| Project/Area Number |
08680947
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
OGASAWARA Yasuo Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (10152365)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Masami Kawasaki College of Allied Health Professions, Medical Electronics, Associate Pr, 医用電子技術科, 助教授 (50148699)
YADA Toyotaka Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (00210279)
TSUJIOKA Katsuhiko Kawasaki Medical School, Physiolgy, Professor, 医学部, 教授 (30163801)
KAJIYA Fumihiko Kawasaki Medical School, Medical Engineering, Professor, 医学部, 教授 (70029114)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | alpha-receptor sympathetic nerve stimulation / subendocardial artery / pacing overload / slosh phenomenon / vascular pulsatility / slosh現象 / ニードルプローブ型CCD生体顕微鏡 / 血流マ-カ / 血管形状動態 / 冠微小循環血流 / α交感神経活動 / 冠血管トーヌス / 冠動脈slosh現象 / 心筋虚血 |
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| Research Abstract |
This study is to test our hypothesis that enhancement of alpha-receptor sympathetic nerve stimulation improves subendocardial ischaemia by its inhibitory effect on coronary slosh phenomenon adjusting coronary tonus. We, therefore, directly observed pulsatile diameter changes and microcirculation of subendocardial arterioles with our needle type CCD intravital microscope.
Open chest mongrel dogs under anaesthesia were used in the experiment. We measured intramural blood flow with a 20 Mhz 80-channeled ultrasound Doppler flowmeter after exposing septal branches of the left coronary artery. The needle type CCD intravital microscope was inserted from the left appendage into the left ventricle via mitral valve and the subendocardial arterioles were observed. To make comparison, epicardial arterioles were observed too. Heart rate was controlled by right ventricular pacing. Coronary perfusion pressure was controlled aortic banding. Under various pacing rates (140,200,250 bpm), observation of e
… More
ndocardial arterioles were made while measuring intramural blood flow under the following conditions ; 1) basal condition, 2) continuous intravenous injection of norepinephrine (NE,0.25 mug/kg/min), 3) NE plus alpha-receptor blockade (phenoxybenzamine, 0.5 mg/kg).
At the rate of 140 bpm, alpha-receptor blockade caused remarkable increase of retrograde flow during continuous administration of NE.Diastolic forward flow did not show any significant change. As for the subendocardial flow volume, it increased at the rate of 140 bpm, did not show any change at the rate of 200 bpm and decreased at the rate of 250 bpm. At any heart rate, there was an increase of significan retrograde flow and slosh ratio by alpha-receptor blockade. Pulsatililty of both subendocardium and subepicardium showed significant charges after alpha-receptor blockade.
The needle type CCD intravital microscopy enabled to observe pulsatile diameter changes of subendocardial arterioles of a beating heart under alpha-receptor sympathetic nerve stimulation. It was found that alpha-receptor stimulation inhibited slosh phenomenon and maintained the subendocardial blood flow during tachycardia. Less
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