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ENU mutagenesis and a trial to isolate mutant alleles for specific genes located in the sex-determining region of the Japanese medaka.

Research Project

Project/Area Number 08680788
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNiigata University

Principal Investigator

SAKAIZUMI Mitsuru  Professor, Department of Environmental Science, Faculty of Science, Niigata University, 理学部, 教授 (40175360)

Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
KeywordsMedaka / sex chromosome / sex-determining gene / mutagenesis / ethylnitrosourea / sex reversal / 動原体マッピング / 組換抑制領域
Research Abstract

Ethylnitrosourea mutagenesis of spermatogonia in male medaka was performed to isolate mutant alleles in the sex-determining region. We used a congenic strain, Hd-rR.Y^<HNI>, the male of which has a genetic background derived from the Hd-rR strain, and has only the chromosomal region including the sex-determining factor (and r locus) from the HNI strain in its Y chromosome.
1. Sex-reversed XY females were mated with XY males, and YY males were obtained. A sex-linked DNA marker SL1 was used for detection of YY males.
2. YY (RR ; red) and XY (Rr ; red) males were mutagenized with 1mM ENU and mated with non-treated XX (rr ; white) females.
3. A total of 746 offspring (402 males and 344 females) were produced from XY males. All females were white and 391 males red. Eleven white males were demonstrated to be sex-reversed XX individuals by mating expriment. All 269 male off spring from the YY males were red.
4. Thus no mutants were obtained in the presentstudy. In order to get mutant alleles in the sex-determining region, it is necessary to continue mutagenesis experiment.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Matsuda, Masaru: "Geogra-phic variation and diversity in the mitochondrial DNA of the medaka, Oryzias latipes, as determined by restriction endonuclease analysis." Zool.Sci.14. 517-526 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, Masaru: "Mitochondrial DNA variation in the Korean wild population of medaka, Oryzias latipes." Korean J.Limnol.30 :. 119-128 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, Masaru: "Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes." Genes Genet.Syst.,. 72 :. 263-268 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, Masaru: "Identification of the sex chromosomes of the medaka, Oryzias latipes, by fluorescence in situ hybridization." Cytogenet.Cell Genet.82. 257-263 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, Masaru: "Male-specific restriction of recombination frequency in the sex chromosomes of the medaka, Oryzias latipes." Genet.Res.(Cambridge),. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, M., Yonekawa, H., Hamaguchi S.Sakaizumi, M.: "Geographic variation and diversity in the mitochondrial DNA of the medaka, Oryzias latipes, as determined by restriction endonuclease analyasis." Zool.Sci.14. 517-526 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, M., Yamagishi, T., Sakaizumi, M.Jeon S-R.: "Mitochondrial DNA variation in the Korean wild population of medaka, Oryzias latipes." Korean J.Limnol.30. 119-128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, M., Kusama, T., Kurihara, Y., Oshiro, T., Hamaguchi, S.Sakaizumi, M.: "Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes." Genes Genet.Syst.72. 263-268 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda M., Matsuda C., Hamaguchi S., Sakaizumi M.: "Identification of the sex chromosomes of the medaka, Oryzias latipes, by fluorescence in situ hybridization." Cytogenet.Cell Genet.82. 257-263 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda M., Sotoyama S., Hamaguchi S., Sakaizumi M.: "Male-specific restriction of recombination frequency in the sex chromosomes of the medaka, Orzias latipes." Genet.Res.(Cambridge). (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda,Masaru: "Identification of the sex chromosomes of the medaka,Oryzias latipes,by fluorescence in situ hybridization." Cytogenet.Cell Genet.82. 257-263 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsuda,Masaru: "Male-specific restriction of recombination frequency in the sex chromosomes of the medaka,Oryzias latipes." Genet.Res.(Cambridge),. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsuda,Masaru: "Geogra-phic variation and diversity in the mitochondrial DNA of the medaka,Oryzias latipes,as determined by restriction endonuclease analysis." Zool.Sci.14. 517-526 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsuda,Masaru: "Mitochondrial DNA variation in the Korean wild population of medaka,Oryzias latipes." Korean J.Limnol.30. 119-128 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsuda,Masaru: "Isolation of a sex chromosome-specific DNA sequence in the medaka,Oryzias latipes." Genes Genet.Syst.72. 263-268 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsuda, Masaru: "Geographic variation and diversity of in the mitochondrial DNA of the medaka, Oryzias latipes, as determined by restriction endonuclease analysis." Zoological Science. 14(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda, Masaru: "Mitochondrial DNA variation in the Korean wild population of medaka,Oryzias latipes." Korean Journal of Limnology. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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