| Project/Area Number |
08680923
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TAKAKUDA Kazuo Tokyo Medical and Dental University, Inst Med Dent Engng, Associate Professor, 医用器材研究所, 助教授 (70108223)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Hideaki Tokyo Medical and Dental University, Inst Med Dent Engng, Assistant, 医用器材研究所, 助手 (50014163)
NAGAI Masahiro Tokyo Medical and Dental University, Inst Med Dent Engng, Assistant, 医用器材研究所, 助手 (10013971)
MIYAIRI Hiroo Tokyo Medical and Dental University, Inst Med Dent Engng, Professor, 医用器材研究所, 教授 (50013892)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Cell Culture / Biomaterials / Biocompatibility / Biomechanics / Mechanical Testing |
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| Research Abstract |
In this experiment, we developed a culture system to examine the effect of mechanical stimuli on the collagen gel specimen involving fibroblasts. The magnitude of strains were set to 10% in tension and compression, the frequency was 0.5Hz, 24 hours a day and last 1 or 2 weeks. The mechanical properties of elastic moduli and maximum stress of specimens after testing and the control specimens at static condition were measured by micro tensile testing under microscope. It was found that both indicators of mechanical properties increase in accordance with the culture time in experimental and control specimens. It also found that the strengthening of specimens were more significant in experimental group than control group In mechanical testing we further measure the strains distributions of spesimens by the use of the marker particles which locations were measured by image analyzes. The strenghtening was induced mainly on the central parts of the specimens where the tensile stress condition
… More
s were realized, whereas both ends of the specimens where compressive stress were generated were least strengthened. The degree of the effects of mechanical stimuli differ among the type of the cells. In bone marrow stromal cells, the tensile stresses generated by the cells were very strong and even the specimens in static conditions shrank very much and they detached from the stainless steel t wire mesh holding the gel. On the other hand, the tensile stress generated by the L cells were very small and very little effect of mechanical stimuli were observed.
The in vivo experiments, which were the couterparts of the previous vitro experiments were developed. In this rat model in which a femora-patellar tendon-tibia specimen were fixed in a stainless steel holder and transplanted under the back skin, artificial repetitive tensile stress were applied. The maximum strength of the tendon did not decreased if the mechanical stimuli were applied, but the control specimens under static conditions did. Thus in the connective tissue which carried out mechanical functions in living body, the fate of the tissues is governed by the mechanical environment. It also suggests that the mechanical integrity of the artificial materials and the living tissue is based on the strong binding between collagen and biomaterials, and since the cells especially fibroblasts and osteoblasts generate tensile stress which may datatch them at the interface, it may result in the differences in biocompatibilty among the tissues and materials. Less
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