| Project/Area Number |
08680830
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Tokyo Institute of Psychiatry |
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Principal Investigator |
IKEDA Kenji Tokyo Institute of Psychiatry, Research Scientist, 神経病理部門, 参事研究員 (90232181)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUTAKE Satoshi Tokyo Institute of Psychiatry, Research Scientist, 分子生物部門, 主任研究員 (50090402)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | glial tangle / oligodendroglia / astrocytes / argyrophilic threads / neurofibrillary tangles / glial fibrillary tangles / neurofibrillary tangles / タウタンパク / 異常リン酸化 / タウ附属タンパク / 培養細胞 |
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| Research Abstract |
Tau-positive inclusions that occur in glial cells are called glial fibrillary tangles or, more simply, glial tangles. These include tuft-shaped astrocytes, thorn-shaped astrocytes, coiled bodies and argyrophilic threads. The latter two sltructures occur in oligodendroglia. The tau protein in glial tangles is hyperphosphorylated and has similar immunohistochemical profiles to that in neurofibrillary tangles (NFTs) except that there are no epitopes derived from alternatively spliced exon 2 and 3. In contrast to NFTs, glial tangles rarely show solid filaments. Such NFTs-associated molecules as ubiquitin, apolipoprotein E,alpha1-antichymotrypsin and heparan sulfate are all absent from glial tangles. These characteristics suggest that glial tangles resemble the pretangles which occur in neurons adn are thought to represent an early stage of NFTs. Tau pathology in neurodegenerative diseases takes heterogenous forms.
Manifestation of partially phosphorylated tau in rat primary culture astrocytes was shown immunohistochemically by phosphorylated as well as non-phosphorylated tau antibodies and by immunoblots. Immunogenicity for tau gradually diminished during further culture、however, continuous and recurrent exposure to calcium ionophore maintained immunoreactivity for tau in culture astrocytes and moreover occurred phosphorylation at Ser 396. Electron microscopy showed tau distributed diffusely in the experimental astrocytes except for in the nucleus and mitochondria but no filamentous assembly was observed.
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