| Project/Area Number |
08680931
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | FIRST DEPT.OF PATHOLOGY NARA MEDICAL UNIVERSITY |
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Principal Investigator |
YOSHIKAWA Takafumi First dept.of Pathology Nara Medical University, 医学部・第一病理, 講師 (90275347)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Eiji First dept.of Pathology Nara Medical University, 医学部・第一病理, 助教授 (00111899)
OHGISHI Hajime Dept.of Orthopedics Nara Medical University, 医学部・整形外科, 講師 (80213669)
美島 健二 奈良県立医科大学, 医学部・第一病理, 助手 (50275343)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | c-SRC / alkaline phosphatase / osteocalcin / osteogenesis / biomaterial / tissue engineering / SRC gene / Osteogenesis |
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| Research Abstract |
We investigated in vitro and in vivo osteogenic ability of cultured marrow cells by gene expression analysis of alkaline phosphatase (ALP), osteocalcin (OC) and c-SRC.In the initial stage of bone formation by cultured marrow cells, ALP, OC and c-SRC mRNA could be detected in vivo and in vitro. These are useful as a marker of bone formation to obtain valuable information about osteogenic response of cells on biomaterials. We succeeded in production of cultured bone/ceramic constructs which has high osteogenic potential in vivo. A significant benefit of this method is that the construct can be made with only a small amount of aspirated marrow cells from aged patients with little host morbidity. The analyses of these genes showed that we can assemble an in vitro bone/ceramic construct, and due to the porous framework of the ceramic, the construct has osteogenic potential similar to that of autologous cancellous bone.
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