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Three-dimensional image analysis of migration patterns of mouse neural crest cells

Research Project

Project/Area Number 08680791
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionOsaka University

Principal Investigator

ITO Kazuo  Osaka Univ.Graduate School of Science, Research Associate, 大学院・理学研究科, 助手 (00193475)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsmouse neural crest / migration pattern / three-dimensional image analysis / extracelluar matrix
Research Abstract

We have examined migration patterns of mouse neural crest cells by three-dimensional image analysis using the monoclonal antibody 4E9R to identify neural crest cells. In the trunk, three sclerotomal regions having different migratory pathways of neural crest cells were identified along the anteroposterior axis and the size of these regions temporally changed with a systematic mode. The data suggest that the regional differences of neural crest cell migration within each sclerotome and their temporal changes generate migration patterns of trunk neural crest cells in the mouse. The distribution of chondroitin sulfate proteoglycans in the scletotome was correlated with migration patterns of neural crest cells. Furthermore, their migration patterns were altered in embryos treated with the inhibitors of sulfated proteoglycan biosynthesis, sodium chlorate and beta-D-xyloside. We conclude that systematic spatiotemporally changes of the distribution of chondroitin sulfate proteoglycans in the sclerotome are a key requisite for the formation of migration patterns of mouse trunk neural crest cells. The role of tenascin in neural crest cell migration has been controversial. Migration patterns of these cells in tenascin-knockout mouse embryos were comparable with those of wild-type crest cells. This support the notion that tenascin is not directly responsible for the segmented outgrowth of trunk neural crest cells. In the cephalic region, mesencephalic neural crest cells migrated in the specific portion, that is in the mesenchyme proximal to the boundary between the midbrain and the hindbrain.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 久保田 幸彦: "New monoclonal antibody(4E9R)identifies mouse neural crest cells" Developmental Dynamics. 206. 368-378 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yukihiko Kubota, Toshiteru Morita and Kazuo Ito: "New monoclonal antibody (4E9R) identifies mouse neural crest cells" Developmental Dynamics. vol.206. 368-378 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 久保田幸彦: "New monoclonal antibody (4E9R) identifies mouse neural crest cells" Developmental Dynamics. 206. 368-378 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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