| Project/Area Number |
08680902
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tohoku University |
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Principal Investigator |
MIYOSHI Ichiro Tohoku University, School of Medicine, Research Associate, 医学部, 助手 (10183972)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Noriyuki Tohoku University, School of Medicine, Professor, 医学部, 教授 (60001947)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Model Mouse / Transgenic Mouse / Chromosome Deletion / Triplet (Trinucleotide) Repeat |
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| Research Abstract |
To gain insight into the association of a chromosome deletion with a fragile site localized to the p (CCG) repeat which expand with transmission and to develop an animal model of this disorder, we have generated transgenic mice carrying p (CCG) repeats in the transgene. Uninterrupted 30-, 60-, and 100-repeats p (CCG) DNA fragment were amplified by PCR using CCG and CGG trinucleotide repeat oligomers (40repeat) as both primers and template. Constructs were prepared by ligation of these synthesized p (CCG) DNA fragments into the 5'-untranslated region of LacZ high expression vector composed of cytomegalovirus enhancer, beta-actin promoter, beta-globin intron, LacZ and polyA signal sequence. Regardless of the number of repeat used, these transgenes containing the CCG repeat in transgenic mice showed on change in repeat length with transmission. Neither the chromosome deletion nor phenotypic effects of the transgenes were observed in transgenic mice. Therefore, we may need to consider the use of longer CCG repeat in the constructs or factors other than trinucleotide repeat length alone to explain CCG instability and create a model mouse.
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