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Molecular mechanism of daikadaptation regulated by rhodopsin phosphorylation

Research Project

Project/Area Number 08836009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 光生物学
Research InstitutionSapporo Medical University

Principal Investigator

OHGURO Hiroshi  Sapporo Medical University, Department of Ophthalmology, assistant professor, 医学部, 講師 (30203748)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Takashi  Sapporo Medical University, Department of Ophthalmology, professor, 医学部, 教授 (60045367)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsrhodopsin / G-protein cerydod reaph / rhodopsin kinase / phosphorylation / retiuitis pigucutosa / adopfation / dephosphorylation / GTP結合蛋白質共役受容体 / 燐酸化 / セリンスレオニンキナーゼ / 視細胞 / 視興奮 / GTP結合蛋白質
Research Abstract

To understnad the relationship between rhodopsin phosphorylation and dark adaptation, kinetics of rhodopsin phosphorylation was investigated under several conditions and obtained following results.
1)In vivo, rhodopsin phosphorylation was occurred at two different sites and the kinetics of phosphorylation and dephosphorylation was different with each others.
2)Rhodopsin phosphorylation was catalyzed only rhodopsin kinase not protein kinase C.
3)The distribution and kinetics of phosphorylation was modified by the truncation of the C-terminus of rhodopsin.
4)The dephosphorylation of the phospho Ser located C-terminus was faster than those located near membrane.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] H.Ohguro et al.: "Structured and eugymatic aspects of rhodopsin phosphorylation" Journal Biological Chemistry. 271. 5215-5224 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Ohguro: "High Levels of rhodopsin phosphorylation in missarse mutations of C-terminal region of rhodopsin" FEBS Letter. 413. 433-435 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大黒 浩: "ロドプシンの燐酸化脱燐酸化の生理的意義と疾病との関係" 日本眼科学会雑誌. 100. 575-581 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大黒 浩 他: "悪性腫瘍随伴網膜症" 日本眼科学会雑誌. 101. 283-287 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大黒 浩: "ロドプシンのリン酸化と脱リン酸化の分子メカニズム" 生物物理. 212. 155-160 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大黒 浩: "視細胞における情報伝達機構" 札幌医学雑誌. 65. 65-72 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Ohguro et al.: "Structual and euzynagic aspects of rhodopsin phosphorylation" Jornal of Biochemial Chemistry. 271. 5215-5224 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Ohguro: "High levels of rhodopsin phosphorylation in missase mutations of C-fermiuel region of rhodopsin" FEB5 Lett. 413. 433-435 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Ohguro et al: "Structural and Enzymatic aspects of rhodopsin phosphorylation" Journal Biological Chemistry. 271. 5215-5224 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Ohguro: "High levels of rhodopsin phosphorylation in misseuse mutations of C-terminal region of rhodopsin" FEBS Letter. 413. 433-435 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大黒 浩: "ロドプシンの燐酸化脱燐酸化の生理的意義と疾病との関係" 日本眼科学会雑誌. 100. 575-581 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大黒 浩 他: "悪性腫瘍随伴網膜症" 日本眼科学会雑誌. 101. 283-287 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大黒 浩: "ロドプシンのリン酸化と脱リン酸化の分子メカニズム" 生物物理. 212. 155-160 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大黒 浩: "視細胞における情報伝達機構" 札幌医学雑誌. 65. 65-72 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohguro et al.: "The difference in the expressions of visual pigments and transducin in photoreceptor cell differentiation." Tohoku J Exp Med. 178. 233-240 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohguro et al.: "Structural and enzymatic aspects of rhodopsin phosphorylation" J Biol Chem. 271. 5215-5224 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩: "10年後に第2眼に発症をみた網膜色素変成症の1例。" 臨床眼科. 50. 1383-1385 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩 他: "ステロイドの局注により退宿をみた眼窩頭蓋内血管種の1例。" 臨床眼科. 50. 1015-1017 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩 他: "血清アレスチン抗体とMS関連疾患。" 神経眼科. 12. 16-20 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩 他: "ボクシング選手にみられた一過性白内障。" 眼科. 38. 429-431 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩 他: "血清アレスチン抗体陽性であった視神経炎の1例。" あたらしい眼科. 13. 303-305 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩: "ロドプシンの燐酸化脱燐酸化の生理的意義と疾病との関係。" 日本眼科学会雑誌. 100. 575-581 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩: "視細胞における情報伝達機構。" 札幌医誌. 65. 65-72 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩: "アレスチンの機能と構造。" 札幌医誌. 65. 159-165 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩 他: "超音波生体顕微鏡により発見された外傷性毛様体解離の1例。" 臨床眼科. 50. 1723-1725 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大黒 浩 他: "眼瞼腫脹および動眼神経麻痺で初発したヘルペス性髄膜炎の1症例。" あたらしい眼科. 13. 809-812 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2017-10-11  

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