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Diagnosis of malaria by RT-PCR

Research Project

Project/Area Number 08672633
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionThe University of Tokyo

Principal Investigator

FURUTA Takahisa  The University of Tokyo, Institute of Medical Science, Research associate, 医科学研究所, 教務職員 (30143514)

Co-Investigator(Kenkyū-buntansha) KIMURA Mikio  The University of Tokyo, Institute of Medical Science, Research associate, 医科学研究所, 助手 (90114462)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsmalaria / RT-PCR / Diagnosis
Research Abstract

We describe here a reverse transcriptase-polymerase chain method for the detection of malaria parasites. Ten in vitro-cultured isolates of Plasmodium falciparum and 16 specimens from patients infected with P.falciparum were used to examine the specificity and sensitivity of the test. The sensitivity of the test was 0.3 parasites per microliter of blood. Specificity was determined by matching the sequences of the specimens' DNA to published sequences of 18S ribosomal RNA gene in the species-specific region. the test proved to be very sensitive and specific for the detection of P.falciparum infection.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Normaznaha Yahaya: "Diagnosis of malaria byenzymetic detection of polymerasechain reaction p-roduct" Southease Asian J.Trop,Med.Publ. Hlth.28,2. 432-433 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木村 幹男: "旅行医学における感染症" 治療学. 31,1. 72-81 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 楽得 康之: "耐性熱帯熱マラリアの治療の進めかた" 感染症の診療,治療. 14,10. 1663-1667 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木村 幹男: "急激な原虫数の増加と黒水熱をきたした重症熱帯熱マラリアの症例" 治療学. 31,1. 105-107 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木村 幹男: "マラリア予防薬としてのメクロキンの副作用の問題" 日本医事新報. 3828. 37-41 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 海老沢 功: "国際旅行医学会に参加して" 日本医事新報. 3820. 44-47 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 藤井 達也: "卵型マラリアVariantの3症例の臨床像" Clinical Parasitology. 8,1. 46-49 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 木村 幹男: "肝腎機能障害,高度の貧血をきたした熱帯熱マラリアの1例" 内科. 81,3. 549-551 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Khoo, Alan: "Nested polymerase chain reaction for the detection of Plasmodium falciparum infection in Malaysia" Trans.Royal Soci.Trop.Med.Hyg. 90. 40-41 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Abdullah, N.R: "Development of a new diagnostic method for Plasmodium falciparum infection using reverse transcriptasepolymerasechain reaction" Amer.J., Trop., Med., Hyg. 54. 162-163 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Lim Kim Chooi, P: "Cytochrome c Oxidase III (COIII) gene of Plasmodium vivax : Complete coding sequence and its expression in the erythrocytic stages of the parasite" Jpn.J.Parasitol. 45. 373-383 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyake, H: "Diagnosis of malaria at convalescent stage posttreatment" J.Travel med. 3. 119-121 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kimura, M: "Caseas of ovale malaria and malariae malaria successfully treated with mefloquine" Jpn.J.Trop.Med.Hyg. 24. 171-175 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Normaznah, Y: "Diagnosis of malaria by enzymatic detedtion of polymerase chain reaction products" Southeast Asian J.Trop.Med.Publ.Hlth. 28. 432-433 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Normaznaha Yahaya: "Diagnosis of malaria by enzymatic detection of polymerase chain reaction products" Southeast Asian J.Trop.Med.Publ.Hlth. 28,2. 432-433 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木村幹男: "旅行医学における感染症" 治療学. 31,1. 72-81 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 楽得康之: "耐性熱帯熱マラリアの治療の進めかた" 感染症の診療・治療. 14,10. 1663-1667 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木村幹男: "急激な原虫数の増加と黒水熱を来した重症熱帯熱マラリアの症例" 治療学. 31,1. 105-107 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木村幹男: "マラリア予防薬としてのXフロキンの副作用の問題" 日本医事新報. 3828. 37-41 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 海老沢功: "国際旅行医学会に参加して" 日本医事新報. 3820. 44-47 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 藤井達也: "卵型マラリアvariantの3症例の臨床像" Clinical Parasitology. 8,1. 46-47 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 木村幹男: "肝腎機能障害、高度の貧血をきたした熱帯熱マラリアの1例" 内科. 81,3. 549-551 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Noor Rain Abdullah: "Development of a new-diagnostic method for Dlasmodium falcjparum in fection using a beverse transcriptase-polymrase chin reaction" Am.J.Trop.Med.Hyg.54,2. 162-163 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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