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Hatching of an estuarine crab : characterization, activity, and putative role of a caseinolytic protease

Research Project

Project/Area Number 08833009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 海洋生物学
Research InstitutionOkayama University

Principal Investigator

SAIGUSA Masayuki  Okayama University, 理学部, 助教授 (80135962)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsMarine crustanceans / Hatching / Caseinolytic protease / Activity and characterization / Ultrastructure / Embryonic envelope / 吸水 / 活性 / 生化学的性質 / 卵浮化 / 海産カニ類 / 活性物質の精製 / OHSS(胚脱落誘発物質) / カゼインを分解するプロラアーゼ / 酵素
Research Abstract

The zoeas of an estuarine crab Sesarma haematocheir (akate-gani) hatch synchronously within 0.5 - 1 h from a female. Three aspects of hatching were studied : caseinolytic activity, morphological changes of the embryonic envelope structure, and environmental factors. Hatch water (i.e., the filtered medium in whcih zoeas have hatched) contains a caseinolytic protease, and the molecular weight of this protease was estimated on gel filtration. Transmission electron microscopy shows that the envelope (1.8 mum thick) is composed of three distinct layrs : an outer (E1), thick middle (E2), and thin inner (E3) layr. The outer two layrs, that are structurally the main components of the envelope, were not morphologically altered during hatching, but the inner layr (E3) was clearly altered between 11 h and 3 h before hatching, suggesting its digestion. The assay with isolates of the embryonic envelopes and morphological observations also suggested that the envelope is digested by the hatch water. The time of hatching was strongly affected by low water temperatures, and their effects were much stronger to the embryos detached from the female than female-attached embryos. Salinity was ineffective. Mechanisms of hatching based on the digestion of this (E3) layr has been discussed.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] M.Saigusa: "Two kinds of active factor in crab hatch water." Biological Bulletin. 191. 234-240 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Saigusa: "The circatidal clock in an estuarine crab." Benthos Research. 52. 21-33 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Saigusa: "Hatching and its control by the circatidal clock" Proceedings of Arthropodan Emb.Soc.32. 1-23 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Saigusa, O.Kawagoye: "Circatidal rhythm of an intertidal crab" Marine Biology. 129. 87-96 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Oishi, M.Saigusa: "Nighttime emergence patterns in benthic and・・・" J.Oceanography. 53. 611-621 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Saigusa et al.: "Hatching of an estuarine crab" Biological Bulletin. 193 (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sagusa: "Two Kinds of active Factor in crab hatch water." Biological Bulletin. 191. 234-240 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sagusa: "The circatidal clock in an estuarine crab." Benthos research. 52. 21-33 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sagusa: "Hatching and its control by the circatidal clock." Proceedings of Arthropodan Emb.Soc.32. 1-23 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sagusa, O.kawagoya: "Circatidal rhythm of an intertidalcrab." Marine Biology. 129. 87-96 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Oishi, M.Sagusa: "Nighttime emergence patterns in benthic and #." J.Oceanography. 53. 611-621 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sagusa et al: "Hatching of an estuarine crab." Biological Bulletin. 193 (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Saigusa: "Two kinds of active factor in crab hatch water." Biological Bulletin. 191. 234-240 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Saigusa: "The circatidal clock in an estuarine crab." Benthos Research. 52. 21-33 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Saigusa: "Hatching and its control by the circatidal clock" Proceedings of Atrhropodan Emb.Soc.32. 1-23 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Saigusa, O.Kawagoye: "Circatidal rhythm of an intertidal crab" Marine Biology. 129. 87-96 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Oishi, M.Saigusa: "Nighttime emergence patterns in benthic and …" J.Oceanography. 53. 611-621 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Saigusa et al.: "Hatching of an estuarine crab" Biological Bulletin. 193(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Saigusa,M.: "Two kinds of active factor in crab hatch woter" Biological Bulletin. 191. 234-240 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saigusa,M: "The circatidal clock in an estuarine・・・・・・" Benthos Research. 52(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saigusa,M.,Kawagoye,O.: "Circatidal clock of an intertidal・・・・" Marine Biology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saigusa,M.et al.: "Hatching of an estuarine teerestrial・・・・・" Biological Bulletin. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saigusa.M.: "Hatching and its contool of・・・・" Proceedings of Arthropodan Embryol.Soc.Japan. 32(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三枝誠行: "動物胚の卵乳化機構(I)と(II)" 海洋と生物. 18. 120-125,△298-304 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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