| Project/Area Number |
08672645
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | KUMAMOTO UNIVERSITY |
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Principal Investigator |
ISHII Toshinori KUMAMOTO UNIVERSITY,COLLEGE OF MEDICAL SCIENCE,ASSISTANT PROFESSOR, 医療技術短期大学部, 助教授 (30093983)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Lymphocytes / Superoxides / Lupus Erythematosus / Apoptosis / Fas / bcl-2 / Cell Adhesion Molecules / Cytokiones / Flow Cytometry / Monocytes |
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| Research Abstract |
1.All of peripheral blood lympocyte (PBL) subsets showed productivity of active oxygen (AO) as they responded to stimulation with phorbor myristate acetate (PMA) .
2.PBLs of systemic jupus erythematosus (SLE) patients produced more AO than ones of healthy persons.Tha cause of high AO production in SLE is speculated as lymphocytes are stimulated with plasma cytokines, for ex-ample IL-lbeta, TNF-alfa and IFN-gamma, and/or cell adhesion molecules such as CDlla and CD29.
3.Amount of AO was correlated positivery with apoptosis rate and negatively with the number of PBLs.And PBLs of healthy persons stimulated with various stimulants due to raising AO production tended to fall into apoptosis.Therefore depletion of PBLs in SLE is suggested to be caused by apoptosis triggered with AO.
4.Fas antigen (CD95) expression was augmented and correlated positively with AO in all PBL subsets of SLE.And bcl-2 expression was also augmented in SLE.SO I suppose that many PBLs in SEL have apoptosis sensitive property and that induction of real apoptosis depends on quantity of bcl-2.In other worts jow bcl-2 PBLs were removed by apoptosis in vivo, and I measured residual high bcl-2 PBLs.
5.When PBLs were cultured with anti-CD3 antibody and anti-Fas antibody, PBLs in SLE expressed more in Fas antigen and less in bcl-2 antigen and apoptosis than in hsalthy persons.This result suggests that there are apoptosis resistant PBLs in SLE.
6.Measurement of AO in PBLs is not suitable for evaluation of disease activity in SLE because of no correlation of AO with almost all clinical manifestations and laboratory data but could be indicator of lesistance to SLE because of negative correlation of AO with anti-DNA antibody.
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