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Genetic analysis of IM 60 common antigen from Millet cultivars (foxtail, proso, and sawa millets)

Research Project

Project/Area Number 08680026
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 家政学
Research InstitutionThe University of Tokushima (1997)
Ehime University (1996)

Principal Investigator

KOGA Tetsuro (1997)  Tokushima University School of Medicine, Research Associate, 医学部, 助手 (20093859)

宅見 賢二 (1996)  愛媛大学, 教育学部, 教授 (90035428)

Co-Investigator(Kenkyū-buntansha) TAKUMI Kenji  Ehime University, Faculty of Education part time lecturer, 教育学部, 非常勤講師 (90035428)
古賀 哲郎  徳島大学, 医学部, 助手 (20093859)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsmillet cultivars / storage protein / prolamin-20kDa / globulin-60kDa / common antigen / two-dimensional electrophoresis / immunoblot / N-terminal amino acid sequence / イムノブロツド / アワ・グルテリン-65KDa / 5M尿素素-2%SDS-50mMトリス / 塩基性サブユニット / PVDF / N-末アミノ酸配列順序 / Total-Porisome / mRNA
Research Abstract

Storage proteins from millet cultivars containing foxtail, proso and sawa millets are mainly composed of alcohol- and urea- soluble fractions, which are usally known as prolamin and glutelin, respectively. In this studies we attempeted to develop and bringing-up an improved breed of the millet cultivars in plentifulness as well as in nutritional quality. For the purpose of them we firstly made a start on the purification and characterization of the respective major components of foxtail millet prolamin and glutelin proteins. Consequently we obtained following results :
(1) the three millet cultivars share a homologous polypeptide component with a molecular weight of 20 kDa, which is a common antigen among the 3 cultivars.
(2) Foxtail glutelin 60 kDa, which is exclusively extractable with 5 M urea, is antigenically similar to the 60 kDa from the other cultivars.
(3) The N-terminal amino acid sequence of the millet 60 kDa glutein component (MG60) was nearly identical to that of starch-bound starch synthase (EC 2.4.1.11) of rice, barley, corn and wheat. Westan blot analysis using anti-MG60 antibodies demonstrated that these typical cereals contained a similar component to MG60 in the glutelin fractions.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(17 results)

All Other

All Publications (17 results)

  • [Publications] Takumi Kenji: "Antigenic cross-reactions among major buckwheat proteins" J.Home Econ. Jpn.46. 739-744 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumi Kenji: "Antigenic homology between buckwheat and wheat prolamins" Mem.Fac.Educ.Ehime Univ.,Nat.Sci.16. 31-35 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takumi Kenji: "Polypeptide compositions and antrgenic homologies anong prolamins from Italian,common and Japanese millet cultivars" J.Sci.Food Agric.72. 141-147 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro Koga: "Inducible resistance to zinc and the effect of this ion on the composition of outer membrane proteins in Vibrio parahaemolyticus" J.Gen.Appl.Microbiol.42. 493-500 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro Koga: "Isolation and partial characterization of polymyxin B-resistant isolates of Vibrio parahaemolyticus" Microbiol.Res.151. 43-48 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro.Koga: "The recovery of heat-stressed Vibrio parahacmolyticus inartificial seawater and artificial scawater supplemented with chitin microcosns" J.Gen.Appl.Microbiol.43. 121-123 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kenji Takumi: "Antigenic cross-reactions among major buck wheat proteins." J.Home Econ.Jpn.46. 739-744 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kenji Takumi: "Antigenic homology between buckwheat and wheat prolamins." Mem.Fac.Educ.Ehime Univ., Nat.Sci.16. 31-35 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kenji Takumi: "Polypeptide compositions and antigenic homologies among prolamins from Italian, common and Japanese millet cultivars." J.Sci.Food Agric.72. 141-147 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro Koga: "Inducible resistance to zinc and the effect of this ion on the composition of outer membrane proteins in Vibrio parahaemolyticus" J.Gen.Appl.Microbiol.42. 493-500 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro Koga: "Isolation and partial characterization of polymyxin B-resistant isolates of Vibrio parahaemolyticus" Microbiol.Res.151. 43-48 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro Koga: "The recovery of heat-stressed Vibrio parahaemolyticus in artificial seawater and artificial seawater supplemented with chitin microcosms" J.Gen.Appl.Microbiol.43. 121-123 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro Koga: "The recovery of Reat-stressed Vibrio parahaemolaticus in artificial seawater and artifictal seawater supplemented with chitin microcosms" J.Gen.Appl.Microbiol.43(2). 121-123 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kenji Takumi et al.: "Polypeptide compositions and antigenic eromologies amoug Prolamins from Halion,Commor and Japanese millet cultivars" J.Sci.Food Agric.72. 141-147 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kenji Takumi et al.: "Immunocloincal Gossroctivity between of blins from Backhest and lndigo sods." Biosci.Biotech.Biocham.59. 1971-1972 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Koga & K.Takumi: "Inducible rosistonce to Zlh c and the effect of Puision on the composition of auter munbeme poleins in Vibrio paraheemilyticus." J.Gen.Appl.Microbiol.42. 493-500 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tetsuro Koga & K.Takumi: "Isslation and portial charocterzation of Polymison Bresistant of V. parohomdyticus" Microbial Reserch. 151. 43-48 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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