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Estimation of Biiological Purification Potentials of Petroleum Hydrocarbons in Estuarinrin Sediment of Urban River

Research Project

Project/Area Number 08680551
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental dynamic analysis
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

KOBAYASHI Akiko  Tokyo Univ. of Agric & Technol., Dept. of Technol., Assistant of Professor, 工学部, 助手 (80186725)

Co-Investigator(Kenkyū-buntansha) 村上 昭彦  東京農工大学, 工学部, 教授 (20015043)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPetroleum Hydrocarbons / Aerobic Degradation / Anaerobic Degradation / Sediment / Microbial Purification / Maximum Degradation Rate / 存在量 / 環境因子
Research Abstract

Estuarine sediments in urban rivers contain petroleum hydrocarbons which are derived from numerous deverse sources such as motor vehicle exhaust, fuel combustion products, airborne particulate matter, and high-boiling temperature petroleum distilllates. We determine the aerobic and anaerobic biodegradation rates of the substrates of petroleum hydrocarbons using estuarine sediment from the Tama River in urban Tokyo.
Known amounts of the selected petroleum hydrocarbons were added to a sediment sulurry which was prepared by mixing the sediment with river bottom water (sediment : water, 1 : 1. v/v). We added petroleum hydrocarbons to a test tube (100ml volume), and then added 10ml of sediment slurry. We periodically extracted petroleum hydrocarbons from the sediment slurry using ethyl acetate, and then determined their concentration by FID gas chromatography or spectrophotometer.
When the maximum microbial purification potentials of petroleum hydrocarbons are estimated under anaerobic conditions, the degradation ratio by the stationary culture is better than by the shaking culture. For example, the degradation ratio of hexadecane for 30 days is 45% by shaking and 89% by stationary. In hexadecanol, it is 31% by shaking and 97% by stationary for 15 days. In p-chlorphenol, it is 6% by shakin and 94% by stationary for 25 days. In m-aminobenzoic acid, it is trace by shaking and 96% by stationary for 19 days. It is obvious that the reason is not the cell damage by shaking or short contact time between substrate and degrading cell, but adaptaion of cultivation condition. The[ sediment core experiments suggested that aerobic microorganisms in oxidized surficail sediment were more capable of degrading petroleum hydrocarbon than microorganisms in anaerobic sediment. The bio-purification potential of hexadecane, phenanthrene and anthracene in Tama River were evalutated in anaerobic core sediment.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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