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Evaluation of platelet activation in vivo by quantitative measurement of myosin phosphorylation.

Research Project

Project/Area Number 08672636
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionYamanashi Medical University, Faculty of Medicine

Principal Investigator

OZAKI Yukio (1997)  Yamanashi Medical University, Faculty of Medicine, Professor, 医学部, 教授 (30134539)

久米 章司 (1996)  山梨医科大学, 医学部, 教授 (50010492)

Co-Investigator(Kenkyū-buntansha) SATOH Kaneo  Yamanashi Medical University, Faculty of Medicine, Assistant, 医学部, 教務職員 (20242662)
YATOMI Yutaka  Yamanashi Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (60200523)
尾崎 由基男  山梨医科大学, 医学部, 助教授 (30134539)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsmyosin phosphorylation / platelet aggregation / light scattering / diabetes mellitus
Research Abstract

We recently developed a new platelet aggregometer, based on light scattering, which can sensitively measure platelet aggregates of small size. Using this instrument, we were able to detect that spontaneous aggregation occurs in patients with diabetes mellitus. Since platelet activation is associated with myosin activation, we then attempted to measure the level of myosin phosphorylation in platelets obtained from diabetes mellitus patients. For this end, we developed a quantitative measurement of myosin phosphorylation, by using an monoclonal antibody which recognizes only the phosphorylated form of myosin, but not ordinary myosin. Previous methods of measuring myosin phosphorylation employed 32P labeling, which often induced artificial activation of platelets, and thus evalution of intact platelets was not possible. Based on our new method using anti-phosphorylated myosin antibody, we were able to evaluate the level of myosin phosphorylation of intact platelets obtained from patients with diabetes mellitus. We found that the level of myosin phosphorylation is significantly increased in patients with diabetes mellitus, and that the level of spontaneous aggregation is well correlated with that of myosin phosphorylation.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Fukuda K, Ozaki Y, et al.: "Phosphorylation of nyosin light chain in resting platelets from NIDDM patients is enhanced.Correlation with spontaneous aggregation." Diabetes. 46. 488-493 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukuda, K., Ozaki, Y., et al.: "Phosphorylation of myosin light chain in resting platelets from NIDDM patients is enhanced. Correlation with spontaneous aggregation." Diabetes. 46. 488-493 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukuda K,Ozaki Y,et al.: "Phosphorylation of myosin light chain in resting platelets from NIDDM patients is enhanced.Correlation with spontaneous aggregation." Diabetes. 46. 488-493 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fukuda K,Ozaki Y,et al.: "Phosphorylation of myosin light chain in resting platelets from NIDDM patients is enhanced." Diabetes. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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