| Project/Area Number |
08680926
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SATO Hiroko Kyoto University, Department of Polymer Chemistry, Instructor, 工学研究科, 助手 (00093245)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Hiroshi Kyoto University, Department of Electronics Science and Engineering Instructor, 工学研究科, 助手 (20127103)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | negative ion implantation (NIABNIS) / silver negative ions / tissue culture polystyrene / material surfaces / vascular endo-thelial cells / neural cells / hydrophilicity / hydrophobicitv / cell adhesion-proteins / 注入エネルギー / 接触角測定 / 材料表面物性 / 親水,疎水性 / ポリスチレン / 負イオン注入法 / 細胞培養用ポリスチレン(TCPS) / 生育細胞密度 |
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| Research Abstract |
The purpose of this research is to study and develop on negative ion-implanted materials which assist and induce to repair and regenerate wounded biological tissues, such as vascular blood vessels and neural systems. Silver negative ions (Ag^-) were chosen first to be implanted to polystyrene (PS) and tissue culture polystyrene (TCPS) by using neutral and ionized alkaline bombardment-type heavy negative ion sources (NIABNIS) in doses of 10^<14> to 10^<16> ions "Jm^<-2> at relatively low ion energies of 5-30 keV.The surface property and biocompatability of Ag^--implanted TCPS and PS were investigated. Surfaces of Ag^--implanted PS were studied by means of secondary ion mass spectrometry (SIMS), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and micro-Raman spectroscopy, and contact angle measurement. As a result of Ag^--implantation, the polystyrene surfaces underwent degradation, thereby becoming more hydrophilic.
The cell density of vascular endothelial cells (HUVEC) was observed to decrease remarkably on Ag^--implanted TCPS, compared with that on TCPS, while the cell density was observed to increase extremely on Ag^--implanted PS than on the untreated PS region. In addition, the cells were observed to be induced on Ag^--implanted PS region from PS region, and to be lined up in parallel on the TCPS region contiguous to the Ag^--implanted TCPS region in the shape of stripe. Murine-derived neural cells were observed to settle on the Ag^--implanted PS region in the manner same as vascular endothelial cells. In addition, the neurite outgrowth was also observed to develop specifically on the Ag^--implanted PS region. There was no significant difference for the culture of neural cells among on C^-, Cu^-, and Ag^--implanted PS and TCPS, although the mechanism for effects on cells is unclear and the further study is necessary.
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