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Function of Ad4BP in the process of gonadal sex differentiation

Research Project

Project/Area Number 08680745
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research Institution(National Institute for Basic Biology) Okazaki National Research Institutes (1997)
Kyushu University (1996)

Principal Investigator

MOROHASHI Ken-ichirou  National Institute for Basic Biology, Professor, 基礎生物学研究所, 教授 (30183114)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsAd4BP / DAX-1 / transcription / testis / ovary / adrenal cortex / P450 / steroid / 精巣 / 卵巣
Research Abstract

Immunohistochemical analyzes with anti-Ad4BP revealed that the gonads and adrenal cortex, major steroid hormone producing tissues, are originated from an identical cell population. The Ad4BP immunopositive cells divide into two populations which thereafter give rise to primordia for the gonads and adrenal cortex. In the process, one of the interesting issues is why sex differentiation occur in the gonadal but not in the adrenocortical development. In the study we examined the mechanism of gonadal sex differentiation.
The expression and function of transcription factors, Ad4BP and DAX-1, were examined extensively. These transcription factors are expressed in the gonads and adrenal cortex. However, the expression profiles are not identical, ie the positive cells for Ad4BP are not always positive for DAX-1. Such distinct expression between the two transcription factors was seen in the genital ridge of 11.5 dpc mouse fetuses. With respect to the function of DAX-1, DAX-1 was shown to repress the transcription of the steroidogenic P450 genes activated by Ad4BP.The Dax-1 ge transcription was revealed to be under the control of Ad4BP.
It is supposed that DAX-1 functions as the supressor against the transcription of the genes showing gonadal and adrenal specific expression. Our results indicated that the Dax-1 gene is regulated by Ad4BP.Nevertheless, thedistribution was not identical to that of Ad4BP,indicating that unknown transcription factors in addition to Ad4BP are probably needed for the transcription of the Dax-1 gene. Concerning the transcriptional regulation of the P450 genes, negative regulation by DAX-1 should be considered to understand their whole regulation.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] M.Nomura, et al: "Autoregulatory Loop in the Regulation of the Mammalian Ftz-F1 Gene." J.Biol.Chem.271. 8243-8249 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] O.Hatano, et al: "Identical Origin of Adrenal Cortex and Gonads Revealed by Expression of Ad4BP/SF-1." Genes to Cells. 1. 663-671 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Morohashi, et al: "Transcritption of Steroidogenic Cytochrome P450 Genes and for the Establishment of the Reproductive Function." FASEB J.10. 1565-1577 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Leers-Suchete, et al: "Synergistic Activation of the Human Type II 3β-Hydroxysteroid Dehydrogenase/D^5D^4 Isomerase Promoter by the Transcription Factor Steroidogenic Factor-1/Adrenal 4-Binding Protein and Phorbol Ester" J.Biol.Chem.272. 7960-7967 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Lund, et al: "Trnascriptional regulation of the bovine CYP17 gene by cAMP." Steroid. 62. 43-45 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.I.Mason, et al: "The regulation of 3β-hydroxysteroid dehydrogenase expression." Steroid. 62. 164-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Morohashi: "The ontogenesis of the steroidogenic tissues." Genes to Cells. 2. 95-106 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masatoshi Nomura, Hajime Nawata, & Ken-ichirou Morohashi: "Autoregulatory Loop in the Regulation of the Mammalian Ftz-F1 Gene" J.Biol.Chem.271. 8243-8249 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Osamu Hutano, Akira Takakusu, Masatoshi Nomura, & Ken-ichirou Morohashi: "Identical Origin of Adrenal Cortex and Gonads Revealed by Expression of Ad4BP/SF-1" Genes Cells. 1. 663-671 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ken-ichirou Morohashi & Tsuneo Omura: "Ad4BP/SF-1, a Transcription Factor, Essential for the Transcription of Steroidogenic Cytochrome P450 Genes and for the establishment of the reproductive Function" FASEB J. 10. 1569-1577 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Susan Leers-Sucheta, Ken-ichirou Morohashi, J.lan Mason, & Michael H.Melner: "Synergistic Activation of the Human Type II 3beta-Hydroxysteroid Dehydrogenase /D^5D^4 Isomerase Promoter by the Transcription Factor Steroidogenic Factor-1/Adrenal 4-Binding Protein and Phorbol Ester" J.Biol.Chem.272. 7960-7967 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Johan Lund, marit Bakke, Gunnar Mellgren, Ken-ichirou Morohashi, & Stein-Ove Doskeland: "Trnascription regulation of the bovine CYP17 gene by cAMP" Steroid. 62. 43-45 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.lan Mason, Diane Keeney, lan M.Bird, William E.Rainey, Ken-ichirou Morohashi, Susan Leers-Sucheta, & Michael H.Melner: "The regulation of 3beta-hydroxysteroid dehydrogenase expression" Steroid. 62. 164-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ken-ichirou Morohashi: "The ontogenesis of the steroidogenic tissues" Genes Cells. 2. 95-106 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Leers-Sucheta,et al: "Synergistic Activation of the Human Type II 3β-Hydroxysteroid Dehydrogenase/D^5D^4 ISomerase Promoter by the Transcription Factor Steroidogenic Factor-1/Adrenal 4-Binding Protein and Phorbol Ester." J.Biol.Chem.272. 7960-7967 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.Lund,et al: "Trnasciptiona regulation of the bovine CYP17 gene by cAMP." Steroid. 62. 43-45 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.I.Mason,et al: "The regulation of 3β-hydroxysteroid dehydrogenase expression." Steroid. 62. 164-168 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Morohashi: "The ontogenesis of the steroidogenic tissues." Genes to Cells. 2. 95-106 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 諸橋憲一郎, 他: "生殖腺、副腎皮質の形成と転写因子" 実験医学. 15. 78-82 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 諸橋憲一郎: "性ホルモン・性分化とノックアウトマウス" 内分泌・糖尿病科. 4・(2). 137-149 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masatoshi Nomura: "Autoregulatory loop in the regulation of the mammalian FtzF1 gene." J.Biol.Chem.271. 8243-8249 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Osamu Hatano: "Identical origin of adrenal cortex and gonads revealed by expression of Ad4BP/SF-1." Genes to Cells. 1. 663-671 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shoichiro Ikuyama: "Follicle stimulating hormone-β subunit gene is expressed in parallel with a transcription factor, Ad4BP/SF-1 in human pituitary adenomas." Clin.Endocrinol.45. 187-193 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koichi Oba: "Structural characterization of human Ad4BP (SF-1) gene." Biochem.Biophys.Res.Commun.226. 261-267 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ken-ichirou Morohashi: "Ad4BP/SF1,a transcription factor,essential for the transcription of steroidogenic cytochrome P450 genes and for the establishment of the reproductive function" FASEB J.10. 1569-1577 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Susan Leers-Sucheta: "Synergistic activation of the human type II 3β-hydroxysteroid dehydrogenase/Δ^5Δ^4 isomerase promoter by the transcription factor steroidogenic factor-1/adrenal 4-binding protein and phorbol ester." J.Biol.Chem.(in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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