Analysis of mutants concerning sexual morphogenesis of the basidiomycete Corpinus cinereus.
| Project/Area Number |
08680794
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Okayama University |
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Principal Investigator |
KAMADA Takashi Okayama University, Faculty of Science, Professor, 理学部, 教授 (40033360)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | basidiomycetes / Coprinus cinereus / clamp connection / dikaryon / HMG / mating-type gene / transcription factor |
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| Research Abstract |
1. The basidiomycete Corpinus cinereus is a tetrapolar species in which mating type is determined by genes at two unlinked loci known as A and B.A recessive mutation in a gene designated pcc1 (pseudoclamp connection formation ) of C.cinereus, which leads to pseudoclamp conneciton formation, A-regulated sexual morphogenesis, whthout mating was analyzed. Cloning and sequencing of the pcc1 gene and cDNA identified an ORF of 1683 bp interrupted by one intron. Database serches revealed that pcc1 encodes an SRY-type HMG protein. Northern analysis revealed that the level of pcc1 expression is higher in the kikaryon, an A-on B-off homokaryon and an A-off B-on homokaryon than in A-off B-off homokaryon. Sequencing of the pcc1-1 mutant allele revealedt that the mutant carries a nonsense mutation at serine 211, a residue located between the HMG box and the C-terminus.
2. Two kinds of mutants concerning clamp connection formation in C.cinereus were newly isolated from an AmutBmut strain, which forms clamp connections without mating, by REMI (restriction enzyme mediated integration) : one fails to form clamp connections ; the other forms abnormally shaped clamp connections.
3. We constructed a common-AB diploid strain of Coprinus cinereus and mated this to a compatible hoplid strain to construct a diploid-haploid dikaryon. We examined the positions of the diploid and haploid nuclei in the apical and subapical cells of the dikaryon by flourescence microscopy and microfluorometry. The results obtained revealed that the two nuclei alternate in taking the leading and second positions in the apical cell at almost every conjugate division in the dikaryon.
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Report
(3 results)
Research Products
(6 results)
