| Project/Area Number |
08672638
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Hamamatsu University, School of Medicine |
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Principal Investigator |
KANNO Takashi Hamamatsu University, School of Medicine, Department of Laboratory Medicine, Professor, 医学部, 教授 (70051406)
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| Co-Investigator(Kenkyū-buntansha) |
YONEKAWA Osamu Clinical Laboratories, Hamamatsu University Hospital, Assistant, 医学部附属病院, 助手 (90158527)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Low density lipoprotein (LDL) / Malondialdehyde (MDA) / MDA-LDL / Oxidative LDL / スカベンジャー受容体 / CETP欠損症 |
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| Research Abstract |
Numerous studies have indicated that oxidative modification of low-density lipoprotein (LDL) plays a critical role in the pathogenesis of atherosclerosis. The malonaldehyde (MDA) modified LDL is a candidate suspected of causing oxidative modification. Thus, the purpose of this study is to elucidate the existence of MDA-modified LDL in circulation and to determine the amounts of this modified LDL in plasma.
We developed sensitive enzyme immunoassay system to determine MDA-modified LDL using monoclonal antibody. Employing this assay system, we determined the reference intervals of normal population. Moreover, the MDA-modified LDL has high affinity to scavenger receptor than normal LDL one. Furthermore, we found MDA-LDL in the particles of small and dense fraction of LDL which was thought to be most strong atherogenic activity in plasma. These results suggest that the measurement of MDA-LDL is one of the useful candidates to evaluate the progress of atheroscrelosis in blood vessels.
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