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Regulation of region-specific neural genes in zebrafish early embryos

Research Project

Project/Area Number 08680789
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNagoya University

Principal Investigator

TAKEDA Hiroyuki  Nagoya University, Grad.Sch.Science Associate Professor, 理学研究科, 助教授 (80179647)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsneural induction / mesoderm induction / zebrafish / organizer / FGF / otx2 / pax
Research Abstract

Analyzes using amphibian embryos proposed that induction and anteroposterior patterning of the central nervous system is initiated by signals that are produced by the organizer and organizer-derived axial mesoderm. However, we show here that the initial anteroposterior pattern of the zebrafish central nervous system depends on the differential competence of the epiblast, and is not imposed by organizer derived signals. This anteroposterior information is present throughout the epiblast in ectodermal cells that normally give rise both to neural and non-neural derivatives. Because of this information, organizer tissues transplanted to the ventral side of the embryo induce neural tissue but the anteroposterior identity of the induced neural tissue is dependent upon the position of the induced tissue within the epiblast. Thus, otx2, an anterior neural marker, was only ever induced in anterior regions of the embryo, irrespictive of the position of the grafts. Similarly, hoxa-1, a posterior neural marker was induced only in the posterior regions. Furthermore, the boundary of each ectopic expression domain on the ventral side was always at an equivalent latitude to that of the endogenous expression of the dorsal side of the embryo. The anteroposterior specification of the epiblast is independent of the dorsoventral specification of the embryo because neural tissues induced in the ventralized embryos also showed anteroposterior polarity. Cell transplantation and RNA injection experiments showed that non-axial marginal mesoderm and FGF signalling is required for anteroposterior specification of the epiblast. However, the requirement for FGF signalling is indirect in that cells with compromised ability to respond to FGF can still respond to anteroposterior positional information.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Terumi Miyagawa: "Specification of posterior midbrain region in zebrafish neuroepithelium" GENES TO CELLS. 1. 369-377 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toshiro Mizuno: "Mesoderm induction in zebrafish" Nature. 383. 131-132 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 武田洋幸: "頭部神経は外胚葉の前方でしか誘導されない-ゼブラフィッシュ外胚葉に存在する応答能の差-" 細胞工学. 16. 373-38 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ryoske Makita: "Zebrafish wntll : pattern and regulation of the expression by the yolk cell and No tail activity" Mechanisms of Development. 71. 165-176 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sumito Koshida: "Initial anteroposterior pattern of zebrafish central nervous system is determined by differential competence of the epiblast" Development. 125. 1957-1966 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Etsuro Yamaha: "Dorsal specification in the blastoderm at the blastula stage in goldfish, Carassius auratus" Development, Growth and Differentiation. 40. 267-275 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 武田洋幸: "実験生物ものがたり: ゼブラフィッシュ" 遺伝. 52. 68-71 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 武田洋幸: "ゼブラフィッシュの現状: 突然変異体から遺伝子へ" 医学のあゆみ. 185. 395-398 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makoto Kobayashi: "Overexpression of the forebrain-specific homeobox gene six3 induces rostral forebrain enlargement in zebrafish" Development. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yojiro Yamanaka: "A novel homeobox gene, dharma, can induce the organizer in a non-cell autonomous manner" Genes & Development. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toshiro Mizuno: "Cell and tissue transplantation in zebrafish embryos" Molecular Methods in Developmental Biology-Xenopus and Zebrafish. ed. M.Guille. Humana Press Inc.(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyagawa, T.Amanuma, H., Kuroiwa, A.& Takeda, H.: "Specification of posterior midbrain region in zebrafish neuroepithelium" GENES TO CELLS. 1. 369-377 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuno, T., Yamaha, E., Wakahara, M., Kuroiwa, A., & Takeda, H.: "Mesoderm induction in zebrafish" Nature. 383. 131-132 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makita, R., Mizuno, T., Kuroiwa, A.& Takeda H.: "Zebrafish wntll : pattern and regulation of the expression by the yolk cell and No tail activity" Mech.Dev. 71. 165-176 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamaha, E., Mizuno, T., Hasebe, Y., Takeda, H.& Yamazaki, F.: "Dorsal specification in the blastoderm at the blastula stage in goldfish, Carassius auratus" Develop.Growth Differ. 40. 267-275 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Koshida, S., Shinya, M., Mizuno, T., Kuroiwa, A.& Takeda, H.: "Initial anteroposterior pattern of zebrafish central nervous system is determined by differential competence of the epiblast" Development. 125. 1957-1966 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuno, T., Shinya, M.& Takeda, T.: "Cell and tissue transplantation in zebrafish embryos. In Molecular Methods in Developmental Biology-Xenopus and Zebrafish" Humana Press Inc. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi, M., Toyama, R., Takeda, H., Dawid, I.B.& Kawakami, K.: "Overexpression of the forebrain-specific homeobox gene six3 induces rostral forebrain enlargement in zebrafish." Development. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamanaka, T., Mizuno, T., Sasai, Y., Kishi, M., Takeda, H., Kim, C., Hibi, M., & Hirano, T.: "A novel homeobox gene, dharma, can induce the organizer in a non-cell autonomous manner." Genes & Development. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 武田 洋幸: "頭部神経は外胚葉の前方でしか誘導されない-ゼブラフィッシュ外胚葉に存在する応答能の差-" 細胞工学. 16. 373-381 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toshiro Mizuno: "Cell and tissue transplantation in zebrafish embryos" Molecular Methods in Developmental Biology-Xenopus and Zebrafish.ed.M.Guille.Humana Press Inc.(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Etsuro Yamaha: "Dorsal specification in the blast oderm at the blastula stage in goldfish,Carassius auratus" Development, Growth and Differentiation. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ryoske Makita: "Zebrafish wnt11:pattern and regulation of the expression by the yolk cell and No tail activity" Mechanisms of Development. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sumito Koshida: "Initial anteroposterior pattern of zebrafish central nervous system is determined by differential competence of the epiblast" Development. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Terumi Miyagawa: "Specification of posterior midbrain region in zebrafish neuroepithelium" GENES TO CELLS. 1. 369-377 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshiro Mizuno: "Mesoderm induction in zebrafish" Nature. 383. 131-132 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 武田洋幸: "頭部神経は外胚葉の前方でしか誘導されない-ゼブラフィッシュ外胚葉に存在する応答能の差-" 細胞工学. 16(in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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