| Project/Area Number |
08680026
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
家政学
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| Research Institution | The University of Tokushima (1997)
Ehime University (1996) |
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Principal Investigator |
KOGA Tetsuro (1997) Tokushima University School of Medicine, Research Associate, 医学部, 助手 (20093859)
宅見 賢二 (1996) 愛媛大学, 教育学部, 教授 (90035428)
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| Co-Investigator(Kenkyū-buntansha) |
TAKUMI Kenji Ehime University, Faculty of Education part time lecturer, 教育学部, 非常勤講師 (90035428)
古賀 哲郎 徳島大学, 医学部, 助手 (20093859)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | millet cultivars / storage protein / prolamin-20kDa / globulin-60kDa / common antigen / two-dimensional electrophoresis / immunoblot / N-terminal amino acid sequence / イムノブロツド / アワ・グルテリン-65KDa / 5M尿素素-2%SDS-50mMトリス / 塩基性サブユニット / PVDF / N-末アミノ酸配列順序 / Total-Porisome / mRNA |
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| Research Abstract |
Storage proteins from millet cultivars containing foxtail, proso and sawa millets are mainly composed of alcohol- and urea- soluble fractions, which are usally known as prolamin and glutelin, respectively. In this studies we attempeted to develop and bringing-up an improved breed of the millet cultivars in plentifulness as well as in nutritional quality. For the purpose of them we firstly made a start on the purification and characterization of the respective major components of foxtail millet prolamin and glutelin proteins. Consequently we obtained following results :
(1) the three millet cultivars share a homologous polypeptide component with a molecular weight of 20 kDa, which is a common antigen among the 3 cultivars.
(2) Foxtail glutelin 60 kDa, which is exclusively extractable with 5 M urea, is antigenically similar to the 60 kDa from the other cultivars.
(3) The N-terminal amino acid sequence of the millet 60 kDa glutein component (MG60) was nearly identical to that of starch-bound starch synthase (EC 2.4.1.11) of rice, barley, corn and wheat. Westan blot analysis using anti-MG60 antibodies demonstrated that these typical cereals contained a similar component to MG60 in the glutelin fractions.
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