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Biodegradation of hazardous organochemicals by bacteria producing cytochrome P450

Research Project

Project/Area Number 08680609
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境保全
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

IKATSU Hisayoshi  OKAYAMA Univ., Administration Center for Environmental Science and Technology, Staff assistant, 環境管理センター, 助手 (10260663)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordscytochrome P450 / hazardous organochemicals / biodegradation / 2-ethoxyphenol / purification of enzyme
Research Abstract

Bioremediation is emerging as one of the most promising technologies for the removal of hazardous organochemicals from the environment. For this, the screening of potential organochemicals degrading bacteria is one of the key step. Cytochrome P450 are widely distributed in the biosphere and catalyze a remarkably broad range of chemical reactions on an equally broad range of substrates. Therefore, the bacteria producing cytochrome P450 are good candidates for biodegradation of hazadous organochemicals.
In this study, we isolated 7 strains of bacteria producing cytochrome P450 from environmental soil and water by using classical enrichment techniques. Six strains of isolated bacteria grew on 2-ethoxyphenol as sole carbon and energy sources. Another one grew on camphor as sole carbon and energy sources. A possibility exists that the bacteria producing cytochrome P450 are widespread in nature and bacterial cytochrome P450 is involved in the biodegradation of hazardous chemicals in nature.
St … More rain EJ that isolated from soil using 2-ethoxyphenol containing medium was investigated. The growth of EJ was observed on 2-ethoxyphenol, 4-ethoxybenzoic acid, 2-methoxyphenol, 4-methoxybenzoic acid and n-hexadecane. The organism could also be cultivated on lactose, glucose, succinic acid, ethanol and glycerol. Of the growth substrates tested, the presence of P450 was detected only in extracts of cell grown on 2-ethoxyphenol or 4-ethoxybenzoic acid.
The kinetics of P450 induction by 2-ethoxyphenol was investigated. The enzyme was induced during the exponential growth phase and reached a maximum level of 0.5 nmol/mg-protein at the end of exponential growth phase. The P450 level then rapidly decreased at stationery phase. The cell free extract of the 2-ethoxyphenol-grown EJ metabolized not only 2-ethoxyphenol but also benzene, xylene, toluene, 1,3-dichloropropene, carbon tetrachloride, 1,1,1-trichloroethane. The P450 can display a remarkable chemical flexibility toward substrate. Thus, the P450 from strain EJ may be useful for the removal of hazardous organochemicals from environment.
The cell free extract from EJ was subjected to gel filtration and anion exchange chromatography. The P450 fraction from the anion exchange column had a specific content of P450 of 17.1 nmol/mg/protein. Molecular weight calibration of gel filtration indicated an approximate molecular weight of 45,000 for the P450 containing peak. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 井勝 久喜, 篠田 純男: "微生物によるセレン(IV)の濃縮と黒煙炉原子吸光法による定量" 環境技術. 25・10. 618-625 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 田中 雅邦・藤元 教尊・井勝 久喜 高木 茂明: "複雑組成無機廃液中のセレン酸除去" 環境制御. 19. 21-25 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] IKATSU, H., SHINODA, S., NAKAJIMA, T.: "CYP2E1 Level of Rat Liver Injured by the Interaction between Carbon Tetracheloride and Chloroform" Journal of Occupational Health. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ikatsu, H.and SHINODA,S.: "Determination of selenium (IV) in water by graphite furnace atomic absorption spectrometry after preconcentration with microorganism. (Japanese with English Abstract)" Environmtnetal Conservation Engineering. 25 (10). 618-625 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] TANAKA,M., FUJIMOTO,M., IKATSU,H.and TAKAGI,S.: "Removal of selenate from laboratory wastewater. (Japanese)" Environment Research and Control. 19. 21-25 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] IKATSU,H., SHINODA,S.and NAKAJIMA,T.: "CYP2E1 level of rat liver injured by the interaction between carbon tetrachloride and chloroform." Journal of Occupational Health. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 田中雅邦・井勝久喜 他: "複雑組成無機廃液中のセレン酸除去" 環境制御. 19. 21-25 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 井勝久喜 他: "微生物によるセレンの濃縮と黒煙炉原子吸光法による定量" 環境技術. 25. 618-625 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.IKATSU et al.: "Pre-concentration of Selenium by Microorganisms and Analysis by Graphite Furnace Atomic Absorption Spectrometry" Proceedings of the Third Asian Symposium on Academic Activity for Waste Treatment and Resources 1996. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.KINO et al.: "Biodegradation of Hazadous Chemicals ; Isolation and Characterization of Bacteria Containing Cytochrome P-450" Proceedings of the Third Asian Symposium on Academic Activity for Waste Treatment and Resources 1996. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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