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Study on negative ion-implanted biomaterials to induce vascular endothelial cells and neural cells

Research Project

Project/Area Number 08680926
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SATO Hiroko  Kyoto University, Department of Polymer Chemistry, Instructor, 工学研究科, 助手 (00093245)

Co-Investigator(Kenkyū-buntansha) TSUJI Hiroshi  Kyoto University, Department of Electronics Science and Engineering Instructor, 工学研究科, 助手 (20127103)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsnegative ion implantation (NIABNIS) / silver negative ions / tissue culture polystyrene / material surfaces / vascular endo-thelial cells / neural cells / hydrophilicity / hydrophobicitv / cell adhesion-proteins / 注入エネルギー / 接触角測定 / 材料表面物性 / 親水,疎水性 / ポリスチレン / 負イオン注入法 / 細胞培養用ポリスチレン(TCPS) / 生育細胞密度
Research Abstract

The purpose of this research is to study and develop on negative ion-implanted materials which assist and induce to repair and regenerate wounded biological tissues, such as vascular blood vessels and neural systems. Silver negative ions (Ag^-) were chosen first to be implanted to polystyrene (PS) and tissue culture polystyrene (TCPS) by using neutral and ionized alkaline bombardment-type heavy negative ion sources (NIABNIS) in doses of 10^<14> to 10^<16> ions "Jm^<-2> at relatively low ion energies of 5-30 keV.The surface property and biocompatability of Ag^--implanted TCPS and PS were investigated. Surfaces of Ag^--implanted PS were studied by means of secondary ion mass spectrometry (SIMS), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and micro-Raman spectroscopy, and contact angle measurement. As a result of Ag^--implantation, the polystyrene surfaces underwent degradation, thereby becoming more hydrophilic.
The cell density of vascular endothelial cells (HUVEC) was observed to decrease remarkably on Ag^--implanted TCPS, compared with that on TCPS, while the cell density was observed to increase extremely on Ag^--implanted PS than on the untreated PS region. In addition, the cells were observed to be induced on Ag^--implanted PS region from PS region, and to be lined up in parallel on the TCPS region contiguous to the Ag^--implanted TCPS region in the shape of stripe. Murine-derived neural cells were observed to settle on the Ag^--implanted PS region in the manner same as vascular endothelial cells. In addition, the neurite outgrowth was also observed to develop specifically on the Ag^--implanted PS region. There was no significant difference for the culture of neural cells among on C^-, Cu^-, and Ag^--implanted PS and TCPS, although the mechanism for effects on cells is unclear and the further study is necessary.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] H.Sato: "Surface modification of polystyrene surface by silver-negative ion implantation for improving surface wettability and cell adhesion property." Proc.the 8th Symp.Beam Engin.Advanced Mater.Syntheses Includ.Bio-Med.Mater.& Treatment. BEAMS 1997. 109-112 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Contact angle lowering of polystyrene surface by silver-negative-ion implantation for improving biocompatibility and introduced atomic bond evaluation by XPS." Nuclear Instr.Methods Phys.Res.(NIM B). B141. 197-201 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Surface modification by silver-negative-ion implantation for controlling cell-adhesion property of polystyrene." Surface Coating and Technol.103/104. 124-128 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Enhanced growth of human vascular endothelial cells on negative ion(Ag^-)-implanted hydrophobic surfaces." J.Biomed.Mater.Res.44. 22-30 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 佐藤弘子: "負イオン注入によるポリスチレンにおける神経細胞の接着特性の改質と分化誘導" 第9回粒子線の先端的応用技術に関するシンポジウム論文集. BEAMS 1998. 87-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Negative-ion beam surface modification of tissue-culture polstyrene dishes for changing hydrophilic and cell-attachment properties." Nuclear Instr.Methods Phys.Res.(NIMB). B148. 1133-1140 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Surface modification of polystyrene surface by silver-negative ion implantation for improving surface wettability and cell adhesion property." Proc.the 8th Symp Beam Engin. Advanced Mater. Synthes. Includ. Bio-Med. Mater. & Treatment BEAMS1997. 109-112 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Contact angle lowering of polystyrene surface by silver-negative-ion implantation for improving biocompatibility and introduced atomic bond evaluation by XPS." Nuclear Instr.Methods Phys.Res.(NIM B). B141. 197-201 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato and J.Ishikawa: "Surface modification by silver-negative-ion implantation for controlling cell-adhesion property of polystyrene." Surface Coating and Technol.103/104. 124-128 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Improvement of neuron attachment properties of polystyrene by silver-negative implantation and induction of neural protrusion outgrowth." Proc.the 8th Symp Beam Engin. Advanced Mater. Synthes Includ. Bio-Med. Mater. & Treatment BEAMS1998. 87-90 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Enhanced growth of human vascular endothelial cells on negative ion (Ag^-)-implanted hydrophobic surfaces." J.Biomed. Mater. Res.44. 22-30 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato: "Negative-ion beam surface modification of tissue-culture polystyrene dishes for changing hydrophilic and cell-attachment properties." Nuclear Instr.Methods Phys.Res.(NIMB). B148. 1133-1140 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Sato,H.Tsuji,S.Ikeda N.Ikemoto,J.Ishikawa,and S.Nishimoto: "Enhanced growth of human vascular endothelial cells on negative ion(Ag^-)-implanted hydrophobic surfaces" J.Biomed.Mater.Res.44,1. 22-30 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Tsuji,H.Sato,S.Ikeda N.Ikemoto,Y.Gotoh,and J.Ishikawa: "Surface modification by silver-negative-ion implanteation for controlling cell-adhesion property of polystyrene." Surface Coating and Technol.103-104. 124-128 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Tsuji,H.Sato,S.Ikeda Y.Gotoh,and J.Ishikawa: "Contact angle lowering of polystyrene surface by silver-negative-ion implantation for improving biocompatibility and introduced atomic bond evaluation by xps(NIM B)" Nuclear Instr.Methods Phys.Res.B141. 197-201 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Ikemura,H.Tsuji,H.Sato,S.Ikeda,N.Ikemoto,Y.Gotoh and J.Ishikawa: "Surface modofocation of polystyrene surface by silver-negative-ion implantation for improving surface wettability and cell adhesion property." Rroc.the 8th Symp Beam Engineer Advaced Mater Syntheses includ Bio-Ned Mater & Treatment sBEAMS. 8. 109-112 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Tsuji, H.Sato, S.Ikeda, N.Ikemoto, Y.Gotoh, and J.Ishikawa: "Surface Modification by Silver-Negative-Ion Implantation for Controlling Cell-Adhesion Property of Polystyrene." Nuclear Instr.Methods Phys.Res.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Tsuji, H.Sato, S.Ikeda, Y.Gotoh, and J.Ishikawa: "Contact Angle Lowering of Polystyrene Surface by Silver-Negative-Ion Implantation for Improving Biocompatibility and Introduced Atomic Bond Evaluation by XPS." Surface Coating and Technol. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Ikemura, H.Tsuji, H.Sato, S.Ikeda, N.Ikemoto, Y.Gotoh, and J.Ishikawa: "Surface modification of Polystyrene Surface by Silver-Negative-Ion Implantation for Improving Surface Wettability and Cell Adhesion Property" Proceeding of the Eighth Symposium on Beam Engineering of Advanced Material Syntheses Including Bio-Medical Materials and Treatments. 8. 109-112 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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