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STRUCTURE,DISTRIBUTION AND PHARMACOLOGICAL PROPERTIES OF GABA RECEPTO rho SUBUNITS.

Research Project

Project/Area Number 08680831
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionIWATE UNIVERSITY

Principal Investigator

OGURUSU Tato  FACULTY OF ENGINEERING,DEPARTMENT OF COMPUTER AND INFORMATION SCIENCE,ASSOCIATE PROFESSOR, 工学部, 助教授 (70177202)

Co-Investigator(Kenkyū-buntansha) SAKATA Kazumi  FACULTY OF ENGINEERING,DEPARTMENT OF COMPUTER AND INFORMATION SCIENCE,ASSISTANT, 工学部, 助手 (80261163)
SHINGAI Ryuzo  FACULTY OF ENGINEERING,DEPARTMENT OF COMPUTER AND INFORMATION SCIENCE,PROFESSOR, 工学部, 教授 (00089088)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsGABA receptor rho subunit / rho-containing receptor / rat brain / picrotoxinin / Xenopus oocytes / in situ hybridization / RT-PCR / 塩素イオンチャンネル / GABA受容体ρサブユニット / r-aminobutyric acid / GABAc Receptor / ρ subunit / bicucu IIine / picrotoxin / in situ hybridization / Xenopus oocyte
Research Abstract

(1) We used digoxigenin-labelled single strand DNA probes to examine the expression of the mRNA encoding GABA receptor p 3 subunit in sections of the adult rat retina. Transcript for the p 3 subunit was found in cell somata of a portion of cells lying in the ganglion cell layer.
(2) Homooligomeric rat GABA p 3 receptors were expressed in Xenopus oocytes, and their pharmacological profile was investigated electrophysiologically. GABA activated the rho 3 receptors with EC5O value of 7.5 mu M and a Hill coeffcient of 1.6. The GABA-induced current was not antagonized by bicuculline, but was blocked by picrotoxin. The current was almost insensitive to pentobarbitral, diazepam and a neurosteroid, 3 a-OH-DHP.When the heterooligomeric p 2 p 3 receptors were expressed, picrotoxinin sensitivity was significantly reduced.
(3) RT-PCR analysis revealed that the expression of rho 2 mRNA in adult rat brain was 7.7 times higher than mRNA in the rat brain at embryonic day 16, while that of rho 3 in the embryonic brain was 5.9 times higher than in the adult brain. In the adult rat brain the rho 3 mRNA was present in the mesencephalon, hippocampus, cerebelllum, thalamus. and basal ganglia. In situ hybridization has been used to demonstrate the presence of rho 2 mRNA in the hippocampal CAl region of the 8-day-old rat, and in the CAl region of the hippocampus, lateral geniculate nucleus, superficial gray layer of the superior colliculus and the pars compacta of the substantia nigra of the adult rat.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Shingai R. et al.: "Functional expression of GABA p3 receptors in Xenopus oocytes" Neuroscience Research. 26. 387-390 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogurusu T. et al.: "Localization of GABA receptor P3 subunit in rat retina" Neuro Repcrt. 8. 925-927 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogurusa T. et al.: "Localization of GABA receptor P3 subunit in rat retina" J.Neurochem.Supplement. 69. S238A- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shingai R.et al.: "Functional expression of GABA rho 3 receptors in Xenopus oocytes." Neurosciene Research. 26. 387-390 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogurusu T.et al.: "Localization of GABA receptor rho 3 subunit in rat retina." Neuro Report. 8. 925-927 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogurusu T.et al.: "Localization of GABA receptor rho 3 subunit in rat retina." Journal Neurochemistry. Supplement 69. S238A (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogurusu T.et al.: "Localization of GABA receptor ρ3 subunit in rat retina" Neuro Report. 8. 925-927 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ogurusu T.et al.: "Localization of GABA receptor ρ3 subunit in rat retina" J.Neurochem.Supplement. 69. S238A- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ryuso Shingai 他: "Functional expression of GABA ρ3 receptors in Xenopus oocytes" Neuroscience Research. 26. 387-390 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tarou Ogurusu 他: "Localization of GABA receptor ρ3 subunit in rat retina" Neuro Report. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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