| Project/Area Number |
08680771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
KOKAI Yasuo DEPARTMENT OF PATHOLOGY SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE, 医学部, 講師 (20178239)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Michio DEPARTMENT OF PATHOLOGY SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE, 医学部, 教授 (00045288)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | tight junction / barmotin / oclcudin / zo-1 / cancer / barrier / 分子病理学 / ZO-1 / Barmotin / 生体バリア / 分子モーター / SMC / 生体防御 |
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| Research Abstract |
Tight junctions localize uppermost of apposing epithelium and a class of endothelium. Tight junctions serve as a barrier for paracellular pathway and regulate traffics of ion, small molecules and cells. In the present projects, we studied molecular structure of tight junction proteins and those expression in normal and disordered tissues both in vivo and in vitro. To perform these studies, we cloned rat occludin and produced polyclonal antibodies against barmotin and occludin. As barmotin has been suggested to belong SMC mechanochemical motor protein, we identified four more SMC proteins in human and rats. Study using human colon cancer revealed that abberant expression of tight junction proteins were observed. The pattern of dysregulation of tight junction proteins were also observed in mouse intestinal cell lines upon treatment of bacterial lipopolysaccharide. Taken together, these observations suggested that tight junction proteins play an important role in both normal and disordered tissues. The current study has elicited the role of tight junction proteins in various conditions.
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