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in vivo Gene Delivery System using Water-soluble Carrier.

Research Project

Project/Area Number 08680928
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKyoto Institute of Technology

Principal Investigator

MURAKAMI Akira  Kyoto Institute of Technology, Department of Polymer Science and Engineering, Professor, 繊維学部, 教授 (60210001)

Co-Investigator(Kenkyū-buntansha) YAMAOKA Tetsuji  Kyoto Institute of Technology, Department of Polymer Science and Engineering, Le, 繊維学部, 講師 (50243126)
MIYAMOTO Masatoshi  Kyoto Institute of Technology, Department of Polymer Science and Engineering, As, 繊維学部, 助教授 (70149524)
KIMURA Yoshihatu  Kyoto Institute of Technology, Department of Polymer Science and Engineering, Pr, 繊維学部, 教授 (10132276)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsplasmid / gene transfection / gene carrier / cationic polymer / DDS / DEAE / 蛍光異方性
Research Abstract

In order to examine the expression of genes after transfection of genes to a body, it is quite important to construct plasmids which can transfect genes. For this purpose, instead of SV 40 Early-Promoter, which has been used by now, we constructed a novel plasmid having lac-z gene in the down stream region ofbeta-actin Promoter or CMV Promoter. As gene-carriers, we developed two types of water soluble polymers ; (1) Poly (vinyl alcohol) with tertiary aminoacetal moieties, (2) Copoly (dimethylaminopropylacrylamide-acrylamide). Using these gene carriers, the newly constructed plasmid was transfected to NIH3T3 cells, and the transfected genes expressed in high levels. These results suggest that the newly constructed plasmid is applicable to in vivo gene transfection and that the newly developed water-soluble cationic polymers is also potent as gene carriers.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Y.Yamana: "An oligonucleotide derivative possessing an intercalating dye at sugar residue as an RNA specific fluorescent probe" Nucleic Acids Symposium Series. 35巻. 107-108 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Iwase: "Gene Regulation by Decoy Approach(I)Synthesis and Properties of Photo-crosslinked Oligonucleotides." Nucleic Acids Symposium Series. 35巻. 203-204 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Iwase: "Study of RNA structure by pyrene-labeled oligonucleotides" Nucleic Acids Symposium Series. 35巻. 205-206 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Nakaoka: "Prolongation of the serum half-life period of superoxide dismutase by poly(ethylene glycol)modification" J.Controlled Release. 46巻. 253-261 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Tamura: "Preparation of Stereoregulated Antisense Oligodeoxyribonucleoside Phosphorothioate and Interaction with Its complementary DNA and RNA" Nucleosides and Nucleotides. 16巻(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 村上 章: "遺伝子工学技術の発展に伴う核酸ドラッグの現況と課題" 日本臨床. 56巻(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 村上 章: "分子生物学実験講座(共著)" メディカルレビュー社, 146 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yamana: "An oligonucleotide derivative possessing an intercalating dye at sugar residue as an RNA specific fluorescent probe" Nucleic Acids Symposium Series. 35. 107-108 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R,Iwase: "Gene regulation by decoy approach (I) Synthesis and properties of photo-crosslinked oligonucleotides." Nucleic Acids Symposium Series. 35. 203-204 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Iwase: "Study of RNA structure by pyrene-labeled oligonucleotides" Nucleic Acids Symposium Series. 35. 205-206 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Nakaoka: "Prolongation of the serum half-life period of superoxide dismutase by poly (ethylene glycol) modification." J.Controlled Release. 46. 253-261 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Tamura: "Preparation of stereoregulated antisense oligoribonucleoside phosphorothioate and interaction with its complementary DNA and RNA" Nucleosides and Nucleotides. 16, in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Murakami: "Recent development of nucleic acid drug." Japanese Journal of Clinical Medicine. 56. 563-572 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yamana: "An oligonucleotide derivative possessing an intercalating dye at sugar residue as an RNA specific fluorescent probe" Nucleic Acids Symposium Series. 35巻. 107-108 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] R.Iwase: "Gene Regulation by Decoy Approach(I) Synthesis and Properties of Photo-crosslinked Oligonucleotides." Nucleic Acids Symposium Series. 35巻. 203-204 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] R.Iwase: "Study of RNA structure by pyrene-labeled oligonucleotides" Nucleic Acids Symposium Series. 35巻. 205-206 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] R.Nakaoka: "Prolongation of the serum half-life period of superoxide dismutase by poly (ethylene glycol) modification" J.Controlled Release. 46巻. 253-261 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Tamura: "Preparation of Stereoregulated Antisense Oligodeoxyribonucleoside Phosphorothioate and Interaction with Its complementary DNA and RNA" Nucleosides and Nucleotides. 16巻(印刷中). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 村上 章: "遺伝子工学技術の発展に伴う核酸ドラッグの現況と課題" 日本臨床. 56巻(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Yamagami: "Growth Inhibition of Human Leukemic Cells by WT-1(Wilms Tumore Gene)Antisense Oligonucleotides:Implications for the Involvement of WT-1 in Leukemogenesis" Blood. 87. 2878-2884 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 村上 章: "アンチセンス法を用いて遺伝子の機能を制御しよう" 化学. 51. 116-119 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 村上 章: "アンチセンス核酸による治療の基礎" 呼吸. 15. 186-192 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 村上 章: "アンチセンス研究はどこまで進展したか" 化学と生物. 34. 454-460 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Yamana: "An oligonucleotide derivative possessing an intercalating dye at sugar residue as an RNA specific fluorescent probe." Nucleic Acids Sympposium Series. 35. 107-108 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] R.Iwase: "Fluorescent-labeled oligonucleotides for studies on gene structure" Nucleic Acids Symposium Series. 35. 117-118 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Murakami: "Interaction of Antisense Oligonucleotides with DNA and Proteins Studied by Fluorescence Polarization Spectroscopy" Biotechnologia. 4. 153-166 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 村上 章: "アンチセンス法-遺伝子に働く医薬品開発の可能性-" 日薬理誌(Folia Pharmacol.Jpn.). 108-1. 18p-23p (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 村上 章: "学会出版センター" バイオ・高分子研究法6 高分子化学と核酸の機能デザイン2.高機能修飾核酸の分子設計, 200 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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