1 binding sites were increased in number. These binding sites were characteristically the endothelin ETB receptor. Thus, endothelin system may also participate in the breakdown of intestinal mucosa after ischemia-reperfusion insult. This part of the research is now go on more precisely.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kohei Takada: "Participation of nitric oxide in the mucosal injury of intestine induced by ischemia-reperfusion in rats" The Japanese Journal of Pharmacology. 73:SupI. 223 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Sakurai-Yamashita: "Expression of endothelin receptors and nitric oxide synthase in the brain of stroke-prone spontaneously hypertensive rats with cerebral apoplexy" Brain Research. 756:1,2. 61-67 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Sakurai-Yamashita: "Endothelin receptors in kainic acid-induced neural lesions of rat brain" Neuroscience. 81:2. 565-577 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko-Sakurai Yamashita: "Rat peritoneal macrophages express endothelin ET_B but not endothelin ET_A receptors" European Journal of Pharmacology. 338:2. 199-203 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 山下 樹三裕: "虚血性ニューロン死に関するグリアのエンドセリン-NO系" 日本薬理学雑誌. 111:1. 29-36 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kohei Takada: "Participation of nitric oxide in the mucosal injury of intestine induced by ischemia-reperfusion in rats" Jpn J Pharmacol. 73 : Sup I. 223 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Sakurai-Yamashita: "Expression of endothelin receptors and nitric oxide synthase in the brain of stroke-prone spontaneously hypertensive rats with cerebral apoplexy." Brain Res. 756 : 1,2. 61-67 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Sakurai-Yamashita: "Endothelin receptors in kainic acid-induced neural lesions of rat brain." Neuroscience. 81 : 2. 565-577 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuko Sakurai-Yamashita: "Rat peritoneal macrophages express endothelin ETB but not endothelin ETA receptors." Eur J Pharmacol. 338 : 2. 199-203 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kimihiro Yamashita: "The grial endothelin-nitric oxide system in ischemia-related neuronal cell death." Folia Pharmacol Jpn. 111 : 1. 29-36 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kohei Takada: "Participation of mitric oxide in the mucosal injury of intestine induced by ischemia-raperfusion on rats" The Japanese Journal of Pharmacology. 73:SupI. 223P (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuko Sakurai-Yamashita: "Expression of endothelin receptors and nitric oxide synthase in the brain of stroke-prone spontaneously hypertensive rats with cerebral apoplexy" Brain Research. 756:1,2. 61-67 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y,Sakurai-Yamashita: "Endothelin receptors in kainic acid-induced neural lesions of rat brain" Neuroscience. 81:2. 565-577 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuko Sakurai-Yamashita: "Rat peritoneal macrophages express endothelin ET_B but not endothelin ET_A receptors" European Journal of Pharmacology. 338:2. 199-203 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi