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ACh recognition and modulation of the inhibitory nicotinic ACh receptor

Research Project

Project/Area Number 08680723
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionKOBE UNIVERSITY

Principal Investigator

NAGAHAMA Tatsumi  FACULTY OF SCIENCE,KOBE UNIVERSITY,ASSOCIATE PEOFESSOR, 理学部, 助教授 (70145001)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsinhibitory ACh receptor / Cl^- channel / Lophotoxin / Bippinatin / nicotinic receptor / ACh binding site / Aplysia / IJP / Cl-チャンネル / シナプス修飾 / Bipinnatin
Research Abstract

Firing of Aplysia cholinergic MA neurons produces IPSPs in the jaw-closing (JC) motor neurons and IJPs in the JC muscle fibers. We previously demonstrated that the ACh receptors producing these inhibitory responses may be nicotinic, and the muscle receptors may be associated with Cl^- channel. Lophotoxin and the three analogues (bippinatin-A,B,C)are known to react covalently with Tyr^<190> in the ACh binding site of the receptor and inhibit the function. Bimolecular reaction constants decreased in the order bippinatin-B>A>C for the ACh binding site. In the present study the effects of these analogues on the IJPs or IPSPs were explored and compared with those on the excitatory responses to know the ACh recognition of the inhibitory ACh receptors. Bippinatin-B largely reduced the size of the IJPs, while bippinatin-A and -C increased it. The voltage clamp experiments of the JC neurons showed that the reversal potential of the synaptic current response was shifted toward more positive when the Cl^- was imjected into the JC neurons, suggesting that the ACh receptor in the JC neurons also may be associated with Cl^- channel. Bippinatin-B largely reduced the size of the IPSPs while bippinatin-A initially increased it (ca.25%) and reduced it later. The effect of bippinatin-C was more complex. Initially bippinatin-C largely increased the IPSP size (ca.200%) and then reduced it. After that the MA firing tended to produce the large depolarizing response and finally all responses ceased. These results suggest that the ACh binding site of the inhibitory receptor may be similar to that of the excitatory ACh receptor but the fine molecular structure around the binding site may be different between them.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] T.Nagahama: "Patterned jaw movements and the motor neuron activity during rejection of seaweed in Aplysia kurodai." J.Comp.Physiol.A. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nagahama: "Synaptic modulation appeared during rejection of seaweed in Aplysia kurodai." Soc.Neurosci.Abstr.23. 1046 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤 悦朗(50音順): "軟体動物腹足類における感覚情報処理と連合学習機構" 生物物理. 37. 150-154 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nagahama: "Patterned jaw movements during rejection of seaweeds in Aplysia kurodai." Soc.Neurosci.Abstr.22. 1405 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Inoue: "Cl channels as a cholinergic ACh receptor responsible for generation of inhibitory junction potential in Aplysia buccal muscle cells." Jap.J.Physiol.44. S149-155 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nagahama: "Acetylcholine-activated chloride channels producing an inhibitory junction potential in buccal muscle cells of Aplysia." Proc.R.Soc.Lond.B. 254. 275-280 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagahama, T.& Shin, N.: "Patterned jaw movements and the motor neuron activity during rejection of seaweed in Aplysia kuroadai." J.Comp.Physiol.A. (in press.). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagahama, T.: "Synaptic modulation appeared during rejection of seaweed in Aplysia kuroadai." Soc.Neurosci.Abstr.23. 1046 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito.E., Kawahara, S., Sakakibara, M & Nagahama, T.: "Mechanism of sensory processing and learning in the gastropods." Biophysics. 37. 150-154 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagahama, T.& Shin, N.: "Patterned jaw movements during rejection of seaweeds in Aplysia kurodai." Soc.Neurosci.Abstr.22. 1405 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagahama, T.& Shin, N.: "Rejection of natural seaweeds in Aplysia kurodai." Jap.J.Physiol.46 (S). A178 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagahama, T.: "Neural mechanism switching patterned movements in the feeding system of Aplysia kurodai." Jap.J.Physiol.45 (S2). S11 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagahama, T., Inoue, I.& Takata, M.: "Acetylcholine-activated two types of ion channels produce hyperpolarization or depolarization at different blocks of buccal muscle in Aplysia." J.Physiol.480.P.126P (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Inoue, I., Nagahama, T.& Takata, M.: "Cl^- channels as a cholinergic ACh receptor responsible for generation of inhibitory junction potential in Aplysia buccal muscle cells." Jap.J.Physiol.44. S149-155 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagahama, T., Inoue, I.& Takata, M.: "Acetylcholine-activated chloride channels produce an inhibitory junction potential in buccal muscle cells of Aplysia." Proc.R.Soc.Lond.B. 254. 275-280 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nagahama: "Patterned jaw movements and the motor neuron activity during rejection of seaweed in Aplysia kurodai" J.Comp.Physiol.A. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Nagahama: "Synaptic modulation appeared during rejection of seaweed in Aplysia kurodai." Soc.Neurosci.Abstr.23. 1046 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 伊藤悦朗(50音順): "軟体動物腹足類における感覚情報処理と連合学習機構" 生物物理. 37. 150-154 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Nagahama: "Patterned jaw movements during rejection of seaweeds in Aplysia kurodai." Soc.Neurosci.Abstr.22. 1405 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] I.Inoue: "Cl^- channels as a cholinergic ACh receptor responsible for generati of inhibitory junction potential in Aplysia buccal muscle cells." Jap.J.Physiol.44. S149-155 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Nagahama: "Acetylcholine-activated chloride channels produce an inhibitory junction potential in buccal muscle cells of Aplysia" Proc.R.Soc.Lond.B. 254. 275-280 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagahama,T: "Acetylcholine-activated chloride channels produce an inhibitory junction potential in buccal muscle cells of Aplysia." Proc.R.Soc.Lond.B. 254. 275-280 (1993)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue,I.: "Cl channels as a cholinergic ACh receptor responsible for generation of inhibitory junction potential in Aplysia buccal muscle cells." Jap.J.Physiol.44. s149-155 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagahama,T: "Acetylcholine-activated two types of ion channels produce hyperpolarization or depolarization at different blocks of buccal muscle an Aplysia" J.Physiol.Lond. 480P. 126 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagahama,T: "Patterned jaw movements during rejection of seaweeds in Aplysia kurodai." Soc.Neurosci.Abstr.22. 1405 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagahama,T: "Rejection of natural seaweeds in Aplysia kurodai." Jap.J.Physiol.46(s). S178 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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