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The Effect of Oral Live Poliovaccines Using Human Poliovirus Receptor Carrying Transgenic Mice

Research Project

Project/Area Number 08680912
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionKITASATO UNIVERSITY

Principal Investigator

TAMAUCHI Hidekazu (1997-1998)  Kitasato Univ., School of Medicines, Research Associate, 医学部, 助手 (60188414)

寺田 英司 (1996)  北里大学, 理学部, 助教授 (10113440)

Co-Investigator(Kenkyū-buntansha) 玉内 秀一  北里大学, 医学部, 助手 (60188414)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordspoliovirus / oral immunization / transgenicmouse / mucosal immunity / cytokines / peyer's patches / IEL / IgA / Tgマウス / 経口ワクチン
Research Abstract

Oral poliomielitis vaccine is one of the safest and most effective vaccines used in humans, as showen by the successful eradiation of paralytic cases caused by wild virulent viruse in many developed countries, but there is not the other evaluated system than the using monkey model, because of the most poliovirus are species specific. And a monkey model, however, has inherent disadavantages as follows : (1) difficulty in handling, (2) high cost of breeeding, (3) high risk of zoonosis, (4) endangered status of monkeys in natural environments.
We study that the effect of oral live poliovaccine using the transgenic mouse carrying human poliovirus receptor gene (PVR-Tg21). The MN341 derived from virulent Mohoney strain of poliovirus type 1 (MN341) and the attenuated Sabin 1 strain of poliovirus type 1 were used in this study. The PVR-Tg21 mice, orally immunized with the attenuated Sabin 1 strain of poliovirus type 1, were linoculated with MN341. (1) All non-immunized PVR-Tg mice were dead at … More 7dasys after the inoculation of MN341 but all immunized PVR-Tg21 mice were escaped from the death. (2) In the feces and serum of PVR-Tg2l mice with the oral- mmunization of atteneated Sabin I strain, we detected the poliovirus specific IgA and neutralization antibogy. (4) In the same PVR-Tg21 mice, the titer of poliovirus was not recognized in feces and serum. (5) The cell numberof Tlymphocytes in Peyers' pathes and tntraepithelial of small intestin were decreased at the ldays after the inoculation of MN341. But the cell number was recoverd until the 10 days. (6) The considalable CD4 cells in Peyer's patches of MN341 inoculated PVR-Tg2l mice were differanciated the IL-4 producing cells. These results indicated that the symptons of PVR-1g21 mice was similar to thata of poliovirus inoculated primates. ft is become a new animal model for study of poliovirus infection. Thus, the transgenic mice, PVR-Tg2l, may become a new animal mpdel in place of monkeys for safety testing of oral poliovirus vaccine preparations. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Koichi Koyama: "B-cell activation in the mesenteric lymph nodes of resistant BALB/c mice infected with the murin nematode parasite Trichuris muris." Prasitol. Res.85. 194-199 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seiji Ohka: "Retrograde transport of intact Poliovirus through the axon via the fast transport system." Virology.250. 67-75 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hideaki Ishii: "In the absence of Epstein-Barr virus infection, Phorbol seter modulaters apoptosis in cycloheximide-treated Burkitt' lymphoma (BJA-B) cells." Int. J. Exp. Pathol.78 (3). 123-131 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoh Dobashi :"Cell cycle control with minimal participation of Cdk2 in murine fibrosarcoma clone cultured in protein-freemedium." Biochemical and biophysical reserch communications. 232. 622-625 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koichi Kubota: "Generation of novel killer hybridomas derived from proliferation-suppressed somatic cell hybrids between YACUTT cell hymphoma and normal lymphocytes actiavted in secondary mixed lymphocytes cultures." J. Immunol. Methods.192. 137-147 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koichi Kubota: "Deregulated c-myc expression in sufficient for emeraence of the tumorigenic phenotype in malignancy-suppressed in tratypic T-cell hybrids : an in vitro for multistep-lymphomagenesis." Cancer Letter.101. 199-203 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koichi Koyama: "B-cell activation in the mesenteric lymph nodes of resistant BALB/c mice infected with the murine nematode parasite Trichuris muris." Prasitol.Res.85. 194-199 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seiji Ohka: "Retrograde transport of intact Poliovirus through the axon via the fast transport system." Virology. 250. 67-75 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hideaki Ishii: "In the absence of Epstein-Barr virus infection, Phorbol seter modulaters apoptosis in cycloheximide-treated Burkitt' lymphoma (BJA-B) cells." Int.J.Exp.Pathol.78 (3). 123-131 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoh Dobashi: "Cell cycle control with minimal participation of Cdk2 in murine fibrosarcoma clone cultured in protein-free medium." Biochemical and biophysical reserch communications. 232. 622-625 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koichi Kubota: "Generation of novel killer hybridomas derived from proliferation-suppressed somatic cell hybrids between YACUT T cell lymphoma and normal lymphocytes actiavted in secondary mixed lymphocytes cultures." J.Immunol.Methods. 192. 137-147 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koichi Kubota: "Deregulated c-myc expression in sufficient for emergence of the tumorigenic phenotype in malignancy-suppressed intra T-cell hybrids : an in vitro for multistep lymphomagenesis." Cancer Letter. 101. 199-203 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary

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Published: 1996-04-01   Modified: 2016-04-21  

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