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Degration and removal of the proteins in photosystem II during photoinhibition

Research Project

Project/Area Number 08836007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 光生物学
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

YAMAMOTO Yasusi  OKAYAMA UNIVERSITY,Faculty of Science, Professor, 理学部, 教授 (40091251)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsphotosynthesis / photosystem II / photoinhibition / DI protein / turn-over / plant physiology / active oxygen / rodical
Research Abstract

When plants are illuminated with strong light, photoinhibition of photosynthesis takes place. The target of photoinhibition is photosystem II.In the present study, damage, degradation and recovery processes of the reaction-center binding D1 protein in photosystem II during light stress were studied with spinach PS II membranes, PS II core samples and Chlamydomonas cells. The results obtained are as follows ;
(1) An extrinsic 33 kDa protein of photosystem II regulates the damage-degradation step of the D1 protein. The 33-kDa protein decreases the aggregation of D1 and at the same time enhances the degradation of D1.
(2) Active oxygen molecules are involved in the degradation of D1 not only in the reducing-side photoinhibition, but also in the donor-side photoinhibition.
(3) A large conformational change cn be detected by FTIR under the illumination of the PS II samples with strong light, but no siggnificant structural changes in PS II was found in hydrogen peroxide treatment of the PS II samples.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hashimoto, A.: "Unassembled subunits of the photosynthetic oxygen-evolving complex present in the thylakoind lumen are long-lived and assembly-competent." FEBS Letters. 391. 29-34 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hashimoto, A.: "In organello assembly of newly imported oxygen.evolving complex subunits in isolated chloroplasts." The Plant Cell. 9. 441-452 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang, H.: "Fourier-transform infrared spectroscopic study of the effects of hydrogen peroxide and high light on protein conformation of photosystem II" Photosynthesis Research. 52. 215-223 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamamoto, Y.: "The role of an extrinsic 33 kDa protein of photosystem II in the turnover of the yeastion-center binding protein D1 during photoinhibition" Biochemistry. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hashimoto, A.: "Unassembled subunits of the photosynthetic oxygen-evolving complex present in the thylakoid lumen are long-lived and assembly-competent." FEBS Letters. 391. 29-34 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hashimoto, A.: "In organello assembly of newly imported oxygen-evolving complex subunits in isolated chloroplsts." The Plant Cell. 9. 441-452 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang, H.: "Fourier-transform infrared spectroscopic study of the effects of hydrogen peroxide and high light on protein conformation of photosystem II." Photosynthesis Research. 52. 215-223 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamamoto, Y.: "The role of an extrinsic 33kDa protein of photosystem II in the turn-over of the reaction-center binding protein D1 during photoinhibition." Biochemistry. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hashimoto,A.: "Unassembled subunits of the photosynthetic oxygen-evolving complex present in the thylakoid lumeu and long-lived and assembly-competent." FEBS Letters. 391. 29-34 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hashimoto,A.: "In organello assembly of newly imported oxygen-evolving complex subunits in isolated chloroplasts." The Plant Cell. 9. 441-452 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Zhang,H.: "Fourier-transform infrared spectroscopic study of the effects of hydrogen peroxide and high light on protein conformation of photosystem II" Photosynthesis Research. 52. 215-223 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto,Y.: "The role of an extriusic 33 KDa protein of photosystem II in the turn-over of the reaction-center binding protein D1 during photoinhibition." Biochemistry. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hashimoto,A.: "Unassembled subunits of the photosynthetic oxygen-evdving complex present in the thylakoid lumen are long-lived and assembly-competent." FEBS Letters. 391. 29-34 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hashimoto,A.: "In organello assembly of newly imported oxygen-evolving complex subunits in isolated chloroplasts." The Plant Cell. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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