| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
Carotenoid (0.0186 mu M) was dissolved in lml of 4 : l benzene-methanol, and this was added to lml of isopropanol containing 0.1888 mu M of photosensitizer dye, methylene blue. Singlet oxygen was generated by illumination with Schott light source at 10cm full power, aliquots taken at intervals and the pigment concentration determined spectrophotometrically, The relative reactivity of each carotenoid was estimated by measuring the percentage destruction of each pigment after 5 minutes. Lutein exhibited the greatest activity of the carotenoids investigated, and then lycopene, beta-carotene, crocetin, violaxanthin and neoxanthin showed anti-oxidative potential, in that order.
Carotenoid was dissolved in lml of chlorobenzene and the solution preincubated at 60ﾟC for 5 minutes. Carbon-centered organic free radicals were thermally generated by the addition of 250 mu I of azo-iso-butyronitrile (AIBN) in chlorobenzene, and the reaction followed spectrophotometrically by measuring the decreasein absorbance at the wavelength maximum of each carotenoid. The relative reactivity of each carotenoid with AIBN was estimated by measuring the percentage pigment destroyed after 5 minutes. Lycopene exhibited the greatest activity of the carotenoids investigated, and then beta -carotene, neoxanthin, crocetin, lutein and violaxanthin showed anti-oxidizability in that order.
From the above results, we can affirm that crocetin from gardenia, lycopene from tomatoes, beta-carotene, lutein, violaxanthin and neoxanthin from green leaves have anti-oxidizabilty.
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